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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Brucella abortus (strain S19)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotationSAAS annotation

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441NADUniRule annotation
Binding sitei45 – 451NADPUniRule annotation
Active sitei166 – 1661Proton donor/acceptorUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation
Active sitei170 – 1701Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 236NAD(P)UniRule annotation
Nucleotide bindingi108 – 1103NAD(P)UniRule annotation
Nucleotide bindingi132 – 1354NAD(P)UniRule annotation

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate reductase Source: UniProtKB-EC
  2. NAD binding Source: UniProtKB-HAMAP
  3. NADPH binding Source: InterPro
  4. oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotation

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

NADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBABO430066:GHI6-2982-MONOMER.
UniPathwayiUPA00034; UER00018.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotationSAAS annotation (EC:1.17.1.8UniRule annotationSAAS annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:BAbS19_II09380Imported
OrganismiBrucella abortus (strain S19)Imported
Taxonomic identifieri430066 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002565: Chromosome 2

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi430066.BAbS19_II09380.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni176 – 1772Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the DapB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0289.
HOGENOMiHOG000227153.
KOiK00215.
OMAiCAVYARE.
OrthoDBiEOG6SV5DS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR011770. Dihydrodipicolinate_Rdtase.
IPR023940. Dihydrodipicolinate_Rdtase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2SC36-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEEVQGGNS EMGLVVVGAG GRMGQTLIRT IQSIEGAKLV GAIERSGSPF
60 70 80 90 100
LGKDAGEVTG IGTLGVAITD DPLPVFAKAH GVLDFTSPAA SVEFAGLAAQ
110 120 130 140 150
ARIVHVIGTT GCSAEDDEKI RAAARHATIV KSGNMSLGVN LLSVLVQKAA
160 170 180 190 200
EALGPEDFDI EILEMHHRHK VDAPSGTALL LGEAAARGRD IALADNSVRV
210 220 230 240 250
RDGYTGPRET GAIGFATLRG GSVIGDHSVI LAGTGERVVL SHHAEDRSIF
260 270
ARGAIKAALW AHGKKPGLYS MLDVLGLNT
Length:279
Mass (Da):28,753
Last modified:July 1, 2008 - v1
Checksum:iC5F1BCA2389DF347
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000888 Genomic DNA. Translation: ACD74423.1.
RefSeqiYP_001932869.1. NC_010740.1.

Genome annotation databases

EnsemblBacteriaiACD74423; ACD74423; BAbS19_II09380.
GeneIDi6325885.
KEGGibmc:BAbS19_II09380.
PATRICi17816482. VBIBruAbo38055_1036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000888 Genomic DNA. Translation: ACD74423.1.
RefSeqiYP_001932869.1. NC_010740.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi430066.BAbS19_II09380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD74423; ACD74423; BAbS19_II09380.
GeneIDi6325885.
KEGGibmc:BAbS19_II09380.
PATRICi17816482. VBIBruAbo38055_1036.

Phylogenomic databases

eggNOGiCOG0289.
HOGENOMiHOG000227153.
KOiK00215.
OMAiCAVYARE.
OrthoDBiEOG6SV5DS.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018.
BioCyciBABO430066:GHI6-2982-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR011770. Dihydrodipicolinate_Rdtase.
IPR023940. Dihydrodipicolinate_Rdtase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes."
    Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W.
    PLoS ONE 3:E2193-E2193(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S19Imported.

Entry informationi

Entry nameiB2SC36_BRUA1
AccessioniPrimary (citable) accession number: B2SC36
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 2008
Last sequence update: July 1, 2008
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.