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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Xanthomonas oryzae pv. oryzae (strain PXO99A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi116Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi190Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi194Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi197Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciXORY360094:G1GBE-1868-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:PXO_00446
OrganismiXanthomonas oryzae pv. oryzae (strain PXO99A)
Taxonomic identifieri360094 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000001740 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003746491 – 484tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST484

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2SW86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 153MTTase N-terminalUniRule annotationAdd BLAST118
Domaini416 – 479TRAMUniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224767
KOiK06168

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

B2SW86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTSVSDLS TATAVDAPAL LPLPVARPSA PAVVRGKLYI KTHGCQMNEY
60 70 80 90 100
DSAKMADVLA ASEGLELTDN PEEADVVLVN TCSIREKAQE KVFSQLGRWK
110 120 130 140 150
ALKAGGKPVI IGVGGCVASQ EGEAIVKRAP YVDLVFGPQT LHRLPELIRA
160 170 180 190 200
RRESGKSQVD ISFPEIEKFD RLPEPRAEGP SAFVSIMEGC SKYCSFCVVP
210 220 230 240 250
YTRGEEVSRP FEDVLVEVAQ LAAQGVREIN LLGQNVNAYR GAYGADAGDP
260 270 280 290 300
AQYADLGLLI RTIAQIEGIG RIRFTTSHPL EFSDSLVDAY RDVPQLANCL
310 320 330 340 350
HLPVQAGSDR ILSAMKRGYT ALEFKSRIRK LRAVRPDISI SSDFIVGFPG
360 370 380 390 400
ETEADFEKTM KLIEDVGFDQ SFSFVYSRRP GTPASDLQDD TPETVKQARL
410 420 430 440 450
ARLQAHISAH AASISQSMVG SVQRVLVEGP SRRDPNELTG KSENMRPVNF
460 470 480
PGNPRLIGQF VDVLITEAMS NSLRGRIQLD DSAH
Length:484
Mass (Da):52,683
Last modified:May 5, 2009 - v2
Checksum:iCEEEC72BC07B9192
GO

Sequence cautioni

The sequence ACD58519 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000967 Genomic DNA Translation: ACD58519.1 Different initiation.
RefSeqiWP_041182646.1, NC_010717.2

Genome annotation databases

EnsemblBacteriaiACD58519; ACD58519; PXO_00446
GeneIDi34178682
KEGGixop:PXO_00446

Similar proteinsi

Entry informationi

Entry nameiMIAB_XANOP
AccessioniPrimary (citable) accession number: B2SW86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: March 28, 2018
This is version 61 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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