B2S0H3 (LSPA_BORHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BH0469

Organism

Borrelia hermsii (strain DAH) [Complete proteome] [HAMAP]

Taxonomic identifier

314723 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000097232
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	72 – 92	21	Helical; Potential
	Transmembrane	93 – 113	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
Sites
	Active site	114	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2S0H3 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 58862D230525394C	FASTA	170	19,509
	10 20 30 40 50 60 MNIDRNRLIG SVIFIFILVF IDQWSKYLVV KYISIGTEYL SFFGDFFKII HVRNTGILFS 70 80 90 100 110 120 IGANINSSLK NLFFLVIPII ILVFVFCFIL KETNKIARIA LILILSGGIG NIIDRLFRPL 130 140 150 160 170 GVVDFLDVKF FGIFGLQRWP TFNFADSYVV IGITLFIIYD LFAKNKSTDL

References

[1]

"The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever." Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DAH.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000048 Genomic DNA. Translation: AAX16979.1.
RefSeq	YP_001883899.1. NC_010673.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2S0H3.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000006435; EBBORP00000005859; EBBORG00000006435.
GeneID	6276788.
GenomeReviews	Gene locus BH0469 in contig CP000048_GR.
KEGG	bhr:BH0469.
PATRIC	20568292. VBIBorHer61105_0474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007459.
HOGENOM	HBG724422.
OMA	ITLFIIY.
ProtClustDB	PRK01574.
Enzyme and pathway databases
BioCyc	BHER314723:BH0469-MONOMER.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BORHD

Accession

Primary (citable) accession number: B2S0H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program