B2T067 (PUR5_BURPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	Bphyt_0748

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_1000193006

Sequences

	Sequence		Length	Mass (Da)
	B2T067 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 10EEA9579611109D	FASTA	355	36,946
	10 20 30 40 50 60 MNQPKSAPNS TDSAQGLSYR DAGVDIDAGD ALVDAIKPFA KKTMRDGVLG GIGGFGALFE 70 80 90 100 110 120 VPKKYKEPVL VSGTDGVGTK LRLAFQLNKH DTVGQDLVAM SVNDILVQGA EPLFFLDYFA 130 140 150 160 170 180 CGKLDVGTAA TVVKGIAHGC ELSGCALIGG ETAEMPGMYP DGEYDLAGFA VGAVEKSKII 190 200 210 220 230 240 DGSTIAPGDV VLGLASSGIH SNGFSLVRKI IERAQPDLNA DFDGRSLADA LMAPTHIYVK 250 260 270 280 290 300 PLLALMQQIA VKGMAHITGG GLVENIPRVL REGLTAELDH RGWPLPPLFS WLQKHGGVAD 310 320 330 340 350 AEMHRVFNCG IGMAVVVSAA DADAAIGLLS AAGEQVWKIG VIRESAAGEA QTVVV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17436 / PsJN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001052 Genomic DNA. Translation: ACD15172.1.
RefSeq	YP_001894396.1. NC_010681.1.
3D structure databases
ProteinModelPortal	B2T067.
SMR	B2T067. Positions 17-355.
ModBase	Search...
Protein-protein interaction databases
STRING	B2T067.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6281168.
GenomeReviews	Gene locus Bphyt_0748 in contig CP001052_GR.
KEGG	bpy:Bphyt_0748.
PATRIC	19211429. VBIBurPhy117947_4503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG531222.
OMA	GIDMIAM.
ProtClustDB	PRK05385.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BURPP

Accession

Primary (citable) accession number: B2T067

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program