Reviewed, UniProtKB/Swiss-Prot B2S843 (GCP_BRUA1)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BAbS19_I17700

Organism

Brucella abortus (strain S19) [Complete proteome] [HAMAP]

Taxonomic identifier

430066 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc By similarity.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_1000145953
Sites
	Metal binding	115	1	Zinc Potential
	Metal binding	119	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	B2S843-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 78EB27EF0DCE9369	FASTA	359	38,013
	10 20 30 40 50 60 MRVLGIETSC DETAAAIVER DDMGEGRILS NVVLSQIAEH EPYGGVVPEI AARAHVEALD 70 80 90 100 110 120 RLVDRALNDA GLKLYEVDAV AATAGPGLIG GLIVGLMTAK ALAMAAQKPF YAVNHLEGHA 130 140 150 160 170 180 LTARLTDGLP FPYLLLLVSG GHTQMVLVRG IGDYERLGTT IDDALGEAFD KTAKLLGLPY 190 200 210 220 230 240 PGGPTVERMA LQGDQKRFAL PRPLKGEARL DFSFSGLKTA VRQTATELVP LTDQDVTDIC 250 260 270 280 290 300 ASFQAAVADT LSDRVGRSLE RFKTEFPDCA TPSLVVAGGV AANKTLRAAL ENLCTRHGFA 310 320 330 340 350 FIAPPLNLCT DNAAMIAWAG AERAATQAPD SLDIAPRSRW PLDEKSAPVF GTGRRGAKA

References

[1]

"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes." Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W. PLoS ONE 3:E2193-E2193(2008) [PubMed: 18478107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000887 Genomic DNA. Translation: ACD73252.1.
RefSeq	YP_001935726.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6326870.
GenomeReviews	Gene locus BAbS19_I17700 in contig CP000887_GR.
KEGG	bmc:BAbS19_I17700.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CKRALKQ.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BRUA1

Accession

Primary (citable) accession number: B2S843

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)