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B2SBF0

- BIOB_BRUA1

UniProt

B2SBF0 - BIOB_BRUA1

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Protein

Biotin synthase

Gene
bioB, BAbS19_II06920
Organism
Brucella abortus (strain S19)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi270 – 2701Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBABO430066:GHI6-2739-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:BAbS19_II06920
OrganismiBrucella abortus (strain S19)
Taxonomic identifieri430066 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002565: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Biotin synthaseUniRule annotationPRO_0000381247Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi430066.BAbS19_II06920.

Structurei

3D structure databases

ProteinModelPortaliB2SBF0.
SMRiB2SBF0. Positions 15-322.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B2SBF0-1 [UniParc]FASTAAdd to Basket

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MPDRGGENGA SCSVGRWSAE EARAIYNLPF NDLLFRAHGL HRENFDPNRI    50
QLSKLLNIKT GGCPEDCGYC SQSASAENGL KASKLMEIET VLEEARKAKA 100
AGATRYCMGA AWRSPKDRDM PALTHMIESV KAMGLETCMT LGMLDSDKAE 150
KLADAGLDYY NHNIDTSERF YPAVITTRSF EDRLDTLANV RNAGIKVCSG 200
GILGLGEEAE DRIDMLVTLA NLPEPPESVP INMLIPMPGT RLAKAAPVDP 250
LEFVRVVALA RILMPKSHVR LTAGRTAMSD EMQALCFFAG ANSLFMGDTL 300
LTAANPGDDR DSSLLRRLGI QAETEQPA 328
Length:328
Mass (Da):35,653
Last modified:July 28, 2009 - v2
Checksum:iB3936726D01DAB70
GO

Sequence cautioni

The sequence ACD74187.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000888 Genomic DNA. Translation: ACD74187.1. Different initiation.
RefSeqiYP_001932633.1. NC_010740.1.

Genome annotation databases

EnsemblBacteriaiACD74187; ACD74187; BAbS19_II06920.
GeneIDi6326128.
KEGGibmc:BAbS19_II06920.
PATRICi17815930. VBIBruAbo38055_0763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000888 Genomic DNA. Translation: ACD74187.1 . Different initiation.
RefSeqi YP_001932633.1. NC_010740.1.

3D structure databases

ProteinModelPortali B2SBF0.
SMRi B2SBF0. Positions 15-322.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 430066.BAbS19_II06920.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD74187 ; ACD74187 ; BAbS19_II06920 .
GeneIDi 6326128.
KEGGi bmc:BAbS19_II06920.
PATRICi 17815930. VBIBruAbo38055_0763.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci BABO430066:GHI6-2739-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes."
    Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W.
    PLoS ONE 3:E2193-E2193(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S19.

Entry informationi

Entry nameiBIOB_BRUA1
AccessioniPrimary (citable) accession number: B2SBF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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