Reviewed, UniProtKB/Swiss-Prot B2T146 (AMPA_BURPP)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Bphyt_0984

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000098311
Sites
	Active site	286	1	Potential
	Active site	360	1	Potential
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	279	1	Manganese 1 By similarity
	Metal binding	279	1	Manganese 2 By similarity
	Metal binding	297	1	Manganese 2 By similarity
	Metal binding	356	1	Manganese 1 By similarity
	Metal binding	358	1	Manganese 1 By similarity
	Metal binding	358	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2T146-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 3EECF80517C646DA	FASTA	508	53,489
	10 20 30 40 50 60 MDFSIKACDW TKGSSNGFLT GKSDCIVIGV FESQTLSGAA LEIDEATRGL LTRIIKAGDM 70 80 90 100 110 120 DGKAGTTLFL HEVSGIGASR VLLVGLGKQD AFNQKAYGDA VRAAWRALLG TKIVQVTFTL 130 140 150 160 170 180 AQAPILERTA DWGVRAAILA LRELTYKFTQ MKSKPENAAR ALKRIVFSVN TGDEKAAKLA 190 200 210 220 230 240 AKQGAALANG MDLTRDLGNL PSNVCTPTYL ANTAKKLAKD WKLKVEVLGE KQCEALKMGS 250 260 270 280 290 300 FLSVTAGSVE PAQFIVLQYQ GGAAKAAPVV LVGKGVTFDT GGISLKPGEG MDEMKYDMCG 310 320 330 340 350 360 AGSVLGTLRA VAEMGLKLNV VGIIPAVENM PSATATKPGD IVTSMKGLTI EVLNTDAEGR 370 380 390 400 410 420 LILCDALTYA ERFKPAAVID IATLTGACII ALGHHNSGLF SKDDALAGEL LDASREASDP 430 440 450 460 470 480 AWRLPLDDEY QDQLKSNFAD LANIGGRPAG SVTAACFLSR FTDAYPWAHL DIAGTAWKSG 490 500 AAKGATGRPV PLLAQFLIDR AADGRATQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001052 Genomic DNA. Translation: ACD15403.1.
RefSeq	YP_001894627.1.
3D structure databases
SMR	B2T146. Positions 19-503.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	6284180.
GenomeReviews	Gene locus Bphyt_0984 in contig CP001052_GR.
KEGG	bpy:Bphyt_0984.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	LGHHNSG.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BURPP

Accession

Primary (citable) accession number: B2T146

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 19, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)