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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Treponema pallidum subsp. pallidum (strain SS14)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861S-adenosyl-L-methionineUniRule annotation
Binding sitei111 – 1111S-adenosyl-L-methionineUniRule annotation
Binding sitei138 – 1381S-adenosyl-L-methionineUniRule annotation
Active sitei161 – 1611By similarity
Binding sitei161 – 1611S-adenosyl-L-methionineUniRule annotation
Binding sitei165 – 1651SubstrateUniRule annotation
Binding sitei197 – 1971SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTPAL455434:GJ9E-488-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:TPASS_0464
OrganismiTreponema pallidum subsp. pallidum (strain SS14)
Taxonomic identifieri455434 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000001202 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250tRNA (guanine-N(7)-)-methyltransferasePRO_1000136373Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi455434.TPASS_0464.

Structurei

3D structure databases

ProteinModelPortaliB2S360.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2324Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiHYKRRFV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2S360-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNDSARMRK VLTFTRRSNR MTACQKRDYQ HLASRWIIPY QNTVFDYAAV
60 70 80 90 100
FCSPAAPSAP AGAFPAPQGK TDAVAPACAP APLVVEIGFG MGSATAAIAA
110 120 130 140 150
RNPHLSYLGI EVYRAGIGRL LRKIEAERLH NLRIIEHDAL DVLRTMIAPQ
160 170 180 190 200
TLAGLHIFFP DPWPKTRHHK RRLLYRPRTD LLARALAPGG YLYAVTDWAE
210 220 230 240 250
YARRAQEELA RTPSLTWAPQ GARPWRPATE FERKAQTQGR AIHELFFIKA
Length:250
Mass (Da):28,068
Last modified:July 1, 2008 - v1
Checksum:iC5A227F4E4D15CC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000805 Genomic DNA. Translation: ACD70889.1.
RefSeqiWP_010881913.1. NC_021508.1.
YP_001933468.1. NC_010741.1.
YP_008119210.1. NC_021508.1.

Genome annotation databases

EnsemblBacteriaiACD70889; ACD70889; TPASS_0464.
GeneIDi2611075.
KEGGitpp:TPASS_0464.
PATRICi20528659. VBITrePal10923_0500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000805 Genomic DNA. Translation: ACD70889.1.
RefSeqiWP_010881913.1. NC_021508.1.
YP_001933468.1. NC_010741.1.
YP_008119210.1. NC_021508.1.

3D structure databases

ProteinModelPortaliB2S360.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455434.TPASS_0464.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD70889; ACD70889; TPASS_0464.
GeneIDi2611075.
KEGGitpp:TPASS_0464.
PATRICi20528659. VBITrePal10923_0500.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiHYKRRFV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciTPAL455434:GJ9E-488-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays."
    Matejkova P., Strouhal M., Smajs D., Norris S.J., Palzkill T., Petrosino J.F., Sodergren E., Norton J.E., Singh J., Richmond T.A., Molla M.N., Albert T.J., Weinstock G.M.
    BMC Microbiol. 8:76-76(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SS14.

Entry informationi

Entry nameiTRMB_TREPS
AccessioniPrimary (citable) accession number: B2S360
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: May 27, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.