B2T6L8 (B2T6L8_BURPP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444
EC=3.4.21.92 HAMAP MF_00444

Alternative name(s):
Endopeptidase Clp 2 HAMAP MF_00444

Gene names

Name:	clpP2 HAMAP MF_00444
Ordered Locus Names:	Bphyt_3190

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP] EMBL ACD17582.1

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family. HAMAP MF_00444

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00444
Ligand	ATP-binding HAMAP MF_00444 Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease HAMAP MF_00444
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	108	1	By similarity HAMAP MF_00444
	Active site	133	1	By similarity HAMAP MF_00444

Sequences

	Sequence		Length	Mass (Da)
	B2T6L8 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 1CB47923BB7BF53D	FASTA	215	23,705
	10 20 30 40 50 60 MHSNYPNITG LGLVPTVIEQ SGRGERAYDI YSRLLRERIV FLVGPVNEQS ASLIVAQLLF 70 80 90 100 110 120 LESENPDKDI CFYINSPGGS VYDGLAIYDT MQFIKPEVST LCTGFAASMG TFLLTAGQRG 130 140 150 160 170 180 KRYALPNARI MIHQPSGGSQ GTAADVEIQA KEVLYLRERL NAMMAERTGR SIEAIARDTD 190 200 210 RDNFMSAQSA KTYGLVDEVL ETRAALNVAP YHMDK

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001052 Genomic DNA. Translation: ACD17582.1.
RefSeq	YP_001896806.1. NC_010681.1.
3D structure databases
ProteinModelPortal	B2T6L8.
SMR	B2T6L8. Positions 13-203.
ModBase	Search...
Protein-protein interaction databases
STRING	B2T6L8.
Protein family/group databases
MEROPS	S14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6281274.
GenomeReviews	Gene locus Bphyt_3190 in contig CP001052_GR.
KEGG	bpy:Bphyt_3190.
PATRIC	19216876. VBIBurPhy117947_7186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	KPRIATL.
ProtClustDB	CLSK2314407.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. ClpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B2T6L8_BURPP

Accession

Primary (citable) accession number: B2T6L8

Entry history

Integrated into UniProtKB/TrEMBL:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)