B2SAD9 (RNC_BRUA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	BAbS19_I06380

Organism

Brucella abortus (strain S19) [Complete proteome] [HAMAP]

Taxonomic identifier

430066 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity. HAMAP MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity HAMAP MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro rRNA catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Ribonuclease 3 HAMAP MF_00104		PRO_1000094098
Regions
	Domain	19 – 144	126	RNase III
	Domain	169 – 238	70	DRBM

Sequences

	Sequence		Length	Mass (Da)
	B2SAD9 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 084ED2D3A3F16BFC	FASTA	245	27,220
	10 20 30 40 50 60 MNRTRPLPEI KMVSANKTAS ILEERTGHRF LNLKRLERAL THSSVQAPAR ANYERLEFLG 70 80 90 100 110 120 DRVLGLTVAE MLFEAFPEAS EGELSVRLNA LVNAETCAAI ADEIGLADLI HTGSDIKSLN 130 140 150 160 170 180 DKRLLNVRAD VVEALIATIY LDGGLEAARS FIQRYWKKRS LETGAARRDA KTELQEWAHQ 190 200 210 220 230 240 QGNVHPVYAI LSRSGPDHDP LFLVEVTVKG FAPEKGEGRS KRIAEQSAAE AMLYREGVWK RDGSA

References

[1]

"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes." Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W. PLoS ONE 3:E2193-E2193(2008) [PubMed: 18478107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S19.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000887 Genomic DNA. Translation: ACD72169.1.
RefSeq	YP_001934643.1. NC_010742.1.
3D structure databases
ProteinModelPortal	B2SAD9.
ModBase	Search...
Protein-protein interaction databases
STRING	B2SAD9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6327799.
GenomeReviews	Gene locus BAbS19_I06380 in contig CP000887_GR.
KEGG	bmc:BAbS19_I06380.
PATRIC	17818280. VBIBruAbo38055_1914.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG744556.
OMA	ALTHKSF.
ProtClustDB	PRK12371.
Family and domain databases
HAMAP	MF_00104. RNase_III. [Tree]
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
KO	K03685.
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_BRUA1

Accession

Primary (citable) accession number: B2SAD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program