B2S348 (SYI_TREPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	TPASS_0452

Organism

Treponema pallidum subsp. pallidum (strain SS14) [Complete proteome] [HAMAP]

Taxonomic identifier

455434 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Spirochaetaceae › Treponema › Treponema pallidum › Treponema pallidum subsp. pallidum

Protein attributes

Sequence length	1091 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003
Cofactor	Zinc By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1091	1091	Isoleucine--tRNA ligase HAMAP-Rule MF_02003		PRO_1000216260
Regions
	Motif	48 – 58	11	"HIGH" region HAMAP-Rule MF_02003
	Motif	625 – 629	5	"KMSKS" region HAMAP-Rule MF_02003
Sites
	Binding site	628	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2S348 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 30FCAE18C6F301A2	FASTA	1,091	124,242
	10 20 30 40 50 60 MYTPVDPKVD FVAQERRILA FWRERRVFEQ SVAQRAQGKS YVFFDGPPFA TGLPHFGHFV 70 80 90 100 110 120 PSTIKDIIPR YQTMRGAYVP RRFGWDCHGL PIEHLIEQEL NLNSKSDVES YGVSAFNAAC 130 140 150 160 170 180 RSSVLRYVKE WQRTLTRLGR WVDFDNDYKT MDVCYMESVW WVVAQLWQRK LLYEGYKILP 190 200 210 220 230 240 YCPRCATALS NHELNLGGYQ DVSDPAITVR FECTSVVPGS PAAREFCAAA SWGSASLPAH 250 260 270 280 290 300 TCFLAWTTTP WTLPCNAALA LGPQILYVLI EANDEHYILA RSRLEFYYPD SSAYRVVWEK 310 320 330 340 350 360 RGEHLAGIRY RPLFSYPVFG QGPDPSVQGD SEEGLFCTRV ADFVSTEDGT GVVHVAPAFG 370 380 390 400 410 420 EDDYEVFKDA GISIQCPLDA ECRFTAEVAD YQGLFVKAAD KAIIARVQKQ GALFRREQIS 430 440 450 460 470 480 HAYPHCWRCA SPLIYRAVHS WFVAVEKIKD KMLAANASIC WQPSHIRDGR FGKWLVCARD 490 500 510 520 530 540 WAISRDRYWG NPLPIWRCVH CGATDCIGSR TQLYERSGML LEDLHKHVVD MVTIPCACGS 550 560 570 580 590 600 VMRRVPEVLD CWFESGAMPY AQQHYPFEHA TDFERYFPAH FISEGLDQTR GWFYTLTILA 610 620 630 640 650 660 VALFERPAFE NCIVTGLVLA SDGKKMSKAL RNYADPNEVM DRYGADALRL FLVRSAVVRA 670 680 690 700 710 720 DDLKYSDEGV KDILKTVIIP LWNSYSFYVT YANIDGIDPP VCAKVDGMGQ AVTRLATHLN 730 740 750 760 770 780 NPLDRWILSL TEKLVQDIAC ALDAYDVSKV ADPIVSYVDQ LNNWYIRRSR RRFWKSINDE 790 800 810 820 830 840 DKRCAYNTLY CVLKRCVLAI APVVPFITES IWQNIRAADD VQSVHLADYP VCTPMVRDDA 850 860 870 880 890 900 LEFKMETVQR VVSMARAIRA QCNLKVRQPL KAMQVITRNP MERSALLEME EDVLDELNVK 910 920 930 940 950 960 ELVFHEKEDE IVEYRAKANF RVLGKELGSK TKRAALSIER LSSAEIQEIL EGTTLYLDVD 970 980 990 1000 1010 1020 GDRLELTEEK ILVQRIERES LKAINEGTLT VALDTTLTED LLLEGAIRDL VRGVQNLRKE 1030 1040 1050 1060 1070 1080 RGFSLVDRIC LRVFSSDQDI VCARKAYDLH RSYIVGETLA AHVQWARVRD GASAVYVKSD 1090 AVLWEVSIDK A

References

[1]

"Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays." Matejkova P., Strouhal M., Smajs D., Norris S.J., Palzkill T., Petrosino J.F., Sodergren E., Norton J.E., Singh J., Richmond T.A., Molla M.N., Albert T.J., Weinstock G.M. BMC Microbiol. 8:76-76(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SS14.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000805 Genomic DNA. Translation: ACD70877.1.
RefSeq	YP_001933456.1. NC_010741.1.
3D structure databases
ProteinModelPortal	B2S348.
ModBase	Search...
Protein-protein interaction databases
STRING	455434.TPASS_0452.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD70877; ACD70877; TPASS_0452.
GeneID	6333693.
KEGG	tpp:TPASS_0452.
PATRIC	20528627. VBITrePal10923_0484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246403.
KO	K01870.
OMA	SRSRYWG.
ProtClustDB	PRK06039.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02003. Ile_tRNA_synth_type2.
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_TREPS

Accession

Primary (citable) accession number: B2S348

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program