Reviewed, UniProtKB/Swiss-Prot B2T612 (DADA_BURPP)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	Bphyt_2982

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000138646
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	B2T612-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: F1A427A232E8BE00	FASTA	428	46,075
	10 20 30 40 50 60 MRVVVLGSGV VGVTSAYYLA RAGHEVTVID REAGPALETS FANAGQISPG YASPWAAPGV 70 80 90 100 110 120 PLKAVKWMFQ KHAPLAIRLD GTQFQLQWMW QMLQNCTSSR YAVNKGRMVR LAEYSRDCLQ 130 140 150 160 170 180 ALRAETGIQY EGRTGGTLQV FRTQQQFDGA AKDIAVLQEA NVPYELLSPA ELARAEPALA 190 200 210 220 230 240 AVSHKLTGGL RLPGDETGDC QMFTTRLAAL AEELGVKFRY NTPIDALAMA GDRIAGVKCG 250 260 270 280 290 300 EELVRADSFV VALGSYSTQF LSGLVKIPVY PLKGYSITAP IVNEASAPVS TVLDETYKIA 310 320 330 340 350 360 ITRFDDRIRV GGMAEIVGFD KSLRQARRET LELCVNDLFP GGGDTSKATF WTGLRPMTPD 370 380 390 400 410 420 GTPIVGRTPV PNLFLNTGHG TLGWTMSCGS GQLLADVMSG KQPAIKADDL SVHRYLGEVG GAHRPAYA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001052 Genomic DNA. Translation: ACD17376.1.
RefSeq	YP_001896600.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6280748.
GenomeReviews	Gene locus Bphyt_2982 in contig CP001052_GR.
KEGG	bpy:Bphyt_2982.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MFQKHAP.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_BURPP

Accession

Primary (citable) accession number: B2T612

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)