B2S162 (SYT_BORHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BH0720

Organism

Borrelia hermsii (strain DAH) [Complete proteome] [HAMAP]

Taxonomic identifier

314723 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000098544
Regions
	Region	185 – 478	294	Catalytic HAMAP MF_00184
Sites
	Metal binding	278	1	Zinc; catalytic By similarity
	Metal binding	329	1	Zinc; catalytic By similarity
	Metal binding	455	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2S162 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: F7759BCB7D0C2BF0	FASTA	583	68,115
	10 20 30 40 50 60 MSEKLDRESI LYKKRHSIAH VMAEAVLELF PNTKIAIGPP IKDGFYYDFD FEKHITEDDL 70 80 90 100 110 120 LLIEQKMREI LKTGSPFVKE VITKEQALML FKDEPYKMDL IRGLAITDEI SIYKSHNFTD 130 140 150 160 170 180 LCKGPHVDNM GKIDPKAFKL TSIAGAYWRG DEKNKMLTRI YGTLWNNEKD LKAYLKLREE 190 200 210 220 230 240 IKRRDHRKLG RELDLFSVHE DIGPGLIFFH PNGARIRAII EDFWREEHFK NGYDVLFTPH 250 260 270 280 290 300 IGKSCLWETS GHLDFYKESM FEKMEMDKSN YYVKPMNCPF HIAIYNTGKH SYRDLPFRWA 310 320 330 340 350 360 ELGTVYRYEK IGALHGTMRV RGFTQDDAHI ICAYEQVKSE VQEVLRFALY MWNQFGFADL 370 380 390 400 410 420 KAYLSTKPEK AVGDKDDWEM SVKVLEEALI DLNIAYDIDE GGGAFYGPKI DLKIIDSLGR 430 440 450 460 470 480 EWQMSTIQFD FNLPERFKMT YTAEDGKEKR PFMIHRALLG SIERFFGILV EHYGGAFPVW 490 500 510 520 530 540 LAPLQVIIIP VNNVVEEYAL EILSLFKNEG IRIKLDNDCN MRMNAKIRQY QSKKVPYMFI 550 560 570 580 IGEKEVVEGK ISIRTRTNDQ INGLELKEAL EFVRLKVKNK EIL

References

[1]

"The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever." Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DAH.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000048 Genomic DNA. Translation: AAX17218.1.
RefSeq	YP_001884138.1. NC_010673.1.
3D structure databases
ProteinModelPortal	B2S162.
ModBase	Search...
Protein-protein interaction databases
STRING	B2S162.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000006378; EBBORP00000005802; EBBORG00000006378.
GeneID	6276606.
GenomeReviews	Gene locus BH0720 in contig CP000048_GR.
KEGG	bhr:BH0720.
PATRIC	20568820. VBIBorHer61105_0724.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007426.
HOGENOM	HBG352811.
OMA	MIIRNIL.
ProtClustDB	PRK12305.
Enzyme and pathway databases
BioCyc	BHER314723:BH0720-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BORHD

Accession

Primary (citable) accession number: B2S162

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program