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B2S348

- SYI_TREPS

UniProt

B2S348 - SYI_TREPS

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Treponema pallidum subsp. pallidum (strain SS14)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei628 – 6281ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTPAL455434:GJ9E-476-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:TPASS_0452
    OrganismiTreponema pallidum subsp. pallidum (strain SS14)
    Taxonomic identifieri455434 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
    ProteomesiUP000001202: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10911091Isoleucine--tRNA ligasePRO_1000216260Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi455434.TPASS_0452.

    Structurei

    3D structure databases

    ProteinModelPortaliB2S348.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi625 – 6295"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2S348-1 [UniParc]FASTAAdd to Basket

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    MYTPVDPKVD FVAQERRILA FWRERRVFEQ SVAQRAQGKS YVFFDGPPFA     50
    TGLPHFGHFV PSTIKDIIPR YQTMRGAYVP RRFGWDCHGL PIEHLIEQEL 100
    NLNSKSDVES YGVSAFNAAC RSSVLRYVKE WQRTLTRLGR WVDFDNDYKT 150
    MDVCYMESVW WVVAQLWQRK LLYEGYKILP YCPRCATALS NHELNLGGYQ 200
    DVSDPAITVR FECTSVVPGS PAAREFCAAA SWGSASLPAH TCFLAWTTTP 250
    WTLPCNAALA LGPQILYVLI EANDEHYILA RSRLEFYYPD SSAYRVVWEK 300
    RGEHLAGIRY RPLFSYPVFG QGPDPSVQGD SEEGLFCTRV ADFVSTEDGT 350
    GVVHVAPAFG EDDYEVFKDA GISIQCPLDA ECRFTAEVAD YQGLFVKAAD 400
    KAIIARVQKQ GALFRREQIS HAYPHCWRCA SPLIYRAVHS WFVAVEKIKD 450
    KMLAANASIC WQPSHIRDGR FGKWLVCARD WAISRDRYWG NPLPIWRCVH 500
    CGATDCIGSR TQLYERSGML LEDLHKHVVD MVTIPCACGS VMRRVPEVLD 550
    CWFESGAMPY AQQHYPFEHA TDFERYFPAH FISEGLDQTR GWFYTLTILA 600
    VALFERPAFE NCIVTGLVLA SDGKKMSKAL RNYADPNEVM DRYGADALRL 650
    FLVRSAVVRA DDLKYSDEGV KDILKTVIIP LWNSYSFYVT YANIDGIDPP 700
    VCAKVDGMGQ AVTRLATHLN NPLDRWILSL TEKLVQDIAC ALDAYDVSKV 750
    ADPIVSYVDQ LNNWYIRRSR RRFWKSINDE DKRCAYNTLY CVLKRCVLAI 800
    APVVPFITES IWQNIRAADD VQSVHLADYP VCTPMVRDDA LEFKMETVQR 850
    VVSMARAIRA QCNLKVRQPL KAMQVITRNP MERSALLEME EDVLDELNVK 900
    ELVFHEKEDE IVEYRAKANF RVLGKELGSK TKRAALSIER LSSAEIQEIL 950
    EGTTLYLDVD GDRLELTEEK ILVQRIERES LKAINEGTLT VALDTTLTED 1000
    LLLEGAIRDL VRGVQNLRKE RGFSLVDRIC LRVFSSDQDI VCARKAYDLH 1050
    RSYIVGETLA AHVQWARVRD GASAVYVKSD AVLWEVSIDK A 1091
    Length:1,091
    Mass (Da):124,242
    Last modified:July 1, 2008 - v1
    Checksum:i30FCAE18C6F301A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000805 Genomic DNA. Translation: ACD70877.1.
    RefSeqiYP_001933456.1. NC_010741.1.
    YP_008119199.1. NC_021508.1.

    Genome annotation databases

    EnsemblBacteriaiACD70877; ACD70877; TPASS_0452.
    GeneIDi15843313.
    6333693.
    KEGGitpp:TPASS_0452.
    PATRICi20528627. VBITrePal10923_0484.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000805 Genomic DNA. Translation: ACD70877.1 .
    RefSeqi YP_001933456.1. NC_010741.1.
    YP_008119199.1. NC_021508.1.

    3D structure databases

    ProteinModelPortali B2S348.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 455434.TPASS_0452.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD70877 ; ACD70877 ; TPASS_0452 .
    GeneIDi 15843313.
    6333693.
    KEGGi tpp:TPASS_0452.
    PATRICi 20528627. VBITrePal10923_0484.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TPAL455434:GJ9E-476-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays."
      Matejkova P., Strouhal M., Smajs D., Norris S.J., Palzkill T., Petrosino J.F., Sodergren E., Norton J.E., Singh J., Richmond T.A., Molla M.N., Albert T.J., Weinstock G.M.
      BMC Microbiol. 8:76-76(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SS14.

    Entry informationi

    Entry nameiSYI_TREPS
    AccessioniPrimary (citable) accession number: B2S348
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3