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Protein

Argininosuccinate synthase

Gene

argG

Organism
Burkholderia phytofirmans (strain DSM 17436 / PsJN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei90 – 901CitrullineUniRule annotation
Binding sitei95 – 951CitrullineUniRule annotation
Binding sitei120 – 1201ATP; via amide nitrogenUniRule annotation
Binding sitei122 – 1221AspartateUniRule annotation
Binding sitei126 – 1261AspartateUniRule annotation
Binding sitei126 – 1261CitrullineUniRule annotation
Binding sitei127 – 1271AspartateUniRule annotation
Binding sitei130 – 1301CitrullineUniRule annotation
Binding sitei182 – 1821CitrullineUniRule annotation
Binding sitei191 – 1911CitrullineUniRule annotation
Binding sitei267 – 2671CitrullineUniRule annotation
Binding sitei279 – 2791CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPUniRule annotation

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPHY398527:GJEX-3251-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Bphyt_3206
OrganismiBurkholderia phytofirmans (strain DSM 17436 / PsJN)
Taxonomic identifieri398527 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001739 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Argininosuccinate synthasePRO_1000089025Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi398527.Bphyt_3206.

Structurei

3D structure databases

ProteinModelPortaliB2SY63.
SMRiB2SY63. Positions 6-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2SY63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIKKVVLA YSGGLDTSVI LKWLQDNYDA EVVTFTADIG QGEELEPARK
60 70 80 90 100
KALQLGIKQD NIFIEDLREE FVRDFVFPMF RANTIYEGEY LLGTSIARPL
110 120 130 140 150
IAKRQIEIAR ATGAQAVSHG ATGKGNDQVR FELGYYALEP GIKVIAPWRE
160 170 180 190 200
WDLLSREKLL AYAEKAGIPI EMKHKQGGAP YSMDANLLHI SFEGRHLEDP
210 220 230 240 250
KAEAEADMWR WTVSPEQAPD QAEYIDIEYE HGDPVAINGK RLSAAEMLTE
260 270 280 290 300
LNRLGGKHGI GRLDLVENRY VGMKSRGCYE TPGGTIMLKA HRGIESITLD
310 320 330 340 350
REVAHLKDDL MARYASLIYN GYWWSPERRA IQVLIDHTQE KVNGWVRVKL
360 370 380 390 400
YKGSVSVVAR DSKETLFDKT IATFDDDGGA YNQADAGGFI KLNALRMRIA

ENARRQRG
Length:408
Mass (Da):45,923
Last modified:June 30, 2008 - v1
Checksum:i8E0598C8DBE03B96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001052 Genomic DNA. Translation: ACD17598.1.
RefSeqiWP_012434168.1. NC_010681.1.
YP_001896822.1. NC_010681.1.

Genome annotation databases

EnsemblBacteriaiACD17598; ACD17598; Bphyt_3206.
KEGGibpy:Bphyt_3206.
PATRICi19216914. VBIBurPhy117947_7205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001052 Genomic DNA. Translation: ACD17598.1.
RefSeqiWP_012434168.1. NC_010681.1.
YP_001896822.1. NC_010681.1.

3D structure databases

ProteinModelPortaliB2SY63.
SMRiB2SY63. Positions 6-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398527.Bphyt_3206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD17598; ACD17598; Bphyt_3206.
KEGGibpy:Bphyt_3206.
PATRICi19216914. VBIBurPhy117947_7205.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciBPHY398527:GJEX-3251-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.
    , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17436 / PsJN.

Entry informationi

Entry nameiASSY_BURPP
AccessioniPrimary (citable) accession number: B2SY63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: June 30, 2008
Last modified: March 31, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.