Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Borrelia hermsii (strain HS1 / DAH)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi298Magnesium or manganese 1UniRule annotation1
Metal bindingi312Magnesium or manganese 1UniRule annotation1
Metal bindingi312Magnesium or manganese 2UniRule annotation1
Metal bindingi314Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 223ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:BH0200
OrganismiBorrelia hermsii (strain HS1 / DAH)
Taxonomic identifieri314723 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorrelia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000911621 – 365D-alanine--D-alanine ligaseAdd BLAST365

Proteomic databases

PRIDEiB2RZR0

Structurei

3D structure databases

ProteinModelPortaliB2RZR0
SMRiB2RZR0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 345ATP-graspUniRule annotationAdd BLAST211

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiYETKYTE
OrthoDBiPOG091H06GK

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

B2RZR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKKNLMLIF GGVSFEHEIS LRSAYGIYSA LMKLDKYNLY PSFIDKITGI
60 70 80 90 100
WYLLDSVPDA PELIKRDSSA IISLIPGCGI FVNDEDLEID VVFPIVHGRT
110 120 130 140 150
GEDGAIQGFL KMMDIPCVGA GILGSAISIN KYFCKLLLKS FNIPLVPFIG
160 170 180 190 200
FRKYDYFLDK EGIKKDIKQS LNYPVIVKPA MLGSSIGISI AYNDTQIEKC
210 220 230 240 250
IEEAFEYDLT VVVEKFMKVR EIECSVIGNE QIKIFTPGEI VVQDFVFYDY
260 270 280 290 300
DAKYSTIPGN SVVFNIPARL DMKHLLDIKE YAFLTYKCLE LRGMARIDFL
310 320 330 340 350
IEKDTNLIYV NEINTIPGFT DISMFSKMCE HDGLDYESLV DNLVSLAFQS
360
YAKRKERIDF KRLEN
Length:365
Mass (Da):41,559
Last modified:July 1, 2008 - v1
Checksum:iFFC7A489AF0D8A86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000048 Genomic DNA Translation: AAX16716.1

Genome annotation databases

EnsemblBacteriaiAAX16716; AAX16716; BH0200
KEGGibhr:BH0200

Similar proteinsi

Entry informationi

Entry nameiDDL_BORHD
AccessioniPrimary (citable) accession number: B2RZR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health