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B2T2E3

- PNP_BURPP

UniProt

B2T2E3 - PNP_BURPP

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, Bphyt_1339
Organism
Burkholderia phytofirmans (strain DSM 17436 / PsJN)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi493 – 4931Magnesium By similarity
Metal bindingi499 – 4991Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBPHY398527:GJEX-1345-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Bphyt_1339
OrganismiBurkholderia phytofirmans (strain DSM 17436 / PsJN)
Taxonomic identifieri398527 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001739: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_1000147898Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi398527.Bphyt_1339.

Structurei

3D structure databases

ProteinModelPortaliB2T2E3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini560 – 61960KHAdd
BLAST
Domaini629 – 69769S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiHGNFKSN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2T2E3-1 [UniParc]FASTAAdd to Basket

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MTMFNKIVKE FQWGQHKVRL ETGEIARQAS GAVIVDVEDT VVLATVVGAK    50
TAKPGQDFFP LTVDYLEKTY AAGKIPGGFF RREGRPSEGE TLISRLIDRP 100
LRPLFPEGFY NEVQVVIHVL SLNPEIPADI PALIGASAAL AVSGLPFNGP 150
VGAARVAYIN NEYVLNPTRP QMKESALDLI VAGTERAVLM VESEAQQLSE 200
EVMLGGVVFG HEQMQIAIDA IHDLVREGGK PEWDWQPAAK NEPLIARVTE 250
LAQADLLAAY QLRDKQARSA KLKEVYAATS KKLEEDAAAG GTVAADKATV 300
GNVLFDIEAK IVRTQILNGE PRIDGRDTRT VRPIEIRTGV LPRTHGSALF 350
TRGETQAMVV ATLGTKGDEQ NIDALEGEYR ERFMLHYNMP PFATGETGRV 400
GSPKRREIGH GRLAKRALAA CLPSADEFGY SIRVVSEITE SNGSSSMASV 450
CGGCLALMDA GVPMKAHVAG IAMGLILEGN KFAVLTDILG DEDHLGDMDF 500
KVAGTEQGVT ALQMDIKIQG ITKEIMQVAL AQAKEGRMHI LSKMTSAVSG 550
ANTVLSDYAP RMITIKINPE KIRDVIGKGG SVIRALTEET GTTIDISDDG 600
VVTIASTSSE GMAEAKKRIE NITLEVEVGQ VYEGTVLKLL DFGAIVNILP 650
GKDGLLHISE IANERIKDIN DYLKDGQQVK VKVIQTDEKG RVRLSAKALL 700
NEGANGASGA PQGEPTPQ 718
Length:718
Mass (Da):77,230
Last modified:July 1, 2008 - v1
Checksum:i36F7620DDCBB6045
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001052 Genomic DNA. Translation: ACD15754.1.
RefSeqiWP_012432372.1. NC_010681.1.
YP_001894978.1. NC_010681.1.

Genome annotation databases

EnsemblBacteriaiACD15754; ACD15754; Bphyt_1339.
GeneIDi6280971.
KEGGibpy:Bphyt_1339.
PATRICi19212724. VBIBurPhy117947_5148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001052 Genomic DNA. Translation: ACD15754.1 .
RefSeqi WP_012432372.1. NC_010681.1.
YP_001894978.1. NC_010681.1.

3D structure databases

ProteinModelPortali B2T2E3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 398527.Bphyt_1339.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD15754 ; ACD15754 ; Bphyt_1339 .
GeneIDi 6280971.
KEGGi bpy:Bphyt_1339.
PATRICi 19212724. VBIBurPhy117947_5148.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi HGNFKSN.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci BPHY398527:GJEX-1345-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.
    , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17436 / PsJN.

Entry informationi

Entry nameiPNP_BURPP
AccessioniPrimary (citable) accession number: B2T2E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: September 3, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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