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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Treponema pallidum subsp. pallidum (strain SS14)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711SubstrateUniRule annotation
Binding sitei215 – 2151Substrate; via amide nitrogenUniRule annotation
Metal bindingi222 – 2221ManganeseUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Metal bindingi242 – 2421Manganese; via tele nitrogenUniRule annotation
Binding sitei264 – 2641SubstrateUniRule annotation
Active sitei266 – 2661UniRule annotation
Metal bindingi289 – 2891ManganeseUniRule annotation
Binding sitei387 – 3871GTPUniRule annotation
Binding sitei418 – 4181GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi265 – 2706GTPUniRule annotation
Nucleotide bindingi514 – 5174GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTPAL455434:GJ9E-129-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:TPASS_0122
OrganismiTreponema pallidum subsp. pallidum (strain SS14)
Taxonomic identifieri455434 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000001202 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Phosphoenolpyruvate carboxykinase [GTP]PRO_1000125048Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi455434.TPASS_0122.

Structurei

3D structure databases

ProteinModelPortaliB2S270.
SMRiB2S270. Positions 9-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni385 – 3873Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2S270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELHEIAHAR AKAWIEEMVA LCAPDTVYVC DGSKKEYDTI MQKMVDAGLA
60 70 80 90 100
TPLKKRKNCF LFRSQPSDVA RVEARTFIAS KREDDAGPTN HWTDPAELKK
110 120 130 140 150
TMTGLYSQCM KGRTMYVIPF SMGPVGSPIS KNGIEITDSE YVVCNMHIMT
160 170 180 190 200
RVGTRVLEAL GTDGEFVPCL HSVGKPLGPG VTDAGQWPCA DMERKYISHF
210 220 230 240 250
PEERLVWSFG SGYGGNALLG KKCFALRIAS VLARDEGWLA EHMLILKITN
260 270 280 290 300
PAGKTKYIGA AFPSACGKTN LAMMIPTLPG WKVETVGDDI AWMKFGKDGR
310 320 330 340 350
LYAINPEAGF FGVAPGTSDF SNKNAMDSIK ENAIFTNCGL TEDGDVWWEG
360 370 380 390 400
IGYPAKGTII DWHGVSRPAP TRDKSPKGEE IAHPNARFTA PARQCPAIAS
410 420 430 440 450
NWEDPEGVPI DAFLFGGRRP STVPLVHQAR DWNHGVFLGS IIGSEVTAAV
460 470 480 490 500
ISDQVGQIRR DPFAMLPFCG YHMADYFSHW IKLGSQARAE NLPKIFCVNW
510 520 530 540 550
FRKDAEGNFL WPGYGDNSRV LAWIFDRCDG VDNAVETAIG WMPKEGALNT
560 570 580 590 600
EGLNVSTQAV KELLSVDIAG WKKEIKDIRE NHYPKFGARL PQQLRDALEV
610
LEARINGSEG AACTRDMC
Length:618
Mass (Da):68,125
Last modified:June 30, 2008 - v1
Checksum:i4230B8F77DDD4AA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000805 Genomic DNA. Translation: ACD70549.1.
RefSeqiYP_001933128.1. NC_010741.1.
YP_008118895.1. NC_021508.1.

Genome annotation databases

EnsemblBacteriaiACD70549; ACD70549; TPASS_0122.
KEGGitpp:TPASS_0122.
PATRICi20527891. VBITrePal10923_0130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000805 Genomic DNA. Translation: ACD70549.1.
RefSeqiYP_001933128.1. NC_010741.1.
YP_008118895.1. NC_021508.1.

3D structure databases

ProteinModelPortaliB2S270.
SMRiB2S270. Positions 9-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455434.TPASS_0122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD70549; ACD70549; TPASS_0122.
KEGGitpp:TPASS_0122.
PATRICi20527891. VBITrePal10923_0130.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciTPAL455434:GJ9E-129-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays."
    Matejkova P., Strouhal M., Smajs D., Norris S.J., Palzkill T., Petrosino J.F., Sodergren E., Norton J.E., Singh J., Richmond T.A., Molla M.N., Albert T.J., Weinstock G.M.
    BMC Microbiol. 8:76-76(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SS14.

Entry informationi

Entry nameiPCKG_TREPS
AccessioniPrimary (citable) accession number: B2S270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2009
Last sequence update: June 30, 2008
Last modified: March 31, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.