B2T2A3 (HEM6_BURPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	Bphyt_1299

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000119794
Regions
	Region	55 – 64	10	Important for dimerization By similarity
	Region	115 – 117	3	Substrate binding By similarity
	Region	247 – 282	36	Important for dimerization By similarity
	Region	265 – 270	6	Substrate binding By similarity
Sites
	Active site	113	1	Proton donor By similarity
	Binding site	99	1	Substrate By similarity
	Site	182	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2T2A3 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 4893AA679713D09B	FASTA	307	34,585
	10 20 30 40 50 60 MTDSSYDAQA VRSWLQGLQT HIADTLGAFD GTPFATDAWQ RAPGEKLRGG GYTRILEGGK 70 80 90 100 110 120 FFERAGIGFS DVAGDALPGS ASAARPQLAG RGFEAMGVSL VLHPHNPHCP TVHMNVRLLI 130 140 150 160 170 180 ATKPGEEPVF WFGGGMDLTP YYGYEEDARH FHRTCRDALQ PYGADLYPSF KRWCDEYFFL 190 200 210 220 230 240 KHRNEARGIG GIFFDDFSAP GFDQSFAMLK SVGDAFLKAY LPIVEKRRNI PYGQAERDFQ 250 260 270 280 290 300 AYRRGRYVEF NLVFDRGTLF GLQSGGRTES ILMSMPPVVN WRYNWHPEPG TPEARLYSDF LVPREWV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17436 / PsJN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001052 Genomic DNA. Translation: ACD15714.1.
RefSeq	YP_001894938.1. NC_010681.1.
3D structure databases
ProteinModelPortal	B2T2A3.
SMR	B2T2A3. Positions 5-307.
ModBase	Search...
Protein-protein interaction databases
STRING	B2T2A3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6282963.
GenomeReviews	Gene locus Bphyt_1299 in contig CP001052_GR.
KEGG	bpy:Bphyt_1299.
PATRIC	19212636. VBIBurPhy117947_5104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG631180.
OMA	VKAYLLD.
ProtClustDB	PRK05330.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_BURPP

Accession

Primary (citable) accession number: B2T2A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program