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B2SH72

- LIPA_FRATM

UniProt

B2SH72 - LIPA_FRATM

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Protein

Lipoyl synthase

Gene
lipA, FTM_1205
Organism
Francisella tularensis subsp. mediasiatica (strain FSC147)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi102 – 1021Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi105 – 1051Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciFTUL441952:GIY0-1202-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:FTM_1205
OrganismiFrancisella tularensis subsp. mediasiatica (strain FSC147)
Taxonomic identifieri441952 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000001196: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Lipoyl synthaseUniRule annotationPRO_1000099604Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi441952.FTM_1205.

Structurei

3D structure databases

ProteinModelPortaliB2SH72.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiRKVDCDI.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B2SH72-1 [UniParc]FASTAAdd to Basket

« Hide

MKEISGIKVK VESGSKYTTD HGFHAVKDGI RNKKENAVHV RKPDWLKVQK    50
QDSKEYLKVK SITKKHKLST VCEEARCPNI NECWSHGTAT IMLMGSVCTR 100
ACKFCSVDTG NPKGWLDKDE PMNAAESVKL MGLEYVVLTS VDRDDLEDGG 150
AGHYAATITA IKNLDENIKV EALTPDFAGI NENIDKIINT KVDVIAQNIE 200
TVERLTHPVR DPRAGYWQTL NFLKYVKQKS PNVLTKTSIM VGLGETDEEI 250
YKTMDDARSV GIDIITLGQY MQPTKHHLSV ERFVTPQQFE EYRKVGLEKG 300
FLEVASGPMV RSSYRADRVF KRNNLDL 327
Length:327
Mass (Da):36,854
Last modified:July 1, 2008 - v1
Checksum:i5B9FC393926A76ED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000915 Genomic DNA. Translation: ACD31080.1.
RefSeqiWP_012429643.1. NC_010677.1.
YP_001891859.1. NC_010677.1.

Genome annotation databases

EnsemblBacteriaiACD31080; ACD31080; FTM_1205.
GeneIDi6290999.
KEGGiftm:FTM_1205.
PATRICi17957218. VBIFraTul67519_1495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000915 Genomic DNA. Translation: ACD31080.1 .
RefSeqi WP_012429643.1. NC_010677.1.
YP_001891859.1. NC_010677.1.

3D structure databases

ProteinModelPortali B2SH72.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 441952.FTM_1205.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD31080 ; ACD31080 ; FTM_1205 .
GeneIDi 6290999.
KEGGi ftm:FTM_1205.
PATRICi 17957218. VBIFraTul67519_1495.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi RKVDCDI.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci FTUL441952:GIY0-1202-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular evolutionary consequences of niche restriction in Francisella tularensis, a facultative intracellular pathogen."
    Larsson P., Elfsmark D., Svensson K., Wikstroem P., Forsman M., Brettin T., Keim P., Johansson A.
    PLoS Pathog. 5:E1000472-E1000472(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FSC147.

Entry informationi

Entry nameiLIPA_FRATM
AccessioniPrimary (citable) accession number: B2SH72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: September 3, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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