B2S524 (FABH_BRUA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	BAbS19_I07470

Organism

Brucella abortus (strain S19) [Complete proteome] [HAMAP]

Taxonomic identifier

430066 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm By similarity HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000187846
Regions
	Region	251 – 255	5	ACP-binding By similarity
Sites
	Active site	113	1	By similarity
	Active site	250	1	By similarity
	Active site	280	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2S524 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: B90A5420ADA296C7	FASTA	323	34,448
	10 20 30 40 50 60 MIRSVVRGIG SALPKRVMKN TDFEGIVETS DEWIVQRTGI RERHIAGEGE TTVSLGAAAA 70 80 90 100 110 120 RAAIENAGLQ PSDIDLVLLA TSTPNNTFPA SAVAIQRELG ITRGFAFDLQ AVCSGFIYAI 130 140 150 160 170 180 TTADLYIRGG MARRVLVIGA ETFSRILDWT DRTTCVLFGD GAGAIVLEAA EGHGLTSDRG 190 200 210 220 230 240 ILAANLRSDG NHKEKLYVDG GPSTTQTVGH LRMEGREVFK HAVGMITDVI EASFEATGLT 250 260 270 280 290 300 AEDIDWFVPH QANKRIIDAS AKKLHIAEEK VVITVDRHGN TSAASVPLAL ATAVADGRIK 310 320 KGDLVLLEAM GGGFTWGAVL VRW

References

[1]

"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes." Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B.W. PLoS ONE 3:E2193-E2193(2008) [PubMed: 18478107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S19.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000887 Genomic DNA. Translation: ACD72271.1.
RefSeq	YP_001934745.1. NC_010742.1.
3D structure databases
ProteinModelPortal	B2S524.
ModBase	Search...
Protein-protein interaction databases
STRING	B2S524.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6327906.
GenomeReviews	Gene locus BAbS19_I07470 in contig CP000887_GR.
KEGG	bmc:BAbS19_I07470.
PATRIC	17818526. VBIBruAbo38055_2036.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	CSSDLGL.
ProtClustDB	PRK09352.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_BRUA1

Accession

Primary (citable) accession number: B2S524

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program