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Protein

Ribonuclease PH

Gene

rph

Organism
Brucella abortus (strain S19)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

GO - Molecular functioni

  1. tRNA binding Source: UniProtKB-HAMAP
  2. tRNA nucleotidyltransferase activity Source: UniProtKB-HAMAP
  3. tRNA-specific ribonuclease activity Source: InterPro

GO - Biological processi

  1. tRNA processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciBABO430066:GHI6-161-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Ordered Locus Names:BAbS19_I01630
OrganismiBrucella abortus (strain S19)
Taxonomic identifieri430066 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002565 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Ribonuclease PHPRO_1000129324Add
BLAST

Proteomic databases

PRIDEiB2S8G4.

Interactioni

Protein-protein interaction databases

STRINGi430066.BAbS19_I01630.

Structurei

3D structure databases

SMRiB2S8G4. Positions 2-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0689.
HOGENOMiHOG000229516.
KOiK00989.
OMAiGKGQGWV.
OrthoDBiEOG6CZQQP.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2S8G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPSKRAADE MRAISFERGV SKHAEGSCLV KFGDTHVLCT ASLEEKVPGW
60 70 80 90 100
MRNTGKGWVT AEYGMLPRST GERMRREAAA GKQGGRTQEI QRLIGRSLRA
110 120 130 140 150
VVDMQALGEM QITVDCDVIQ ADGGTRTAAI TGGWVALHEC LRWMEARQMV
160 170 180 190 200
RVEKVLKDHV AAISCGIYEG VPVLDLDYAE DSVAETDSNF VMTGKGGIVE
210 220 230
IQGTAEGVPF SEEEFGALMK LARSGIDRLV SLQKMAVA
Length:238
Mass (Da):25,907
Last modified:July 1, 2008 - v1
Checksum:i70CAF4B8F47A8E9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000887 Genomic DNA. Translation: ACD71717.1.
RefSeqiYP_001934191.1. NC_010742.1.

Genome annotation databases

EnsemblBacteriaiACD71717; ACD71717; BAbS19_I01630.
KEGGibmc:BAbS19_I01630.
PATRICi17817207. VBIBruAbo38055_1386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000887 Genomic DNA. Translation: ACD71717.1.
RefSeqiYP_001934191.1. NC_010742.1.

3D structure databases

SMRiB2S8G4. Positions 2-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi430066.BAbS19_I01630.

Proteomic databases

PRIDEiB2S8G4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD71717; ACD71717; BAbS19_I01630.
KEGGibmc:BAbS19_I01630.
PATRICi17817207. VBIBruAbo38055_1386.

Phylogenomic databases

eggNOGiCOG0689.
HOGENOMiHOG000229516.
KOiK00989.
OMAiGKGQGWV.
OrthoDBiEOG6CZQQP.

Enzyme and pathway databases

BioCyciBABO430066:GHI6-161-MONOMER.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes."
    Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W.
    PLoS ONE 3:E2193-E2193(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S19.

Entry informationi

Entry nameiRNPH_BRUA1
AccessioniPrimary (citable) accession number: B2S8G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: April 1, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.