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B2SW86

- MIAB_XANOP

UniProt

B2SW86 - MIAB_XANOP

Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Xanthomonas oryzae pv. oryzae (strain PXO99A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

    Catalytic activityi

    N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi116 – 1161Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi190 – 1901Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi194 – 1941Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi197 – 1971Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. transferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciXORY360094:GI45-1890-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
    Alternative name(s):
    (Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
    tRNA-i(6)A37 methylthiotransferaseUniRule annotation
    Gene namesi
    Name:miaBUniRule annotation
    Ordered Locus Names:PXO_00446
    OrganismiXanthomonas oryzae pv. oryzae (strain PXO99A)
    Taxonomic identifieri360094 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
    ProteomesiUP000001740: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 484484tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000374649Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi360094.PXO_00446.

    Structurei

    3D structure databases

    ProteinModelPortaliB2SW86.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 153118MTTase N-terminalUniRule annotationAdd
    BLAST
    Domaini416 – 47964TRAMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
    Contains 1 MTTase N-terminal domain.UniRule annotation
    Contains 1 TRAM domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0621.
    HOGENOMiHOG000224767.
    KOiK06168.
    OrthoDBiEOG6P5ZD8.

    Family and domain databases

    Gene3Di3.80.30.20. 1 hit.
    HAMAPiMF_01864. tRNA_metthiotr_MiaB.
    InterProiIPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view]
    PANTHERiPTHR11918. PTHR11918. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
    PROSITEiPS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2SW86-1 [UniParc]FASTAAdd to Basket

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    MPGTSVSDLS TATAVDAPAL LPLPVARPSA PAVVRGKLYI KTHGCQMNEY    50
    DSAKMADVLA ASEGLELTDN PEEADVVLVN TCSIREKAQE KVFSQLGRWK 100
    ALKAGGKPVI IGVGGCVASQ EGEAIVKRAP YVDLVFGPQT LHRLPELIRA 150
    RRESGKSQVD ISFPEIEKFD RLPEPRAEGP SAFVSIMEGC SKYCSFCVVP 200
    YTRGEEVSRP FEDVLVEVAQ LAAQGVREIN LLGQNVNAYR GAYGADAGDP 250
    AQYADLGLLI RTIAQIEGIG RIRFTTSHPL EFSDSLVDAY RDVPQLANCL 300
    HLPVQAGSDR ILSAMKRGYT ALEFKSRIRK LRAVRPDISI SSDFIVGFPG 350
    ETEADFEKTM KLIEDVGFDQ SFSFVYSRRP GTPASDLQDD TPETVKQARL 400
    ARLQAHISAH AASISQSMVG SVQRVLVEGP SRRDPNELTG KSENMRPVNF 450
    PGNPRLIGQF VDVLITEAMS NSLRGRIQLD DSAH 484
    Length:484
    Mass (Da):52,683
    Last modified:May 5, 2009 - v2
    Checksum:iCEEEC72BC07B9192
    GO

    Sequence cautioni

    The sequence ACD58519.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000967 Genomic DNA. Translation: ACD58519.1. Different initiation.
    RefSeqiYP_001913051.1. NC_010717.1.

    Genome annotation databases

    EnsemblBacteriaiACD58519; ACD58519; PXO_00446.
    GeneIDi6305409.
    KEGGixop:PXO_00446.
    PATRICi24123160. VBIXanOry73153_1951.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000967 Genomic DNA. Translation: ACD58519.1 . Different initiation.
    RefSeqi YP_001913051.1. NC_010717.1.

    3D structure databases

    ProteinModelPortali B2SW86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 360094.PXO_00446.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD58519 ; ACD58519 ; PXO_00446 .
    GeneIDi 6305409.
    KEGGi xop:PXO_00446.
    PATRICi 24123160. VBIXanOry73153_1951.

    Phylogenomic databases

    eggNOGi COG0621.
    HOGENOMi HOG000224767.
    KOi K06168.
    OrthoDBi EOG6P5ZD8.

    Enzyme and pathway databases

    BioCyci XORY360094:GI45-1890-MONOMER.

    Family and domain databases

    Gene3Di 3.80.30.20. 1 hit.
    HAMAPi MF_01864. tRNA_metthiotr_MiaB.
    InterProi IPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view ]
    PANTHERi PTHR11918. PTHR11918. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
    PROSITEi PS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PXO99A.

    Entry informationi

    Entry nameiMIAB_XANOP
    AccessioniPrimary (citable) accession number: B2SW86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 45 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3