Reviewed, UniProtKB/Swiss-Prot B2S140 (TRMD_BORHD)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BH0698

Organism

Borrelia hermsii (strain DAH) [Complete proteome] [HAMAP]

Taxonomic identifier

314723 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000130138
Regions
	Region	130 – 135	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	110	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2S140-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: F99AC8119D9F9F1D	FASTA	239	27,048
	10 20 30 40 50 60 MKITILSLFP SIITPFFENS IMKKVVDRGI ISYEVISIRD FSDDKHKRCD DIPYGGGAGM 70 80 90 100 110 120 VLKAPPISAA LEYVNAKSKT TIFVSPSGLK YTQKLAYDLS KKSELVIICG RYEGLDQRVI 130 140 150 160 170 180 DLYVDLEVSV GDYVLSSGEV AALVIIDSVY RLLDGAINPS SLLEESFSFD CGLLEYPHYT 190 200 210 220 230 RPYEFKGLEV PDVLLSGHHE EIKKWRFIKS VEKTKKNRYD LYLKYLEMIG ENDGFSKKN

References

[1]

"The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever." Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000048 Genomic DNA. Translation: AAX17196.1.
RefSeq	YP_001884116.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6276625.
GenomeReviews	Gene locus BH0698 in contig CP000048_GR.
KEGG	bhr:BH0698.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HRSVDDT.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BORHD

Accession

Primary (citable) accession number: B2S140

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)