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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Xanthomonas oryzae pv. oryzae (strain PXO99A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

Zn2+UniRule annotation, Mg2+UniRule annotation, Co2+UniRule annotationNote: Binds 1 divalent metal cation per subunit. Can use ions such as Zn2+, Mg2+ or Co2+.UniRule annotation

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Phosphoserine aminotransferase (serC)
  4. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA)
  5. Pyridoxine 5'-phosphate synthase (pdxJ)
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132SubstrateUniRule annotation1
Metal bindingi160Divalent metal cation; shared with dimeric partnerUniRule annotation1
Metal bindingi205Divalent metal cation; shared with dimeric partnerUniRule annotation1
Metal bindingi260Divalent metal cation; shared with dimeric partnerUniRule annotation1
Binding sitei268SubstrateUniRule annotation1
Binding sitei277SubstrateUniRule annotation1
Binding sitei286SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandCobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Ordered Locus Names:PXO_04644
OrganismiXanthomonas oryzae pv. oryzae (strain PXO99A)
Taxonomic identifieri360094 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000001740 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001282661 – 3224-hydroxythreonine-4-phosphate dehydrogenaseAdd BLAST322

Proteomic databases

PRIDEiB2SPT4

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2SPT4
SMRiB2SPT4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000221592
KOiK00097
OMAiAPVHKGV

Family and domain databases

HAMAPiMF_00536 PdxA, 1 hit
InterProiView protein in InterPro
IPR037510 PdxA
IPR005255 PdxA_fam
PANTHERiPTHR30004 PTHR30004, 1 hit
PfamiView protein in Pfam
PF04166 PdxA, 1 hit
TIGRFAMsiTIGR00557 pdxA, 1 hit

Sequencei

Sequence statusi: Complete.

B2SPT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPSLALVPG EPAGIGPELC VRLAQRPRPD AHLIAYADPD TLHSAAKSLS
60 70 80 90 100
LSVRLLDPDQ PARAPGDLPL HPVRQAVATR FGAPDPANAA AVIAGLRGAA
110 120 130 140 150
DACLTGRLQG IVTGPVHKAV INAGGIPYTG TTELLADHAG CPVVMMLANS
160 170 180 190 200
IVRVALVTTH LPLRAVADAI TADALQQCLR ITHAAMQRDF GLEDPRIAVL
210 220 230 240 250
GLNPHAGEDG LLGREELDIV IPVIEQLRGD GMHLIGPLPA DTAFLPQKLG
260 270 280 290 300
GFDAVVAMYH DQGLPVLKYS GFEQAVNITL GLPYPRVAVD HGTALELAGR
310 320
RIADPSSLMA ATALCARLAA RR
Length:322
Mass (Da):33,603
Last modified:July 1, 2008 - v1
Checksum:i98B9ED66E1AFCD6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000967 Genomic DNA Translation: ACD57771.1

Genome annotation databases

EnsemblBacteriaiACD57771; ACD57771; PXO_04644
KEGGixop:PXO_04644

Similar proteinsi

Entry informationi

Entry nameiPDXA_XANOP
AccessioniPrimary (citable) accession number: B2SPT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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