B2S8E3 (LSPA_BRUA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BAbS19_I01400

Organism

Brucella abortus (strain S19) [Complete proteome] [HAMAP]

Taxonomic identifier

430066 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 160	160	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000097234
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	58 – 78	21	Helical; Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Transmembrane	127 – 147	21	Helical; Potential
Sites
	Active site	108	1	By similarity
	Active site	135	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2S8E3 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: D88410930B2A0B74	FASTA	160	18,230
	10 20 30 40 50 60 MKRHAVWSSL FVVILAVLID QGIKYLVESR MFYGQQIDLL PFLALFRTHN EGIAFSMLAW 70 80 90 100 110 120 LHDGGLIAIT LAVIAFVLYL WWTNAPERVF ARYGFALVIG GAIGNLIDRV MHGYVVDYVL 130 140 150 160 FHLPTWSFAV FNLADAFITI GAGLIILEEF LGWRRERISH

References

[1]

"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes." Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W. PLoS ONE 3:E2193-E2193(2008) [PubMed: 18478107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S19.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000887 Genomic DNA. Translation: ACD71696.1.
RefSeq	YP_001934170.1. NC_010742.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2S8E3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6328086.
GenomeReviews	Gene locus BAbS19_I01400 in contig CP000887_GR.
KEGG	bmc:BAbS19_I01400.
PATRIC	17817153. VBIBruAbo38055_1359.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724422.
OMA	SKFLILQ.
ProtClustDB	PRK14795.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BRUA1

Accession

Primary (citable) accession number: B2S8E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program