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Protein

NAD kinase

Gene

nadK

Organism
Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) (Burkholderia phytofirmans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75Proton acceptorUniRule annotation1
Binding sitei177NADUniRule annotation1
Binding sitei179NADUniRule annotation1
Binding sitei214NAD; via carbonyl oxygenUniRule annotation1
Binding sitei248NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 76NADUniRule annotation2
Nucleotide bindingi149 – 150NADUniRule annotation2
Nucleotide bindingi190 – 195NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Bphyt_0731
OrganismiParaburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) (Burkholderia phytofirmans)
Taxonomic identifieri398527 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001739 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001208371 – 300NAD kinaseAdd BLAST300

Proteomic databases

PRIDEiB2SXB6

Interactioni

Protein-protein interaction databases

STRINGi398527.Bphyt_0731

Structurei

3D structure databases

ProteinModelPortaliB2SXB6
SMRiB2SXB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F91 Bacteria
COG0061 LUCA
HOGENOMiHOG000227221
KOiK00858
OMAiVNLGHVG
OrthoDBiPOG091H02D1

Family and domain databases

Gene3Di2.60.200.30, 1 hit
3.40.50.10330, 2 hits
HAMAPiMF_00361 NAD_kinase, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR017437 ATP-NAD_kinase_PpnK-typ_C
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002504 NADK
PANTHERiPTHR20275 PTHR20275, 1 hit
PfamiView protein in Pfam
PF01513 NAD_kinase, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit

Sequencei

Sequence statusi: Complete.

B2SXB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVTSQFKTV ALVGRNNTPG IAEPLTALAS CIAKRGFEVV FEADTAAEIG
60 70 80 90 100
VTDYPALRPA EIGARADVAV VLGGDGTMLG IGRQLAPYRT PLIGINHGRL
110 120 130 140 150
GFITDIPISD MREIVPQMLS GNFEREERVL LEARIMRGGN PIYHALAFND
160 170 180 190 200
VVVNRSGFSG MAELHVSVDG RFMYNQRSDG LIVATPTGST AYALSSQGPI
210 220 230 240 250
LHPQLQGIVL VPIAPHALSN RPIVLPDDSK VSIQIVSGRE VNVNFDMQSF
260 270 280 290 300
TSLELGDTIE VRRSRHTVPM LHPVGYSFFT TLRKKLHWNE YPSHEEDSKP
Length:300
Mass (Da):32,739
Last modified:July 1, 2008 - v1
Checksum:i298D901CEA3F087F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001052 Genomic DNA Translation: ACD15155.1
RefSeqiWP_012431791.1, NC_010681.1

Genome annotation databases

EnsemblBacteriaiACD15155; ACD15155; Bphyt_0731
KEGGibpy:Bphyt_0731

Similar proteinsi

Entry informationi

Entry nameiNADK_PARPJ
AccessioniPrimary (citable) accession number: B2SXB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: May 23, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health