Reviewed, UniProtKB/Swiss-Prot B2S1B0 (GCP_BORHD)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BH0769

Organism

Borrelia hermsii (strain DAH) [Complete proteome] [HAMAP]

Taxonomic identifier

314723 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc By similarity. HAMAP MF_01445
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_1000145950
Sites
	Metal binding	110	1	Zinc Potential
	Metal binding	114	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	B2S1B0-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 164CF179D149535F	FASTA	338	37,469
	10 20 30 40 50 60 MIVLGIESSC DDCCAAIVED GVKILSNIKL SQKEHKKYYG VVPEIASRLH TEFIMSVCQK 70 80 90 100 110 120 AITNAQIHAS EIDLIAVTSK PGLIGSLIVG INFAKGLSIA LKKPLICIDH ILGHLYAPLM 130 140 150 160 170 180 TIKIEYPFLS LILSGGHTIL AKQNDFDDIE ILGRTLDDAC GEAFDKVAKH YKMGFPGGPN 190 200 210 220 230 240 IEKLAKCGNQ YAFNFPITIF DKKENRYDFS YSGLKTACIH QLEKFKDKNE NITNNNIAAS 250 260 270 280 290 300 FQRVAFENLI IPIKRAIKDT NIKKLIISGG VASNLYLREK IKNLEVETYY PPIDLCTDNG 310 320 330 AMIAGIGYLM YLKYGASPIE TDANSRIENY KYTKGAKL

References

[1]

"The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever." Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000048 Genomic DNA. Translation: AAX17264.1.
RefSeq	YP_001884186.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	6277083.
GenomeReviews	Gene locus BH0769 in contig CP000048_GR.
KEGG	bhr:BH0769.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304663.
OMA	CKRALKQ.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BORHD

Accession

Primary (citable) accession number: B2S1B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)