B2TDV9 (NADE_BURPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Bphyt_4784

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099012
Regions
	Nucleotide binding	51 – 58	8	ATP By similarity
Sites
	Active site	53	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2TDV9 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 7B35179EEF75C01D	FASTA	286	31,638
	10 20 30 40 50 60 MTQHDPVARQ ASISEEMHIS AEFDAEYEIE RRVAFLANYL RSSGLKTYVL GISGGVDSTT 70 80 90 100 110 120 AGRLAQLAVE RLRAEHYDAH FVAIRLPYGE QKDEADAQQA LRFIRADENL TIDIKPAADA 130 140 150 160 170 180 MLAALDQSGL LYKDESQQDF VHGNIKARQR MIAQYAVASA RAGVVIGTDH AAESVMGFFT 190 200 210 220 230 240 KFGDGGADVL PLTGLNKRRV RAVSKALGAP EALAHKVPTA DLEMLRPQRP DEDAYGIPYD 250 260 270 280 AIDDFLERKP VSDAARETIL RFHEVTRHKR ALPYTPFDWP VQTSGD

References

[1]

"Complete sequence of chromosome 2 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17436 / PsJN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001053 Genomic DNA. Translation: ACD19149.1.
RefSeq	YP_001888519.1. NC_010676.1.
3D structure databases
ProteinModelPortal	B2TDV9.
SMR	B2TDV9. Positions 9-278.
ModBase	Search...
Protein-protein interaction databases
STRING	B2TDV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6279788.
GenomeReviews	Gene locus Bphyt_4784 in contig CP001053_GR.
KEGG	bpy:Bphyt_4784.
PATRIC	19204129. VBIBurPhy117947_0877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BURPP

Accession

Primary (citable) accession number: B2TDV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program