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B2SH69 (RUVC_FRATM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:FTM_1202
OrganismFrancisella tularensis subsp. mediasiatica (strain FSC147) [Complete proteome] [HAMAP]
Taxonomic identifier441952 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length171 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 171171Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000090528

Sites

Metal binding71Magnesium By similarity
Metal binding661Magnesium By similarity
Metal binding1381Magnesium By similarity
Metal binding1411Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B2SH69 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: B0F8E840F4C22DAD

FASTA17118,297
        10         20         30         40         50         60 
MVILGIDPGS RITGFGVIKV QDNKIYYVAS GCIRITEITT PKRLKQIADG ITQIINIYAP 

        70         80         90        100        110        120 
TEAAIEQIFM FQNPMGAIKL GQARGVAMCT LAINNLEVSE YSAKQIKQAV VGTGGAAKSQ 

       130        140        150        160        170 
VQHMVQSLLG LSKKPPEDAA DALAIAICHY HSSKSLAKIS GASRVSQKRI K 

« Hide

References

[1]"Molecular evolutionary consequences of niche restriction in Francisella tularensis, a facultative intracellular pathogen."
Larsson P., Elfsmark D., Svensson K., Wikstroem P., Forsman M., Brettin T., Keim P., Johansson A.
PLoS Pathog. 5:E1000472-E1000472(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FSC147.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000915 Genomic DNA. Translation: ACD31077.1.
RefSeqYP_001891856.1. NC_010677.1.

3D structure databases

ProteinModelPortalB2SH69.
SMRB2SH69. Positions 2-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING441952.FTM_1202.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD31077; ACD31077; FTM_1202.
GeneID6291171.
KEGGftm:FTM_1202.
PATRIC17957212. VBIFraTul67519_1492.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAMQIKLAL.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycFTUL441952:GIY0-1199-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_FRATM
AccessionPrimary (citable) accession number: B2SH69
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families