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B2SGM9 (SYL_FRATM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:FTM_0959
OrganismFrancisella tularensis subsp. mediasiatica (strain FSC147) [Complete proteome] [HAMAP]
Taxonomic identifier441952 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 813813Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091320

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif575 – 5795"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5781ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2SGM9 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 5AB1688851684D2E

FASTA81393,519
        10         20         30         40         50         60 
MNEYNFSDIE KSTQEYWRKN DTFKTIEDNT KEKFYCLSML PYPSGTLHMG HVRNYTIGDV 

        70         80         90        100        110        120 
IARYQKMQGK NVLHPMGWDA FGLPAENAAI KHKKSPYEWT KSNIAYMRSQ FDSLGFSFDW 

       130        140        150        160        170        180 
SREIATCDED YYKWEQWFFI QLYKKGLAYR KNSVVNWDPV DQTVLANEQV VDGRGWRSGA 

       190        200        210        220        230        240 
LVEKKEIPQW FLKITDYADE LLQDINKLDN WPEAVKTMQI NWIGKSKGLT VKFKVKDSNQ 

       250        260        270        280        290        300 
EIEVFTTRPD TLMGVNYLGI APEHPLALKE AKSNSQLAEF IEECKKTSTM EADLATQEKK 

       310        320        330        340        350        360 
GFKTSIKVIH PISAETIDVW VANFVLMGYG SGAVMSVPAH DQRDWEFAQK YNIPLKQVIE 

       370        380        390        400        410        420 
SNDNKLKIDL EKQAFTEKGI LINSGEFDSL NFKNAYQAIK KYLTEQNKGY ETTNFRIHDW 

       430        440        450        460        470        480 
GISRQRYWGC PIPMIHCDDC GAVPEKEENL PVRLPTDVAL TEAGSPLKDI PEFINVACPE 

       490        500        510        520        530        540 
CGKPAKRETD TFDTFFESSW YYARYTCPTA NQMLDQEANY WLPVDKYIGG IEHAIMHLLY 

       550        560        570        580        590        600 
ARFFHKLMRD QGLVKSDEPF KNLLTQGMVL KDGAKMSKSK GNIVDPQELI DKYGADTVRL 

       610        620        630        640        650        660 
FSMFAAPPEQ SLEWSETGVE GTNKFLRKVF NYAELNKVIF AKNITLESQK LTKEDKKARF 

       670        680        690        700        710        720 
EIHSNLKQAI FDFDKSQFNT VVSACMKILN TLNNYDNLSE SVKVEGFSIL LRILAPFTPH 

       730        740        750        760        770        780 
LCHYLWQQLN LGEDILHTSF PTVDNNALEK DEFLLVVQIN GKLKAKLELD ASLSSNQVEE 

       790        800        810 
VVLADEHVKS FIDNKQVVKV IYVPQKLINI VIK 

« Hide

References

[1]"Molecular evolutionary consequences of niche restriction in Francisella tularensis, a facultative intracellular pathogen."
Larsson P., Elfsmark D., Svensson K., Wikstroem P., Forsman M., Brettin T., Keim P., Johansson A.
PLoS Pathog. 5:E1000472-E1000472(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FSC147.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000915 Genomic DNA. Translation: ACD30888.1.
RefSeqYP_001891666.1. NC_010677.1.

3D structure databases

ProteinModelPortalB2SGM9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING441952.FTM_0959.

Proteomic databases

PRIDEB2SGM9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD30888; ACD30888; FTM_0959.
GeneID6290486.
KEGGftm:FTM_0959.
PATRIC17956608. VBIFraTul67519_1201.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycFTUL441952:GIY0-958-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_FRATM
AccessionPrimary (citable) accession number: B2SGM9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries