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B2S0T6 (SYM_BORHD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:BH0587
OrganismBorrelia hermsii (strain DAH) [Complete proteome] [HAMAP]
Taxonomic identifier314723 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm By similarity HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Methionine--tRNA ligase HAMAP MF_00098
PRO_1000093698

Regions

Domain565 – 670106tRNA-binding
Motif12 – 2211"HIGH" region HAMAP MF_00098
Motif330 – 3345"KMSKS" region HAMAP MF_00098

Sites

Metal binding1431Zinc By similarity
Metal binding1461Zinc By similarity
Metal binding1551Zinc By similarity
Metal binding1581Zinc By similarity
Binding site3331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2S0T6 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 6511405030DF4893

FASTA72984,614
        10         20         30         40         50         60 
MKKKNLITAA LPYVNNIPHL GNLVQVLSAD AFARYSRMMG IETLYVCGTD EYGTATETKA 

        70         80         90        100        110        120 
LIEKTTPEEL CNKYYEIHKS IYEWFNIKFD IFGRTTNKRH KETVQDLFLK LEKNGYTTEK 

       130        140        150        160        170        180 
ESEQFFCKQD QMFLADRYVT GECPNCGNNA KGDQCENCSK LLSPTDLINP KCIICKNIPI 

       190        200        210        220        230        240 
IKTTKHIYID LPKIKDELSH WMQITELNTN WNTNAIKITN AFLRDGLKER AITRDLKWGI 

       250        260        270        280        290        300 
PVPKKEYENK VFYVWFDAPI GYISITKEIS KDWESWWKNN EETNLVQFIG KDNILFHTVI 

       310        320        330        340        350        360 
FPSIALGSKE NWTMLSKLAS SEYLNYENLK FSKSAGTGIF GNDVITTGIP SDIWRFYIYY 

       370        380        390        400        410        420 
NRPEKSDFQF MWDDFMERVN SELIGNFSNL VNRVLTFYKK FFGDQIDKIE IKEDFWQEIN 

       430        440        450        460        470        480 
LKYDKTLNFF KQVELKSALK EILDISRTGN KIFQDKEPWK TKDSTPQKTK ELLLNLIYLI 

       490        500        510        520        530        540 
RDLSILISPF IPHTSDKIRR FFGNSYEISN RFLGTNLGLS TIQFTEVLFI KLEKHLINSL 

       550        560        570        580        590        600 
KLKYSGSKNM QDEQTSNQIN PINLFSEQVC LKVVQVKTIE RNPDAEKLFI LKLNDGSSDG 

       610        620        630        640        650        660 
KQIVSSLADY YKEEELIGKH IIIVDNLKPA KFRGIKSEGM LIATEDKDKN FKVIIVEDFK 

       670        680        690        700        710        720 
DNPIPGERII LESDSYKELK SPAKISIDKF FKTQIVAENG ELKINGINLI LEHSKEKILS 


IEIPNGKVY

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References

[1]"The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DAH.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000048 Genomic DNA. Translation: AAX17092.1.
RefSeqYP_001884012.1. NC_010673.1.

3D structure databases

ProteinModelPortalB2S0T6.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2S0T6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBORT00000006958; EBBORP00000006382; EBBORG00000006958.
GeneID6277009.
GenomeReviewsGene locus BH0587 in contig CP000048_GR.
KEGGbhr:BH0587.
PATRIC20568530. VBIBorHer61105_0589.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000007495.
HOGENOMHBG721667.
OMAEEKTRNV.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycBHER314723:BH0587-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BORHD
AccessionPrimary (citable) accession number: B2S0T6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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