Reviewed, UniProtKB/Swiss-Prot B2S630 (QUEF_BRUA1)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	BAbS19_I11220

Organism

Brucella abortus (strain S19) [Complete proteome] [HAMAP]

Taxonomic identifier

430066 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm By similarity HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_1000134297

Sequences

	Sequence		Length	Mass (Da)
	B2S630-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: BEC57D5171BBC051	FASTA	155	17,402
	10 20 30 40 50 60 MSENTIYSGL KQLGSHTDIP LTPEEAVLER VANPQEGTPY CVRFTAPEFS SLCPMTGQPD 70 80 90 100 110 120 FAHLVIDYVP GKWLVESKSL KLFLFSFRNH GAFHEDCTVT IGKRLVDLLE PEWLRIGGYW 130 140 150 YPRGGIPIDV FYQTGAAPLN VWIPEQGVAN YRGRG

References

[1]

"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes." Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W. PLoS ONE 3:E2193-E2193(2008) [PubMed: 18478107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000887 Genomic DNA. Translation: ACD72627.1.
RefSeq	YP_001935101.1.
3D structure databases
SMR	B2S630. Positions 55-146.
ModBase	Search...
Genome annotation databases
GeneID	6328670.
GenomeReviews	Gene locus BAbS19_I11220 in contig CP000887_GR.
KEGG	bmc:BAbS19_I11220.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG294103.
OMA	LYLASFR.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BRUA1

Accession

Primary (citable) accession number: B2S630

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)