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B2S348

- SYI_TREPS

UniProt

B2S348 - SYI_TREPS

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Protein
Isoleucine--tRNA ligase
Gene
ileS, TPASS_0452
Organism
Treponema pallidum subsp. pallidum (strain SS14)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei628 – 6281ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTPAL455434:GJ9E-476-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:TPASS_0452
OrganismiTreponema pallidum subsp. pallidum (strain SS14)
Taxonomic identifieri455434 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000001202: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10911091Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216260Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi455434.TPASS_0452.

Structurei

3D structure databases

ProteinModelPortaliB2S348.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi625 – 6295"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B2S348-1 [UniParc]FASTAAdd to Basket

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MYTPVDPKVD FVAQERRILA FWRERRVFEQ SVAQRAQGKS YVFFDGPPFA     50
TGLPHFGHFV PSTIKDIIPR YQTMRGAYVP RRFGWDCHGL PIEHLIEQEL 100
NLNSKSDVES YGVSAFNAAC RSSVLRYVKE WQRTLTRLGR WVDFDNDYKT 150
MDVCYMESVW WVVAQLWQRK LLYEGYKILP YCPRCATALS NHELNLGGYQ 200
DVSDPAITVR FECTSVVPGS PAAREFCAAA SWGSASLPAH TCFLAWTTTP 250
WTLPCNAALA LGPQILYVLI EANDEHYILA RSRLEFYYPD SSAYRVVWEK 300
RGEHLAGIRY RPLFSYPVFG QGPDPSVQGD SEEGLFCTRV ADFVSTEDGT 350
GVVHVAPAFG EDDYEVFKDA GISIQCPLDA ECRFTAEVAD YQGLFVKAAD 400
KAIIARVQKQ GALFRREQIS HAYPHCWRCA SPLIYRAVHS WFVAVEKIKD 450
KMLAANASIC WQPSHIRDGR FGKWLVCARD WAISRDRYWG NPLPIWRCVH 500
CGATDCIGSR TQLYERSGML LEDLHKHVVD MVTIPCACGS VMRRVPEVLD 550
CWFESGAMPY AQQHYPFEHA TDFERYFPAH FISEGLDQTR GWFYTLTILA 600
VALFERPAFE NCIVTGLVLA SDGKKMSKAL RNYADPNEVM DRYGADALRL 650
FLVRSAVVRA DDLKYSDEGV KDILKTVIIP LWNSYSFYVT YANIDGIDPP 700
VCAKVDGMGQ AVTRLATHLN NPLDRWILSL TEKLVQDIAC ALDAYDVSKV 750
ADPIVSYVDQ LNNWYIRRSR RRFWKSINDE DKRCAYNTLY CVLKRCVLAI 800
APVVPFITES IWQNIRAADD VQSVHLADYP VCTPMVRDDA LEFKMETVQR 850
VVSMARAIRA QCNLKVRQPL KAMQVITRNP MERSALLEME EDVLDELNVK 900
ELVFHEKEDE IVEYRAKANF RVLGKELGSK TKRAALSIER LSSAEIQEIL 950
EGTTLYLDVD GDRLELTEEK ILVQRIERES LKAINEGTLT VALDTTLTED 1000
LLLEGAIRDL VRGVQNLRKE RGFSLVDRIC LRVFSSDQDI VCARKAYDLH 1050
RSYIVGETLA AHVQWARVRD GASAVYVKSD AVLWEVSIDK A 1091
Length:1,091
Mass (Da):124,242
Last modified:July 1, 2008 - v1
Checksum:i30FCAE18C6F301A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000805 Genomic DNA. Translation: ACD70877.1.
RefSeqiYP_001933456.1. NC_010741.1.
YP_008119199.1. NC_021508.1.

Genome annotation databases

EnsemblBacteriaiACD70877; ACD70877; TPASS_0452.
GeneIDi15843313.
6333693.
KEGGitpp:TPASS_0452.
PATRICi20528627. VBITrePal10923_0484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000805 Genomic DNA. Translation: ACD70877.1 .
RefSeqi YP_001933456.1. NC_010741.1.
YP_008119199.1. NC_021508.1.

3D structure databases

ProteinModelPortali B2S348.
ModBasei Search...

Protein-protein interaction databases

STRINGi 455434.TPASS_0452.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD70877 ; ACD70877 ; TPASS_0452 .
GeneIDi 15843313.
6333693.
KEGGi tpp:TPASS_0452.
PATRICi 20528627. VBITrePal10923_0484.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TPAL455434:GJ9E-476-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays."
    Matejkova P., Strouhal M., Smajs D., Norris S.J., Palzkill T., Petrosino J.F., Sodergren E., Norton J.E., Singh J., Richmond T.A., Molla M.N., Albert T.J., Weinstock G.M.
    BMC Microbiol. 8:76-76(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SS14.

Entry informationi

Entry nameiSYI_TREPS
AccessioniPrimary (citable) accession number: B2S348
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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