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Protein

Vacuolar protein sorting-associated protein 29

Gene

Vps29

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with ANKRD27 (By similarity).By similarity

Caution

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Zinc 1By similarity1
Metal bindingi10Zinc 1By similarity1
Metal bindingi39Zinc 1By similarity1
Metal bindingi39Zinc 2By similarity1
Metal bindingi62Zinc 2By similarity1
Metal bindingi86Zinc 2By similarity1
Metal bindingi115Zinc 2By similarity1
Metal bindingi117Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-3238698 WNT ligand biogenesis and trafficking

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 29By similarity
Alternative name(s):
Vesicle protein sorting 29By similarity
Gene namesi
Name:Vps29Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1308332 Vps29

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003651041 – 182Vacuolar protein sorting-associated protein 29Add BLAST182

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiB2RZ78
PeptideAtlasiB2RZ78
PRIDEiB2RZ78

PTM databases

iPTMnetiB2RZ78
PhosphoSitePlusiB2RZ78

Expressioni

Gene expression databases

BgeeiENSRNOG00000001274
ExpressionAtlasiB2RZ78 baseline and differential
GenevisibleiB2RZ78 RN

Interactioni

Subunit structurei

Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35. The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform. The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants. The originally described retromer complex (also called SNX-BAR retromer) is a pentamer containing the CSC and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to form the SNX27-retromer complex. Interacts with VPS26A, VPS35, SNX1, SNX2, SNX27, WASHC5, TBC1D5, VPS26B and ANKRD27 (By similarity).By similarity

Protein-protein interaction databases

BioGridi2526471 interactor.
STRINGi10116.ENSRNOP00000001717

Structurei

3D structure databases

SMRiB2RZ78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS29 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG3325 Eukaryota
COG0622 LUCA
GeneTreeiENSGT00390000012669
HOGENOMiHOG000293431
HOVERGENiHBG056165
InParanoidiB2RZ78
KOiK18467
PhylomeDBiB2RZ78

Family and domain databases

CDDicd07394 MPP_Vps29, 1 hit
Gene3Di3.60.21.101 hit
InterProiView protein in InterPro
IPR024654 Calcineurin-like_PHP_lpxH
IPR029052 Metallo-depent_PP-like
IPR000979 Phosphodiesterase_MJ0936/Vps29
IPR028661 Vps29
PANTHERiPTHR11124 PTHR11124, 1 hit
PTHR11124:SF12 PTHR11124:SF12, 1 hit
PfamiView protein in Pfam
PF12850 Metallophos_2, 1 hit
TIGRFAMsiTIGR00040 yfcE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B2RZ78-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVLVLGDLH IPHRCNSLPA KFKKLLVPGK IQHILCTGNL CTKESYDYLK
60 70 80 90 100
TLAGDVHIVR GDFDESLNYP EQKVVTVGQF KIGLIHGHQV IPWGDMASLA
110 120 130 140 150
LLQRQFDVDI LISGHTHKFE AFEHENKFYI NPGSATGAYN ALETNIIPSF
160 170 180
VLMDIQASTV VTYVYQLIGD DVKVERIEYK KS
Length:182
Mass (Da):20,469
Last modified:March 3, 2009 - v2
Checksum:iCCDCC36B7B9EC64E
GO
Isoform 21 Publication (identifier: B2RZ78-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAGHR

Note: No experimental confirmation available.Curated
Show »
Length:186
Mass (Da):20,890
Checksum:iDAA50C6A39F400D9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0530301M → MAGHR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473973 Genomic DNA Translation: EDM13693.1
BC167055 mRNA Translation: AAI67055.1
RefSeqiNP_001099402.1, NM_001105932.1 [B2RZ78-1]
XP_006249424.1, XM_006249362.3 [B2RZ78-2]
UniGeneiRn.2547

Genome annotation databases

EnsembliENSRNOT00000001717; ENSRNOP00000001717; ENSRNOG00000001274 [B2RZ78-1]
ENSRNOT00000088905; ENSRNOP00000074640; ENSRNOG00000001274 [B2RZ78-2]
GeneIDi288666
KEGGirno:288666
UCSCiRGD:1308332 rat [B2RZ78-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVPS29_RAT
AccessioniPrimary (citable) accession number: B2RZ78
Secondary accession number(s): A6J1A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 3, 2009
Last modified: March 28, 2018
This is version 72 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome