B2SYX8 (MURD_BURPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Bphyt_3472

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130836
Regions
	Nucleotide binding	132 – 138	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B2SYX8 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: DDCCDA75B16347CC	FASTA	504	53,679
	10 20 30 40 50 60 MFGEKFRDRQ KPMVLVLGLG ESGLAMARWC ARHGCRLRVA DTREVPPNLP ALEAHGIDAA 70 80 90 100 110 120 FVGGPFSPVL LEGVELVAIS PGLSPLAADL VPLISAAREQ GIPVWGELEL FSQALKALGE 130 140 150 160 170 180 SGYAPKVIAI TGTNGKTTTT SLTGLLCERA GKKVAVAGNI SPALLDKLSE AIDNTALPDV 190 200 210 220 230 240 WVLELSSFQL ETAHTFTPDA AVVLNITQDH LDWHGGLDAY AAAKGRIFGA QTVRVLNRDD 250 260 270 280 290 300 SRVMKLAPAE DSEAEMVTFG VTEPKNDGDY GLLRDNGMIW LVQAHDRDAT DEPVPKRRRK 310 320 330 340 350 360 NEVVTPPNIA LKRLMPADAL RIRGLHNAAN ALAAYALTRA IGLPGAPLLH GLREYRGEPH 370 380 390 400 410 420 RVELIASIDG IDYVDDSKGT NVGATVAALD GLAQRIVLIA GGDGKGQDFE PLAAPVMRWC 430 440 450 460 470 480 RAVMLIGRDA PLIRAALEDT GIAMTDHATL EEATRAATAL AEPGDAVLLS PACASFDMFK 490 500 GYAHRAAVFR STVEDIAAER GTMI

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17436 / PsJN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001052 Genomic DNA. Translation: ACD17862.1.
RefSeq	YP_001897086.1. NC_010681.1.
3D structure databases
ProteinModelPortal	B2SYX8.
ModBase	Search...
Protein-protein interaction databases
STRING	B2SYX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6282831.
GenomeReviews	Gene locus Bphyt_3472 in contig CP001052_GR.
KEGG	bpy:Bphyt_3472.
PATRIC	19217502. VBIBurPhy117947_7497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	PRK02006.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BURPP

Accession

Primary (citable) accession number: B2SYX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program