Reviewed, UniProtKB/Swiss-Prot B2SXB8 (HEMH_BURPP)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	Bphyt_0733

Organism

Burkholderia phytofirmans (strain DSM 17436 / PsJN) [Complete proteome] [HAMAP]

Taxonomic identifier

398527 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX By similarity. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Ferrochelatase HAMAP MF_00323		PRO_1000116035
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	295	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2SXB8-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: A7B9A270E837191A	FASTA	356	39,748
	10 20 30 40 50 60 MRFDLERPSQ NASSHRVAVL LINLGTPDAP TPRAVRRYLA QFLSDPRVVE IPALLWQIIL 70 80 90 100 110 120 RLLILPFRGV ASAKKYAAVW MPEGSPLRVN TEKQVEGLRH LLQLNDYTVL VDYAMRYGTP 130 140 150 160 170 180 GIPAMLNQLK LAGAERVLLM PMYPQYSSST TATAFDDAFS ALKRMRNQPE IRTVRQYADH 190 200 210 220 230 240 PAYIAALAAQ VHNYWHQHGR PDFAAGDKLV LSFHGVPKRT LDLGDPYHEQ CQQTGALLMQ 250 260 270 280 290 300 ALELTQVECR ITFQSRFGKA EWLQPYTAPT LKELGAAGVR RADVFCPGFT ADCLETIEEI 310 320 330 340 350 GMEVRDEFLH AGGKDFHRIP CLNASQAWIA ALGEIVAQNL QGWPVQALPV PHTTGA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phytofirmans PsJN." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Nowak J., Sessitsch A., Lazarovits G., Compant S., Barka E., Tiedje J. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001052 Genomic DNA. Translation: ACD15157.1.
RefSeq	YP_001894381.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6282091.
GenomeReviews	Gene locus Bphyt_0733 in contig CP001052_GR.
KEGG	bpy:Bphyt_0733.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697135.
OMA	TIEEIGM.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BURPP

Accession

Primary (citable) accession number: B2SXB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)