B2S9Y5 (MTB1_BRUA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase BabI
Short name=M.BabI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase BabI
DNA methyltransferase CcrM
M.CcrMI ortholog

Gene names

Name:	ccrM
Ordered Locus Names:	BAbS19_I04800

Organism

Brucella abortus (strain S19) [Complete proteome] [HAMAP]

Taxonomic identifier

430066 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Modification methylase BabI		PRO_0000363188

Sequences

	Sequence		Length	Mass (Da)
	B2S9Y5 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 9D83AD96152CAD6C	FASTA	386	43,306
	10 20 30 40 50 60 MRSQVIEYPM SLVRLAHELP IEAPRTAWLD SIIKGDCVSA LERLPDHSVD VIFADPPYNL 70 80 90 100 110 120 QLGGDLHRPD QSMVSAVDDH WDQFESFQAY DAFTRAWLLA CRRVLKPNGT IWVIGSYHNI 130 140 150 160 170 180 FRVGTQLQDL GFWLLNDIVW RKTNPMPNFR GRRFQNAHET LIWASREQKG KGYTFNYEAM 190 200 210 220 230 240 KAANDDVQMR SDWLFPICTG SERLKDENGD KVHPTQKPEA LLARIMMASS KPGDVILDPF 250 260 270 280 290 300 FGSGTTGAVA KRLGRHFVGI EREQPYIDAA TARINAVEPL GKAELTVMTG KRAEPRVAFT 310 320 330 340 350 360 SVMEAGLLRP GTVLCDERRR FAAIVRADGT LTANGEAGSI HRIGARVQGF DACNGWTFWH 370 380 FEENGVLKPI DALRKIIREQ MAAAGA

References

[1]

"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes." Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W. PLoS ONE 3:E2193-E2193(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S19.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000887 Genomic DNA. Translation: ACD72015.1.
RefSeq	YP_001934489.1. NC_010742.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	430066.BAbS19_I04800.
Protein family/group databases
REBASE	18251. M.BabS19ORF4800P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD72015; ACD72015; BAbS19_I04800.
GeneID	6327641.
KEGG	bmc:BAbS19_I04800.
PATRIC	17817930. VBIBruAbo38055_1741.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0863.
HOGENOM	HOG000125329.
KO	K13581.
OMA	KVHSTQK.
ProtClustDB	CLSK2518600.
Enzyme and pathway databases
BioCyc	BABO430066:GHI6-472-MONOMER.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BRUA1

Accession

Primary (citable) accession number: B2S9Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 1, 2008
Last modified:	May 29, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program