Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Francisella tularensis subsp. mediasiatica (strain FSC147)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581Aminoacyl-adenylateUniRule annotation
Binding sitei602 – 6021ATPUniRule annotation
Metal bindingi897 – 8971ZincUniRule annotation
Metal bindingi900 – 9001ZincUniRule annotation
Metal bindingi917 – 9171ZincUniRule annotation
Metal bindingi920 – 9201ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFTUL441952:GIY0-502-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:FTM_0503
OrganismiFrancisella tularensis subsp. mediasiatica (strain FSC147)
Taxonomic identifieri441952 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 935935Isoleucine--tRNA ligasePRO_1000189167Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2SF88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi599 – 6035"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2SF88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKDTLNL PKTSFSMKGN LANKEPMILN KWEKQGIYKK IREHFAGREK
60 70 80 90 100
FILHDGPPYA NGSIHVGHAV NKILKDIIIK SKTLSGYDAP FTPTWDCHGL
110 120 130 140 150
PIELQVEKKH GKAGQSISED DFRKECRKYA KKQVEIQKKD FKRLGVLGDW
160 170 180 190 200
EQPYLTINFD YEANMIRTLA KIIENGHLSK GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYADKVSPAI DVKFKIKDKD KLAQAFGLDS LNHDAFAIIW TTTPWTLPAN
260 270 280 290 300
QAIAVNNQLN YSLIKIEDFY IILAENLVEQ TLKRYAIENA QIIATTTGNK
310 320 330 340 350
LTGIIAEHPF YSRHVPILHG DHVTDDSGTG LVHTAPTHGV DDFTLGKEHN
360 370 380 390 400
LSMEIFVKGN GCYSENTKLF AGEFIFKAND RIIELLGEKK RLMNSDKIKH
410 420 430 440 450
SYPHCWRHKT PLMFRATPQW FISMEKQGLR DKALQAIKET SWAPSWGQAR
460 470 480 490 500
IEGMVKDRPD WCISRQRTWG VPLSLFIHKE TEELHPNTIE ILYKVAEKIE
510 520 530 540 550
KDGIEAWFNA DDCEFITETA QYKSVKDTLD VWFDSGSSSM CILDLDKRLS
560 570 580 590 600
YPADLYLEGS DQHRGWFQTS LLVAMSAKGS QPYKEVFTHG FVVDEHGRKM
610 620 630 640 650
SKSLGNVTSP QDIYNTLGAD ILRLWTASTD YKSEMAVSDQ ILKRTADTYR
660 670 680 690 700
RLRNTARFLL SNLDGFNPVT DIIEFDKLVK LDQWAIAKTK EFQDKIIEAY
710 720 730 740 750
DKYQTHTVAQ LIHHFCSIEM GSFYLDIIKD RQYTAKTDGH PRKSAQTAIY
760 770 780 790 800
HIVHALVRWM APILSFTADE IWDATLKTTD LPIQLCEWYT GLKSFDQDAE
810 820 830 840 850
LDLEYWAKIQ EIRSEVNRVL EIKRNEDVIK ASLEAEITIY ADKYNYKLLE
860 870 880 890 900
KLGNELRFLL ISSKADLKVI EESTSSSIAA NIPGLLIEIT KIEEPKCERC
910 920 930
WHRSSTVGDN PQYKDICSRC VENITTEAGE SREFA
Length:935
Mass (Da):106,981
Last modified:July 1, 2008 - v1
Checksum:iC9CBE2D9F3CF49B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000915 Genomic DNA. Translation: ACD30520.1.
RefSeqiWP_012429277.1. NC_010677.1.
YP_001891298.1. NC_010677.1.

Genome annotation databases

EnsemblBacteriaiACD30520; ACD30520; FTM_0503.
KEGGiftm:FTM_0503.
PATRICi17955437. VBIFraTul67519_0634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000915 Genomic DNA. Translation: ACD30520.1.
RefSeqiWP_012429277.1. NC_010677.1.
YP_001891298.1. NC_010677.1.

3D structure databases

ProteinModelPortaliB2SF88.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD30520; ACD30520; FTM_0503.
KEGGiftm:FTM_0503.
PATRICi17955437. VBIFraTul67519_0634.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciFTUL441952:GIY0-502-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular evolutionary consequences of niche restriction in Francisella tularensis, a facultative intracellular pathogen."
    Larsson P., Elfsmark D., Svensson K., Wikstroem P., Forsman M., Brettin T., Keim P., Johansson A.
    PLoS Pathog. 5:E1000472-E1000472(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FSC147.

Entry informationi

Entry nameiSYI_FRATM
AccessioniPrimary (citable) accession number: B2SF88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: June 24, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.