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B2RZW1 (SYL_BORHD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BH0251
OrganismBorrelia hermsii (strain HS1 / DAH) [Complete proteome] [HAMAP]
Taxonomic identifier314723 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia

Protein attributes

Sequence length842 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 842842Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091293

Regions

Motif44 – 5512"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2RZW1 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 6826EF84209307EA

FASTA84298,521
        10         20         30         40         50         60 
MSEYNFTKIE KKWQDYWDKH KTYKVNEDPN IPKEKRIYIL DMFPYPSANG LHVGHPEGYT 

        70         80         90        100        110        120 
ATDILTRYKL LNGFNVLHPI GFDSFGLPAE NYAIQTGEHP KKITEKNIKK FKEQIKALGF 

       130        140        150        160        170        180 
AYDWDREIRT HDENYYKWTQ WIFLKLYKKG LAYTKEMPVW YCPDLGTVLS NEEVIQTPDG 

       190        200        210        220        230        240 
PRSERGFHKV KRKPLRQWIL KITEYAERLI KDLEEIDWPE SVKEMQKNWI GKSTGAEIEF 

       250        260        270        280        290        300 
SVKASKEKIK VFTTRPDTIF GVTYLVLAPE HSIVDKITKD EFKPMIVEYR ERETLKSDLE 

       310        320        330        340        350        360 
RTSLEKDKTG VFTGAYAINP ITGEEIPIWI GSYILGTYGT GAVMSVPAHD ARDFEFAKKY 

       370        380        390        400        410        420 
NLPIKQVVSQ TGNNEILTQP FTENGISINT PEEFNNLKTD EIKERVIKWL TENKKGQKKV 

       430        440        450        460        470        480 
NYKLRDWIFS RQRYWGEPIP IILDDDLNEI PLEEDELPLR LPEIENYKPS GTGESPLSRI 

       490        500        510        520        530        540 
QDWVNIKRNG KTYKRETNTM PQWAGSCWYY IRYLDPNNDN EFASKEKINY WMPVDLYIGG 

       550        560        570        580        590        600 
AEHSVLHLLY ARFWHKVLYD LGYVNTKEPF KKLINQGMIT SFAYQDENGI LIPNDEVEKK 

       610        620        630        640        650        660 
DNKFFSKKNN KELKQIVAKM SKSLKNIINP DDIIKEYGAD SMRIYEMFMG PLTDSKPWNT 

       670        680        690        700        710        720 
QGLIGIFRFL NKIWTIKNKE LTKESASKEI ISELHKTIKK VTEDIENLNF NTAISSLMIF 

       730        740        750        760        770        780 
INELLKHDKN YLEIFKPLTI ILSPFAPHLG EELWEYMGET PSIFKSAKWP EYDPNLIIDN 

       790        800        810        820        830        840 
TREIVLQING KIKDKIILNK GTNEEALKSI ALKNHKIMQN IQNKQIIKII TVKDKLINIV 


TR 

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References

[1]"The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HS1 / DAH.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000048 Genomic DNA. Translation: AAX16767.1.
RefSeqYP_001883687.1. NC_010673.1.

3D structure databases

ProteinModelPortalB2RZW1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314723.BH0251.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAX16767; AAX16767; BH0251.
GeneID6277013.
KEGGbhr:BH0251.
PATRIC20567826. VBIBorHer61105_0251.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAERYWMNV.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBHER314723:GJES-246-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BORHD
AccessionPrimary (citable) accession number: B2RZW1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries