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B2SW86

- MIAB_XANOP

UniProt

B2SW86 - MIAB_XANOP

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Protein
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Gene
miaB, PXO_00446
Organism
Xanthomonas oryzae pv. oryzae (strain PXO99A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Iron-sulfur (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S) By similarity
Metal bindingi116 – 1161Iron-sulfur (4Fe-4S) By similarity
Metal bindingi190 – 1901Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi194 – 1941Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi197 – 1971Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciXORY360094:GI45-1890-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (EC:2.8.4.3)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene namesi
Name:miaB
Ordered Locus Names:PXO_00446
OrganismiXanthomonas oryzae pv. oryzae (strain PXO99A)
Taxonomic identifieri360094 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001740: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation
PRO_0000374649Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi360094.PXO_00446.

Structurei

3D structure databases

ProteinModelPortaliB2SW86.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 153118MTTase N-terminal
Add
BLAST
Domaini416 – 47964TRAM
Add
BLAST

Sequence similaritiesi

Contains 1 TRAM domain.

Phylogenomic databases

eggNOGiCOG0621.
HOGENOMiHOG000224767.
KOiK06168.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2SW86-1 [UniParc]FASTAAdd to Basket

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MPGTSVSDLS TATAVDAPAL LPLPVARPSA PAVVRGKLYI KTHGCQMNEY    50
DSAKMADVLA ASEGLELTDN PEEADVVLVN TCSIREKAQE KVFSQLGRWK 100
ALKAGGKPVI IGVGGCVASQ EGEAIVKRAP YVDLVFGPQT LHRLPELIRA 150
RRESGKSQVD ISFPEIEKFD RLPEPRAEGP SAFVSIMEGC SKYCSFCVVP 200
YTRGEEVSRP FEDVLVEVAQ LAAQGVREIN LLGQNVNAYR GAYGADAGDP 250
AQYADLGLLI RTIAQIEGIG RIRFTTSHPL EFSDSLVDAY RDVPQLANCL 300
HLPVQAGSDR ILSAMKRGYT ALEFKSRIRK LRAVRPDISI SSDFIVGFPG 350
ETEADFEKTM KLIEDVGFDQ SFSFVYSRRP GTPASDLQDD TPETVKQARL 400
ARLQAHISAH AASISQSMVG SVQRVLVEGP SRRDPNELTG KSENMRPVNF 450
PGNPRLIGQF VDVLITEAMS NSLRGRIQLD DSAH 484
Length:484
Mass (Da):52,683
Last modified:May 5, 2009 - v2
Checksum:iCEEEC72BC07B9192
GO

Sequence cautioni

The sequence ACD58519.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000967 Genomic DNA. Translation: ACD58519.1. Different initiation.
RefSeqiYP_001913051.1. NC_010717.1.

Genome annotation databases

EnsemblBacteriaiACD58519; ACD58519; PXO_00446.
GeneIDi6305409.
KEGGixop:PXO_00446.
PATRICi24123160. VBIXanOry73153_1951.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000967 Genomic DNA. Translation: ACD58519.1 . Different initiation.
RefSeqi YP_001913051.1. NC_010717.1.

3D structure databases

ProteinModelPortali B2SW86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 360094.PXO_00446.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD58519 ; ACD58519 ; PXO_00446 .
GeneIDi 6305409.
KEGGi xop:PXO_00446.
PATRICi 24123160. VBIXanOry73153_1951.

Phylogenomic databases

eggNOGi COG0621.
HOGENOMi HOG000224767.
KOi K06168.
OrthoDBi EOG6P5ZD8.

Enzyme and pathway databases

BioCyci XORY360094:GI45-1890-MONOMER.

Family and domain databases

Gene3Di 3.80.30.20. 1 hit.
HAMAPi MF_01864. tRNA_metthiotr_MiaB.
InterProi IPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view ]
PANTHERi PTHR11918. PTHR11918. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
PROSITEi PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PXO99A.

Entry informationi

Entry nameiMIAB_XANOP
AccessioniPrimary (citable) accession number: B2SW86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: May 14, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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