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B2S9G0

- PNP_BRUA1

UniProt

B2S9G0 - PNP_BRUA1

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, BAbS19_I20300
Organism
Brucella abortus (strain S19)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881Magnesium By similarity
Metal bindingi494 – 4941Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBABO430066:GHI6-2039-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:BAbS19_I20300
OrganismiBrucella abortus (strain S19)
Taxonomic identifieri430066 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002565: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_1000147893Add
BLAST

Proteomic databases

PRIDEiB2S9G0.

Interactioni

Protein-protein interaction databases

STRINGi430066.BAbS19_I20300.

Structurei

3D structure databases

ProteinModelPortaliB2S9G0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61460KH
Add
BLAST
Domaini624 – 69269S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiHGNFKSN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2S9G0-1 [UniParc]FASTAAdd to Basket

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MFNTHKVEIE WGGRPLTLET GKIARQADGA VLATYGETAV LATVVSAKEP    50
KPGQDFFPLT VNYQEKTYAA GKIPGGYFKR EGRPSENETL VSRLIDRPIR 100
PLFVDGYKND TQVVITVLQH DLENNPDILS MVAASAALTI SGVPFMGPIS 150
GARVGYIDGE YVLNPNIDEM PESKLDLVVA GTSEAVLMVE SEAQELPEDV 200
MLGAVMFGHK SFQPVIDAII KLAEVAAKEP RDFQPEDLSE LEAKVLAVVE 250
NDLREAYKIT EKQARYAAVD AAKAKAKEHF FPEGVEETEM SAEQFATIFK 300
HLQAKIVRWN ILDTGNRIDG RDLSTVRPIV SEVGILPRTH GSALFTRGET 350
QAIVVATLGT GEDEQMIDAL TGTYKESFML HYNFPPYSVG ETGRMGSPGR 400
REIGHGKLAW RAIHPMLPAA EQFPYTIRAV SEITESNGSS SMATVCGTSL 450
ALMDAGVPIV RPVAGIAMGL IKEGERFAVL SDILGDEDHL GDMDFKVAGT 500
EFGITSLQMD IKIDGITEEI MKVALEQAKG GRVHILGEMA KAISSSRAEL 550
GEFAPRIEVM NIPTDKIRDV IGSGGKVIRE IVEKTGAKIN IEDDGTVKIA 600
SSNGKEIEAA KKWIHSIVAE PEVGEIYEGT VVKTADFGAF VNFFGPRDGL 650
VHISQLAADR VAKTTDVVKE GQKVWVKLMG FDERGKVRLS MKVVDQETGK 700
EIVAEKKKEE VDAE 714
Length:714
Mass (Da):77,730
Last modified:July 1, 2008 - v1
Checksum:i61E6696AD66B29F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000887 Genomic DNA. Translation: ACD73512.1.
RefSeqiYP_001935986.1. NC_010742.1.

Genome annotation databases

EnsemblBacteriaiACD73512; ACD73512; BAbS19_I20300.
GeneIDi6327590.
KEGGibmc:BAbS19_I20300.
PATRICi17821474. VBIBruAbo38055_3463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000887 Genomic DNA. Translation: ACD73512.1 .
RefSeqi YP_001935986.1. NC_010742.1.

3D structure databases

ProteinModelPortali B2S9G0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 430066.BAbS19_I20300.

Proteomic databases

PRIDEi B2S9G0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD73512 ; ACD73512 ; BAbS19_I20300 .
GeneIDi 6327590.
KEGGi bmc:BAbS19_I20300.
PATRICi 17821474. VBIBruAbo38055_3463.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi HGNFKSN.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci BABO430066:GHI6-2039-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes."
    Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W.
    PLoS ONE 3:E2193-E2193(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S19.

Entry informationi

Entry nameiPNP_BRUA1
AccessioniPrimary (citable) accession number: B2S9G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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