B2TBS1 (METE_BURPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Bphyt_5364

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097821
Sites
	Metal binding	641	1	Zinc By similarity
	Metal binding	643	1	Zinc By similarity
	Metal binding	726	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2TBS1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 046279E5437DD89A	FASTA	764	85,321
	10 20 30 40 50 60 MARTHIPGFP RIGAQRELKF AQESFWRGES DEQYLLGVAR ELRARHWQLQ QDAKLDFVTV 70 80 90 100 110 120 GDFAYYDQML NLSALLGALP QRFGFDAKTL SLARYYELAR GNAAQPAMEM TKWFDTNYHY 130 140 150 160 170 180 LVPELGPQTT FDGGVEWLFD EIDEALALNL PVKPVLIGPI TYLWLSKSHV AGFDRLSLLP 190 200 210 220 230 240 KLVIRYSRLL EKLKQRGIEW VQLDEPALCV DLPVEWLDAF SAAYDVLGTS GVKVLLATYF 250 260 270 280 290 300 ESAAEHAPRV AVLPVAGVHL DLVRAPQQLD AWRAALPKHA VLSAGVIDGR NIWRADLGEI 310 320 330 340 350 360 FESLQALHAE FGERLWVSSS CSLLHVPVSL DAEQKLDADL KSWLAFATEK LGEVATLALA 370 380 390 400 410 420 LRDPAAAEAT LAAADRALDA RRHSSTVVNA LVQKRVAAVS SAMADRQSPF AERNRLQREA 430 440 450 460 470 480 LGLPLLPTTT IGSFPQTPAI RQARAAYKRG ELRALDYLQR MRAEIEIAVR KQEELGLDVL 490 500 510 520 530 540 VHGEAERNDM VEYFGEQLWG YAFTENGWVQ SYGSRCVKPP IIYGDVYRPE PMTVETTRYA 550 560 570 580 590 600 QSLTQRLMKG MLTGPVTMLQ WSFVRDDQPR STTALQLALA IRDEVVDLEK AGIRIIQIDE 610 620 630 640 650 660 PAFREGLPLR RGDWAAYLEW ATRVFRISAA GVADQTQIHT HMCYSEFNDI LPSIAAMDAD 670 680 690 700 710 720 VITIETSRSA MELLDGFGAF AYPNEIGPGV YDIHSPRVPD AQAMQRLLER ACEVIPAERL 730 740 750 760 WVNPDCGLKT RGWPETEAAL TNMVRAAKAL RAKLAAKQPD EVTA

References

[1]

"Complete sequence of chromosome 2 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17436 / PsJN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001053 Genomic DNA. Translation: ACD19723.1.
RefSeq	YP_001889093.1. NC_010676.1.
3D structure databases
ProteinModelPortal	B2TBS1.
SMR	B2TBS1. Positions 3-753.
ModBase	Search...
Protein-protein interaction databases
STRING	B2TBS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6280325.
GenomeReviews	Gene locus Bphyt_5364 in contig CP001053_GR.
KEGG	bpy:Bphyt_5364.
PATRIC	19205419. VBIBurPhy117947_1512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	LWLSKSH.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURPP

Accession

Primary (citable) accession number: B2TBS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program