Reviewed, UniProtKB/Swiss-Prot B2T2S5 (3HAO_BURPP)

Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Ordered Locus Names:

Bphyt_1471

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.
Catalytic activity	3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde. HAMAP MF_00825
Cofactor	Binds 2 Fe2+ ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the 3-HAO family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: HAMAP ferrous iron binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	3-hydroxyanthranilate 3,4-dioxygenase HAMAP MF_00825		PRO_1000134549
Sites
	Metal binding	51	1	Iron 1; catalytic By similarity
	Metal binding	57	1	Iron 1; catalytic By similarity
	Metal binding	95	1	Iron 1; catalytic By similarity
	Metal binding	125	1	Iron 2 By similarity
	Metal binding	128	1	Iron 2 By similarity
	Metal binding	162	1	Iron 2 By similarity
	Metal binding	165	1	Iron 2 By similarity
	Binding site	47	1	Dioxygen By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2T2S5-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: C49B1352CAEC0AC2	FASTA	174	20,043
	10 20 30 40 50 60 MLTYGKPFNF QRWIDDHAHL LKPPVGNQQV WQDSDFIVTV VGGPNHRTDY HDDPLEEFFY 70 80 90 100 110 120 QLRGNAYLHL WIDGKRERVD LKEGDVFLLP PHVRHSPQRP EAGSVCLVIE RQRPAGVVDG 130 140 150 160 170 FEWYCDACGH LVYRVEVQLK SIVSDLPPLF NAFYASEEKR RCGHCGHVHP GKAV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001052 Genomic DNA. Translation: ACD15886.1.
RefSeq	YP_001895110.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6282502.
GenomeReviews	Gene locus Bphyt_1471 in contig CP001052_GR.
KEGG	bpy:Bphyt_1471.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RHSPQRP.
Family and domain databases
HAMAP	MF_00825. [Tree]
InterPro	IPR010329. 3hydroanth_dOase. [Graphical view]
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3HAO_BURPP

Accession

Primary (citable) accession number: B2T2S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)