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B2S1H8

- SYI_BORHD

UniProt

B2S1H8 - SYI_BORHD

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BH0833
Organism
Borrelia hermsii (strain HS1 / DAH)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBHER314723:GJES-843-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BH0833
OrganismiBorrelia hermsii (strain HS1 / DAH)
Taxonomic identifieri314723 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000008834: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216250Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi314723.BH0833.

Structurei

3D structure databases

ProteinModelPortaliB2S1H8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B2S1H8-1 [UniParc]FASTAAdd to Basket

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MFKKVESKVN FPKIEEKVLK FWNDNKIFEK SMQQREGCEE FTFYDGPPFA     50
TGLPHFGHFV PNTIKDIIPR YKTMKGKHVK RYFGWDTHGL PVEYEVEKSL 100
QISGRYEIEK YGVDKFNEEC KNIVLRYTKE WQKTISRLGR WVDFEHNYKT 150
MDTTFMESVW WVFQTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
EVHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGR DIDYSKIFDK 250
EKNETFIIGT KRLNHYYKDD KTYTVIEQFK GEHIKGIEYE PVFDYFLSQR 300
NKGAFRIHTA EYVTTDDGTG IVHIAPFGEE DYNILKKNTK TDMITPIDAE 350
CRFTNEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENFLH RYPFCYRTNS 400
PLIYRPISSW FVNIEAIKEK LIKSNEQINW IPSHLKKGRF GKWLENARDW 450
AISRNRFWGN PIPVWICSKT GNKICIGSKE ELERLSGQKV NDLHKDKIDK 500
ITWPSQYGGT YVRTSEVLDC WFESGSMPYA SKHYPFKDKD KFHDIFPADF 550
IAEGLDQTRG WFYTLTILGT ALFEQTAFKN VIVNGLVLSS DGRKMSKSLR 600
NYTDPMEVIN TFGADALRLY LVMSPVVRAD DLKYSDDGVK DVLKNIIIPI 650
WNAYSFFITY AIIDKFEPNS NIVLYKTNIL DKWIISEIES LKKTLNEEID 700
KYNLTKSIEE LLAFIDKLNN WYIRRSRRRF WKSENDNDKI DAYETLYYAL 750
KNLMLMLAPF IPFLTEEIYQ NLKTKDEKES IHLNEYPQEI KELINIDLEE 800
KMNFIRKVVS IARALRASHN IKIRKPISTI YIVTKDQKEQ QILSEMKEII 850
LEEINSEEIK IKSNEEELVT YKAKANFREL GSKLGTNMKA VALEIMKLNN 900
EDILKIINGN KHTIKIKDNT YDITLKDIIL ERHEKENLKV INEDSVTIGL 950
DALITEELYL KGLSRELIRK VQNLRKENNF NVSDRIILYI DNNEILKKII 1000
SQFESYIKNE TLTLKIEINN EKALTKVELD DEILIKIGIE KWSN 1044
Length:1,044
Mass (Da):122,955
Last modified:July 1, 2008 - v1
Checksum:iEEF5E826DF30426D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000048 Genomic DNA. Translation: AAX17330.1.
RefSeqiYP_001884254.1. NC_010673.1.

Genome annotation databases

EnsemblBacteriaiAAX17330; AAX17330; BH0833.
GeneIDi6277128.
KEGGibhr:BH0833.
PATRICi20569062. VBIBorHer61105_0842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000048 Genomic DNA. Translation: AAX17330.1 .
RefSeqi YP_001884254.1. NC_010673.1.

3D structure databases

ProteinModelPortali B2S1H8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 314723.BH0833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX17330 ; AAX17330 ; BH0833 .
GeneIDi 6277128.
KEGGi bhr:BH0833.
PATRICi 20569062. VBIBorHer61105_0842.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BHER314723:GJES-843-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
    Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HS1 / DAH.

Entry informationi

Entry nameiSYI_BORHD
AccessioniPrimary (citable) accession number: B2S1H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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