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B2S8G4 (RNPH_BRUA1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease PH

Short name=RNase PH
EC=2.7.7.56
Alternative name(s):
tRNA nucleotidyltransferase
Gene names
Name:rph
Ordered Locus Names:BAbS19_I01630
OrganismBrucella abortus (strain S19) [Complete proteome] [HAMAP]
Taxonomic identifier430066 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564

Catalytic activity

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564

Sequence similarities

Belongs to the RNase PH family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiontRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA nucleotidyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

tRNA-specific ribonuclease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238Ribonuclease PH HAMAP-Rule MF_00564
PRO_1000129324

Sequences

Sequence LengthMass (Da)Tools
B2S8G4 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 70CAF4B8F47A8E9C

FASTA23825,907
        10         20         30         40         50         60 
MRPSKRAADE MRAISFERGV SKHAEGSCLV KFGDTHVLCT ASLEEKVPGW MRNTGKGWVT 

        70         80         90        100        110        120 
AEYGMLPRST GERMRREAAA GKQGGRTQEI QRLIGRSLRA VVDMQALGEM QITVDCDVIQ 

       130        140        150        160        170        180 
ADGGTRTAAI TGGWVALHEC LRWMEARQMV RVEKVLKDHV AAISCGIYEG VPVLDLDYAE 

       190        200        210        220        230 
DSVAETDSNF VMTGKGGIVE IQGTAEGVPF SEEEFGALMK LARSGIDRLV SLQKMAVA 

« Hide

References

[1]"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes."
Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W.
PLoS ONE 3:E2193-E2193(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S19.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000887 Genomic DNA. Translation: ACD71717.1.
RefSeqYP_001934191.1. NC_010742.1.

3D structure databases

ProteinModelPortalB2S8G4.
SMRB2S8G4. Positions 2-238.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING430066.BAbS19_I01630.

Proteomic databases

PRIDEB2S8G4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD71717; ACD71717; BAbS19_I01630.
GeneID6328108.
KEGGbmc:BAbS19_I01630.
PATRIC17817207. VBIBruAbo38055_1386.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0689.
HOGENOMHOG000229516.
KOK00989.
OMAICTAMVE.
OrthoDBEOG6CZQQP.

Enzyme and pathway databases

BioCycBABO430066:GHI6-161-MONOMER.

Family and domain databases

Gene3D3.30.230.70. 1 hit.
HAMAPMF_00564. RNase_PH.
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsTIGR01966. RNasePH. 1 hit.
PROSITEPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNPH_BRUA1
AccessionPrimary (citable) accession number: B2S8G4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families