ID   B2T1D0_PARPJ            Unreviewed;      1075 AA.
AC   B2T1D0;
DT   01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   01-JUL-2008, sequence version 1.
DT   27-MAR-2024, entry version 97.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
DE   Flags: Precursor;
GN   Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN   OrderedLocusNames=Bphyt_1068 {ECO:0000313|EMBL:ACD15487.1};
OS   Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)
OS   (Burkholderia phytofirmans).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=398527 {ECO:0000313|EMBL:ACD15487.1, ECO:0000313|Proteomes:UP000001739};
RN   [1] {ECO:0000313|EMBL:ACD15487.1, ECO:0000313|Proteomes:UP000001739}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17436 / LMG 22146 / PsJN
RC   {ECO:0000313|Proteomes:UP000001739};
RX   PubMed=21551308; DOI=10.1128/JB.05055-11;
RA   Weilharter A., Mitter B., Shin M.V., Chain P.S., Nowak J., Sessitsch A.;
RT   "Complete genome sequence of the plant growth-promoting endophyte
RT   Burkholderia phytofirmans strain PsJN.";
RL   J. Bacteriol. 193:3383-3384(2011).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; CP001052; ACD15487.1; -; Genomic_DNA.
DR   RefSeq; WP_012432115.1; NC_010681.1.
DR   AlphaFoldDB; B2T1D0; -.
DR   STRING; 398527.Bphyt_1068; -.
DR   KEGG; bpy:Bphyt_1068; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_4; -.
DR   OrthoDB; 9768133at2; -.
DR   Proteomes; UP000001739; Chromosome 1.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Pyruvate {ECO:0000313|EMBL:ACD15487.1}.
FT   REGION          1..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          120..148
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        33..47
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        122..137
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        285
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        727
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   1075 AA;  117477 MW;  F09767E7C26F0649 CRC64;
     MTSSGSARPA RRNTALPNAR AADAAQPAAS AIASAAASAT TAAESTSAKR AGKPAQAAHA
     AKAGKAAKTS TPVKTDKAVK AVKTGKTTQA AATVKAQKPA KAASAKIKVV ANAPQAPALQ
     AVAEQSPASA NTSAPKGNGR TREDKDHPLF QDIRYLGRLL GDVLREQEGD AVFDVVETIR
     QNAVRFRRED DSAAAQTLDK KLRSLSPEQT VSVVRAFSYF SHLANIAEDR HRNRRHRIHA
     LAGSTSQPGT IAHALERLVE ANAAATPVLQ QFFNDALIVP VLTAHPTEVQ RKSILDAQHD
     VARLLAERDQ QLTERERAHN EAMLRARVTS LWQTRMLRDS RLSVADEIEN ALSYYRATFL
     TEIPALYADI EEALTEHGID ARLPPFFQMG SWIGGDRDGN PNVTAETLEN AITRQAAVIF
     EHYMEQVHKL GAELSVSNLL AGASEALKEL AAISPDQSPH RTDEPYRRAL IGMYTRLAAS
     ARVRLGEGSV PVRSAGRGVA PIRAKPYDDS SEFVRDLHVL IDSLAEHHGA PLAAPRLSPL
     ARAAEVFGFH LASIDLRQSS DIHEAVIAEL LKRAGVEDDY AALTEEDKLK VLLAELAQPR
     SLRLPYAEYS DLVKSELGVL EEARITREKF GARAVRNYII SHTETVSDLV EVMLLQKETG
     LLRGRLGDAN DPAQAGLMVI PLFETIPDLR NAPHIMRDLI ALPGVDALIE HQGNEQEVML
     GYSDSNKDGG FLTSNWELYR AELALVSLFN ERGVTLRLFH GRGGTVGRGG GPTYQAILSQ
     PPGTVDGQIR LTEQGEVIAS KFGNPEIGRR NLETVVAATL EASLLPHDIA PAQLPAFEET
     MQQLSDAAMA SYRALVYETP GFKEYFFEST PIAEIAELNI GSRPASRKLQ DPKQRKIEDL
     RAIPWGFSWG QCRLLLTGWY GFGSAVATYL DSAPTDAERK SRLALLKKMH KTWPFFANLL
     SNMDMVLAKT DLAVASRYAA LVSDKKLRKH VFERIVAEWE RTSKVLSEIT GKSERLAENP
     LLARSIKNRF PYLDPLNHLQ VELLKRHRAG DTNARVRRGI HLSINGIAAG LRNTG
//