B2SFX3 (LPXB_FRATM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipid-A-disaccharide synthase
EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	FTM_0330

Organism

Francisella tularensis subsp. mediasiatica (strain FSC147) [Complete proteome] [HAMAP]

Taxonomic identifier

441952 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella tularensis subsp. mediasiatica

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00392
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP-Rule MF_00392
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP-Rule MF_00392
Sequence similarities	Belongs to the LpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Lipid-A-disaccharide synthase HAMAP-Rule MF_00392		PRO_1000191486

Sequences

	Sequence		Length	Mass (Da)
	B2SFX3 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 82D4B01962B0DF9C	FASTA	380	43,088
	10 20 30 40 50 60 MRIGIVAGEL SGDQLGGTLV EALKQKYPNA IIEGIGGPKM AAAGFKSLYP MDALSLIGFL 70 80 90 100 110 120 EIISKGLRIL SIRRKIINYF KQNKPDIFIG IDAPDFNLTV EKELRSAGIK TIHYVSPKIW 130 140 150 160 170 180 VWREYRIKKI RKATDKILAI LPFETEYYKN RHKFEAIYVG HPLAKNIPIH IDRAKYRDKL 190 200 210 220 230 240 GLKGSSLPIL SVLPGSRTTE VSRLLPLFLL ALQKLVDAGY KFKAIMPLAK PSLKPLFAKY 250 260 270 280 290 300 KEQIDSLGIE VFETNSHDVL KASDLSLLAS GTATLEAMLC KLPMVVGYKL SWLSALIGRM 310 320 330 340 350 360 LIGNHSYWAF PNILHKNEII KELIQEDCTV DNLFSELKRL FDDKRRNDYI VEEFEKIHKE 370 380 MVIDTESKII QVLDTMIEKS

References

[1]

"Molecular evolutionary consequences of niche restriction in Francisella tularensis, a facultative intracellular pathogen." Larsson P., Elfsmark D., Svensson K., Wikstroem P., Forsman M., Brettin T., Keim P., Johansson A. PLoS Pathog. 5:E1000472-E1000472(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FSC147.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000915 Genomic DNA. Translation: ACD30383.1.
RefSeq	YP_001891161.1. NC_010677.1.
3D structure databases
ProteinModelPortal	B2SFX3.
ModBase	Search...
Protein-protein interaction databases
STRING	441952.FTM_0330.
Protein family/group databases
CAZy	GT19. Glycosyltransferase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD30383; ACD30383; FTM_0330.
GeneID	6290459.
KEGG	ftm:FTM_0330.
PATRIC	17954998. VBIFraTul67519_0420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0763.
HOGENOM	HOG000018003.
KO	K00748.
OMA	PTVWAWR.
ProtClustDB	CLSK935090.
Enzyme and pathway databases
BioCyc	FTUL441952:GIY0-329-MONOMER.
UniPathway	UPA00359; UER00481.
Family and domain databases
HAMAP	MF_00392. LpxB.
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_FRATM

Accession

Primary (citable) accession number: B2SFX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program