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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Treponema pallidum subsp. pallidum (strain SS14)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi125ZincUniRule annotation1
Metal bindingi127ZincUniRule annotation1
Metal bindingi152ZincUniRule annotation1
Metal bindingi154ZincUniRule annotation1
Binding sitei273ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:TPASS_0673
OrganismiTreponema pallidum subsp. pallidum (strain SS14)
Taxonomic identifieri455434 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
Proteomesi
  • UP000001202 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000901171 – 537Glutamate--tRNA ligaseAdd BLAST537

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2S3R2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionAdd BLAST11
Motifi270 – 274"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK01885.
OMAiHGATNVM.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 2 hits.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B2S3R2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVRVRYAPS PTGLQHIGGI RTALFNFLFA RAHAGVFVLR VEDTDRSRCT
60 70 80 90 100
AAFEQNLYDT LRWLGVSWDE GGGCPETAVK QGARGDGRSV AHAGGAYGPY
110 120 130 140 150
TQSARTDLYR AQVARLVETG QAYYCFCDAS RLERVRKIRT LNRMPPGYDR
160 170 180 190 200
HCRELLPEEV RECLASGVPH VIRFKVPLEG STHFRDALLG DIEWQNEEIN
210 220 230 240 250
PDPILLKSDG FPTYHLANVV DDHAMRITHV LRAQEWVPST PLHLLLYRAF
260 270 280 290 300
GWQPPLFCHL PMVMGADGHK LSKRHGATSC DEFRNAGYLP EALLNYVAML
310 320 330 340 350
GCSYGEGQDL FTREQLCAHF SLSRLNKSPA VFDYKKLAWF NGQYIRAKSD
360 370 380 390 400
EQLCALVWPF IANAGVCGHI PADVEAGAVR TRRFADEAPC APTEAQRSML
410 420 430 440 450
MRVIPLIKER LRFLTDAPEL VRCFFQEPSL PEQGVFVPKR LDVAQVRAVL
460 470 480 490 500
VRARGLVHEI VSASEPDVEV LLRAEAEKFG IKLGDFLMPI RVALTGATVS
510 520 530
APLVGTIRIL GASRSCARIE HVIRERFSDD SQGVGGG
Length:537
Mass (Da):59,840
Last modified:July 1, 2008 - v1
Checksum:iBD9D58C627BAE37F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000805 Genomic DNA. Translation: ACD71091.1.
RefSeqiWP_010882118.1. NC_021508.1.

Genome annotation databases

EnsemblBacteriaiACD71091; ACD71091; TPASS_0673.
GeneIDi2611576.
KEGGitpp:TPASS_0673.
PATRICi20529119. VBITrePal10923_0713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000805 Genomic DNA. Translation: ACD71091.1.
RefSeqiWP_010882118.1. NC_021508.1.

3D structure databases

ProteinModelPortaliB2S3R2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD71091; ACD71091; TPASS_0673.
GeneIDi2611576.
KEGGitpp:TPASS_0673.
PATRICi20529119. VBITrePal10923_0713.

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK01885.
OMAiHGATNVM.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 2 hits.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_TREPS
AccessioniPrimary (citable) accession number: B2S3R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: November 30, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.