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B2S1H8

- SYI_BORHD

UniProt

B2S1H8 - SYI_BORHD

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia hermsii (strain HS1 / DAH)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBHER314723:GJES-843-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BH0833
OrganismiBorrelia hermsii (strain HS1 / DAH)
Taxonomic identifieri314723 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000008834: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Isoleucine--tRNA ligasePRO_1000216250Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi314723.BH0833.

Structurei

3D structure databases

ProteinModelPortaliB2S1H8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B2S1H8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKKVESKVN FPKIEEKVLK FWNDNKIFEK SMQQREGCEE FTFYDGPPFA
60 70 80 90 100
TGLPHFGHFV PNTIKDIIPR YKTMKGKHVK RYFGWDTHGL PVEYEVEKSL
110 120 130 140 150
QISGRYEIEK YGVDKFNEEC KNIVLRYTKE WQKTISRLGR WVDFEHNYKT
160 170 180 190 200
MDTTFMESVW WVFQTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK
210 220 230 240 250
EVHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGR DIDYSKIFDK
260 270 280 290 300
EKNETFIIGT KRLNHYYKDD KTYTVIEQFK GEHIKGIEYE PVFDYFLSQR
310 320 330 340 350
NKGAFRIHTA EYVTTDDGTG IVHIAPFGEE DYNILKKNTK TDMITPIDAE
360 370 380 390 400
CRFTNEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENFLH RYPFCYRTNS
410 420 430 440 450
PLIYRPISSW FVNIEAIKEK LIKSNEQINW IPSHLKKGRF GKWLENARDW
460 470 480 490 500
AISRNRFWGN PIPVWICSKT GNKICIGSKE ELERLSGQKV NDLHKDKIDK
510 520 530 540 550
ITWPSQYGGT YVRTSEVLDC WFESGSMPYA SKHYPFKDKD KFHDIFPADF
560 570 580 590 600
IAEGLDQTRG WFYTLTILGT ALFEQTAFKN VIVNGLVLSS DGRKMSKSLR
610 620 630 640 650
NYTDPMEVIN TFGADALRLY LVMSPVVRAD DLKYSDDGVK DVLKNIIIPI
660 670 680 690 700
WNAYSFFITY AIIDKFEPNS NIVLYKTNIL DKWIISEIES LKKTLNEEID
710 720 730 740 750
KYNLTKSIEE LLAFIDKLNN WYIRRSRRRF WKSENDNDKI DAYETLYYAL
760 770 780 790 800
KNLMLMLAPF IPFLTEEIYQ NLKTKDEKES IHLNEYPQEI KELINIDLEE
810 820 830 840 850
KMNFIRKVVS IARALRASHN IKIRKPISTI YIVTKDQKEQ QILSEMKEII
860 870 880 890 900
LEEINSEEIK IKSNEEELVT YKAKANFREL GSKLGTNMKA VALEIMKLNN
910 920 930 940 950
EDILKIINGN KHTIKIKDNT YDITLKDIIL ERHEKENLKV INEDSVTIGL
960 970 980 990 1000
DALITEELYL KGLSRELIRK VQNLRKENNF NVSDRIILYI DNNEILKKII
1010 1020 1030 1040
SQFESYIKNE TLTLKIEINN EKALTKVELD DEILIKIGIE KWSN
Length:1,044
Mass (Da):122,955
Last modified:July 1, 2008 - v1
Checksum:iEEF5E826DF30426D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000048 Genomic DNA. Translation: AAX17330.1.
RefSeqiYP_001884254.1. NC_010673.1.

Genome annotation databases

EnsemblBacteriaiAAX17330; AAX17330; BH0833.
GeneIDi6277128.
KEGGibhr:BH0833.
PATRICi20569062. VBIBorHer61105_0842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000048 Genomic DNA. Translation: AAX17330.1 .
RefSeqi YP_001884254.1. NC_010673.1.

3D structure databases

ProteinModelPortali B2S1H8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314723.BH0833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX17330 ; AAX17330 ; BH0833 .
GeneIDi 6277128.
KEGGi bhr:BH0833.
PATRICi 20569062. VBIBorHer61105_0842.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BHER314723:GJES-843-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
    Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HS1 / DAH.

Entry informationi

Entry nameiSYI_BORHD
AccessioniPrimary (citable) accession number: B2S1H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3