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B2SGE5

- GLYA_FRATM

UniProt

B2SGE5 - GLYA_FRATM

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Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Francisella tularensis subsp. mediasiatica (strain FSC147)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

Pyridoxal phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361Pyridoxal phosphateUniRule annotation
Binding sitei56 – 561Pyridoxal phosphateUniRule annotation
Binding sitei58 – 581SubstrateUniRule annotation
Binding sitei65 – 651SubstrateUniRule annotation
Binding sitei66 – 661Pyridoxal phosphateUniRule annotation
Binding sitei100 – 1001Pyridoxal phosphateUniRule annotation
Binding sitei122 – 1221Substrate; via carbonyl oxygenUniRule annotation
Binding sitei176 – 1761Pyridoxal phosphateUniRule annotation
Binding sitei204 – 2041Pyridoxal phosphateUniRule annotation
Binding sitei229 – 2291Pyridoxal phosphateUniRule annotation
Binding sitei236 – 2361Pyridoxal phosphateUniRule annotation
Binding sitei263 – 2631Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei363 – 3631Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciFTUL441952:GIY0-859-MONOMER.
UniPathwayiUPA00193.
UPA00288; UER01023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
Short name:
SHMTUniRule annotation
Short name:
Serine methylaseUniRule annotation
Gene namesi
Name:glyAUniRule annotation
Ordered Locus Names:FTM_0860
OrganismiFrancisella tularensis subsp. mediasiatica (strain FSC147)
Taxonomic identifieri441952 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000001196: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Serine hydroxymethyltransferasePRO_1000091542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei230 – 2301N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi441952.FTM_0860.

Structurei

3D structure databases

ProteinModelPortaliB2SGE5.
SMRiB2SGE5. Positions 8-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate bindingUniRule annotation
Regioni355 – 3573Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the SHMT family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0112.
HOGENOMiHOG000239404.
KOiK00600.
OMAiANMIELE.
OrthoDBiEOG6Z0QB2.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2SGE5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFSFEKNSLK NTDKEIFDAI ELEVKRQHEH VELIASENYA SPAVMEAQGS
60 70 80 90 100
QLTNKYAEGY HGKRYYGGCE FVDIAEKLAI ERAQQLFGVD YANVQPHSGS
110 120 130 140 150
QANAAVYNAV LKPGDTVLGM DLGAGGHLTH GSKVNFSGKI YNSIQYGLDE
160 170 180 190 200
NGDIDYEQVA QLAKEHKPKM IIAGFSAFSG IINWQKFREI ADSVDAVLMA
210 220 230 240 250
DIAHVAGLVA AGVYPNPFPY VYVATTTTHK TLRGPRGGLI LCNNNPELAK
260 270 280 290 300
KFQSAIFPGI QGGPLMHVIA AKAVAFKEAL EPSFVDYQKQ VLKNAKAMEK
310 320 330 340 350
VLKQRGINII SGGTSNHLLL LDITNTGFSG KEAEAALGRA NITVNKNSIP
360 370 380 390 400
NDPRSPFVTS GLRIGSPAIT TRGFKEKECE LVANLLADVV FNCGDEKVEN
410
ETAAKVLDLC DKFPVYK
Length:417
Mass (Da):45,317
Last modified:July 1, 2008 - v1
Checksum:i462C8B83E2E87B03
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000915 Genomic DNA. Translation: ACD30804.1.
RefSeqiYP_001891582.1. NC_010677.1.

Genome annotation databases

EnsemblBacteriaiACD30804; ACD30804; FTM_0860.
GeneIDi6290214.
KEGGiftm:FTM_0860.
PATRICi17956346. VBIFraTul67519_1072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000915 Genomic DNA. Translation: ACD30804.1 .
RefSeqi YP_001891582.1. NC_010677.1.

3D structure databases

ProteinModelPortali B2SGE5.
SMRi B2SGE5. Positions 8-416.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 441952.FTM_0860.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD30804 ; ACD30804 ; FTM_0860 .
GeneIDi 6290214.
KEGGi ftm:FTM_0860.
PATRICi 17956346. VBIFraTul67519_1072.

Phylogenomic databases

eggNOGi COG0112.
HOGENOMi HOG000239404.
KOi K00600.
OMAi ANMIELE.
OrthoDBi EOG6Z0QB2.

Enzyme and pathway databases

UniPathwayi UPA00193 .
UPA00288 ; UER01023 .
BioCyci FTUL441952:GIY0-859-MONOMER.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00051. SHMT.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view ]
PANTHERi PTHR11680. PTHR11680. 1 hit.
Pfami PF00464. SHMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000412. SHMT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00096. SHMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular evolutionary consequences of niche restriction in Francisella tularensis, a facultative intracellular pathogen."
    Larsson P., Elfsmark D., Svensson K., Wikstroem P., Forsman M., Brettin T., Keim P., Johansson A.
    PLoS Pathog. 5:E1000472-E1000472(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FSC147.

Entry informationi

Entry nameiGLYA_FRATM
AccessioniPrimary (citable) accession number: B2SGE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3