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B2SW86 (MIAB_XANOP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB

EC=2.-.-.-
Alternative name(s):
tRNA-i(6)A37 methylthiotransferase
Gene names
Name:miaB
Ordered Locus Names:PXO_00446
OrganismXanthomonas oryzae pv. oryzae (strain PXO99A) [Complete proteome] [HAMAP]
Taxonomic identifier360094 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01864

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01864.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Sequence caution

The sequence ACD58519.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

transferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864
PRO_0000374649

Regions

Domain36 – 153118MTTase N-terminal
Domain416 – 47964TRAM

Sites

Metal binding451Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity
Metal binding1161Iron-sulfur (4Fe-4S) By similarity
Metal binding1901Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1941Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1971Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
B2SW86 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: CEEEC72BC07B9192

FASTA48452,683
        10         20         30         40         50         60 
MPGTSVSDLS TATAVDAPAL LPLPVARPSA PAVVRGKLYI KTHGCQMNEY DSAKMADVLA 

        70         80         90        100        110        120 
ASEGLELTDN PEEADVVLVN TCSIREKAQE KVFSQLGRWK ALKAGGKPVI IGVGGCVASQ 

       130        140        150        160        170        180 
EGEAIVKRAP YVDLVFGPQT LHRLPELIRA RRESGKSQVD ISFPEIEKFD RLPEPRAEGP 

       190        200        210        220        230        240 
SAFVSIMEGC SKYCSFCVVP YTRGEEVSRP FEDVLVEVAQ LAAQGVREIN LLGQNVNAYR 

       250        260        270        280        290        300 
GAYGADAGDP AQYADLGLLI RTIAQIEGIG RIRFTTSHPL EFSDSLVDAY RDVPQLANCL 

       310        320        330        340        350        360 
HLPVQAGSDR ILSAMKRGYT ALEFKSRIRK LRAVRPDISI SSDFIVGFPG ETEADFEKTM 

       370        380        390        400        410        420 
KLIEDVGFDQ SFSFVYSRRP GTPASDLQDD TPETVKQARL ARLQAHISAH AASISQSMVG 

       430        440        450        460        470        480 
SVQRVLVEGP SRRDPNELTG KSENMRPVNF PGNPRLIGQF VDVLITEAMS NSLRGRIQLD 


DSAH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000967 Genomic DNA. Translation: ACD58519.1. Different initiation.
RefSeqYP_001913051.1. NC_010717.1.

3D structure databases

ProteinModelPortalB2SW86.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360094.PXO_00446.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD58519; ACD58519; PXO_00446.
GeneID6305409.
KEGGxop:PXO_00446.
PATRIC24123160. VBIXanOry73153_1951.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0621.
HOGENOMHOG000224767.
KOK06168.
OrthoDBEOG6P5ZD8.
ProtClustDBPRK14325.

Enzyme and pathway databases

BioCycXORY360094:GI45-1890-MONOMER.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIAB_XANOP
AccessionPrimary (citable) accession number: B2SW86
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: February 19, 2014
This is version 43 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families