Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B2S360 (TRMB_TREPS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:TPASS_0464
OrganismTreponema pallidum subsp. pallidum (strain SS14) [Complete proteome] [HAMAP]
Taxonomic identifier455434 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136373

Regions

Region229 – 2324Substrate binding By similarity

Sites

Active site1611 By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1111S-adenosyl-L-methionine By similarity
Binding site1381S-adenosyl-L-methionine By similarity
Binding site1611S-adenosyl-L-methionine By similarity
Binding site1651Substrate By similarity
Binding site1971Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B2S360 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: C5A227F4E4D15CC0

FASTA25028,068
        10         20         30         40         50         60 
MTNDSARMRK VLTFTRRSNR MTACQKRDYQ HLASRWIIPY QNTVFDYAAV FCSPAAPSAP 

        70         80         90        100        110        120 
AGAFPAPQGK TDAVAPACAP APLVVEIGFG MGSATAAIAA RNPHLSYLGI EVYRAGIGRL 

       130        140        150        160        170        180 
LRKIEAERLH NLRIIEHDAL DVLRTMIAPQ TLAGLHIFFP DPWPKTRHHK RRLLYRPRTD 

       190        200        210        220        230        240 
LLARALAPGG YLYAVTDWAE YARRAQEELA RTPSLTWAPQ GARPWRPATE FERKAQTQGR 

       250 
AIHELFFIKA 

« Hide

References

[1]"Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays."
Matejkova P., Strouhal M., Smajs D., Norris S.J., Palzkill T., Petrosino J.F., Sodergren E., Norton J.E., Singh J., Richmond T.A., Molla M.N., Albert T.J., Weinstock G.M.
BMC Microbiol. 8:76-76(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SS14.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000805 Genomic DNA. Translation: ACD70889.1.
RefSeqYP_001933468.1. NC_010741.1.
YP_008119210.1. NC_021508.1.

3D structure databases

ProteinModelPortalB2S360.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING455434.TPASS_0464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD70889; ACD70889; TPASS_0464.
GeneID15843324.
6333686.
KEGGtpp:TPASS_0464.
PATRIC20528659. VBITrePal10923_0500.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMATEILQDC.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK2300221.

Enzyme and pathway databases

BioCycTPAL455434:GJ9E-488-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_TREPS
AccessionPrimary (citable) accession number: B2S360
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways