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B2S1H8

- SYI_BORHD

UniProt

B2S1H8 - SYI_BORHD

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia hermsii (strain HS1 / DAH)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei597 – 5971ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBHER314723:GJES-843-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BH0833
    OrganismiBorrelia hermsii (strain HS1 / DAH)
    Taxonomic identifieri314723 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
    ProteomesiUP000008834: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10441044Isoleucine--tRNA ligasePRO_1000216250Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi314723.BH0833.

    Structurei

    3D structure databases

    ProteinModelPortaliB2S1H8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKWIISEI.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2S1H8-1 [UniParc]FASTAAdd to Basket

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    MFKKVESKVN FPKIEEKVLK FWNDNKIFEK SMQQREGCEE FTFYDGPPFA     50
    TGLPHFGHFV PNTIKDIIPR YKTMKGKHVK RYFGWDTHGL PVEYEVEKSL 100
    QISGRYEIEK YGVDKFNEEC KNIVLRYTKE WQKTISRLGR WVDFEHNYKT 150
    MDTTFMESVW WVFQTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
    EVHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGR DIDYSKIFDK 250
    EKNETFIIGT KRLNHYYKDD KTYTVIEQFK GEHIKGIEYE PVFDYFLSQR 300
    NKGAFRIHTA EYVTTDDGTG IVHIAPFGEE DYNILKKNTK TDMITPIDAE 350
    CRFTNEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENFLH RYPFCYRTNS 400
    PLIYRPISSW FVNIEAIKEK LIKSNEQINW IPSHLKKGRF GKWLENARDW 450
    AISRNRFWGN PIPVWICSKT GNKICIGSKE ELERLSGQKV NDLHKDKIDK 500
    ITWPSQYGGT YVRTSEVLDC WFESGSMPYA SKHYPFKDKD KFHDIFPADF 550
    IAEGLDQTRG WFYTLTILGT ALFEQTAFKN VIVNGLVLSS DGRKMSKSLR 600
    NYTDPMEVIN TFGADALRLY LVMSPVVRAD DLKYSDDGVK DVLKNIIIPI 650
    WNAYSFFITY AIIDKFEPNS NIVLYKTNIL DKWIISEIES LKKTLNEEID 700
    KYNLTKSIEE LLAFIDKLNN WYIRRSRRRF WKSENDNDKI DAYETLYYAL 750
    KNLMLMLAPF IPFLTEEIYQ NLKTKDEKES IHLNEYPQEI KELINIDLEE 800
    KMNFIRKVVS IARALRASHN IKIRKPISTI YIVTKDQKEQ QILSEMKEII 850
    LEEINSEEIK IKSNEEELVT YKAKANFREL GSKLGTNMKA VALEIMKLNN 900
    EDILKIINGN KHTIKIKDNT YDITLKDIIL ERHEKENLKV INEDSVTIGL 950
    DALITEELYL KGLSRELIRK VQNLRKENNF NVSDRIILYI DNNEILKKII 1000
    SQFESYIKNE TLTLKIEINN EKALTKVELD DEILIKIGIE KWSN 1044
    Length:1,044
    Mass (Da):122,955
    Last modified:July 1, 2008 - v1
    Checksum:iEEF5E826DF30426D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000048 Genomic DNA. Translation: AAX17330.1.
    RefSeqiYP_001884254.1. NC_010673.1.

    Genome annotation databases

    EnsemblBacteriaiAAX17330; AAX17330; BH0833.
    GeneIDi6277128.
    KEGGibhr:BH0833.
    PATRICi20569062. VBIBorHer61105_0842.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000048 Genomic DNA. Translation: AAX17330.1 .
    RefSeqi YP_001884254.1. NC_010673.1.

    3D structure databases

    ProteinModelPortali B2S1H8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 314723.BH0833.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX17330 ; AAX17330 ; BH0833 .
    GeneIDi 6277128.
    KEGGi bhr:BH0833.
    PATRICi 20569062. VBIBorHer61105_0842.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KWIISEI.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BHER314723:GJES-843-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
      Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
      Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HS1 / DAH.

    Entry informationi

    Entry nameiSYI_BORHD
    AccessioniPrimary (citable) accession number: B2S1H8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3