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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Borrelia hermsii (strain HS1 / DAH)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBHER314723:GJES-246-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BH0251
OrganismiBorrelia hermsii (strain HS1 / DAH)
Taxonomic identifieri314723 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000008834: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 842842Leucine--tRNA ligasePRO_1000091293Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi314723.BH0251.

Structurei

3D structure databases

ProteinModelPortaliB2RZW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi44 – 5512"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2RZW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYNFTKIE KKWQDYWDKH KTYKVNEDPN IPKEKRIYIL DMFPYPSANG
60 70 80 90 100
LHVGHPEGYT ATDILTRYKL LNGFNVLHPI GFDSFGLPAE NYAIQTGEHP
110 120 130 140 150
KKITEKNIKK FKEQIKALGF AYDWDREIRT HDENYYKWTQ WIFLKLYKKG
160 170 180 190 200
LAYTKEMPVW YCPDLGTVLS NEEVIQTPDG PRSERGFHKV KRKPLRQWIL
210 220 230 240 250
KITEYAERLI KDLEEIDWPE SVKEMQKNWI GKSTGAEIEF SVKASKEKIK
260 270 280 290 300
VFTTRPDTIF GVTYLVLAPE HSIVDKITKD EFKPMIVEYR ERETLKSDLE
310 320 330 340 350
RTSLEKDKTG VFTGAYAINP ITGEEIPIWI GSYILGTYGT GAVMSVPAHD
360 370 380 390 400
ARDFEFAKKY NLPIKQVVSQ TGNNEILTQP FTENGISINT PEEFNNLKTD
410 420 430 440 450
EIKERVIKWL TENKKGQKKV NYKLRDWIFS RQRYWGEPIP IILDDDLNEI
460 470 480 490 500
PLEEDELPLR LPEIENYKPS GTGESPLSRI QDWVNIKRNG KTYKRETNTM
510 520 530 540 550
PQWAGSCWYY IRYLDPNNDN EFASKEKINY WMPVDLYIGG AEHSVLHLLY
560 570 580 590 600
ARFWHKVLYD LGYVNTKEPF KKLINQGMIT SFAYQDENGI LIPNDEVEKK
610 620 630 640 650
DNKFFSKKNN KELKQIVAKM SKSLKNIINP DDIIKEYGAD SMRIYEMFMG
660 670 680 690 700
PLTDSKPWNT QGLIGIFRFL NKIWTIKNKE LTKESASKEI ISELHKTIKK
710 720 730 740 750
VTEDIENLNF NTAISSLMIF INELLKHDKN YLEIFKPLTI ILSPFAPHLG
760 770 780 790 800
EELWEYMGET PSIFKSAKWP EYDPNLIIDN TREIVLQING KIKDKIILNK
810 820 830 840
GTNEEALKSI ALKNHKIMQN IQNKQIIKII TVKDKLINIV TR
Length:842
Mass (Da):98,521
Last modified:July 1, 2008 - v1
Checksum:i6826EF84209307EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000048 Genomic DNA. Translation: AAX16767.1.
RefSeqiYP_001883687.1. NC_010673.1.

Genome annotation databases

EnsemblBacteriaiAAX16767; AAX16767; BH0251.
GeneIDi6277013.
KEGGibhr:BH0251.
PATRICi20567826. VBIBorHer61105_0251.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000048 Genomic DNA. Translation: AAX16767.1.
RefSeqiYP_001883687.1. NC_010673.1.

3D structure databases

ProteinModelPortaliB2RZW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi314723.BH0251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX16767; AAX16767; BH0251.
GeneIDi6277013.
KEGGibhr:BH0251.
PATRICi20567826. VBIBorHer61105_0251.

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBHER314723:GJES-246-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
    Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HS1 / DAH.

Entry informationi

Entry nameiSYL_BORHD
AccessioniPrimary (citable) accession number: B2RZW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: March 4, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.