B2RZ78 (VPS29_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 29
EC=3.1.3.3

Alternative name(s):
Vesicle protein sorting 29

Gene names

Name:

Vps29

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro) By similarity. UniProtKB Q9UBQ0
Catalytic activity	O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate. UniProtKB Q9UBQ0
Cofactor	Binds 2 zinc ions per subunit By similarity. UniProtKB Q9QZ88
Subunit structure	Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN By similarity. Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2 By similarity. UniProtKB Q9UBQ0
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Endosome membrane; Peripheral membrane protein By similarity UniProtKB Q9UBQ0 UniProtKB Q9QZ88.
Sequence similarities	Belongs to the VPS29 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome Membrane
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoserine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.2 (identifier: B2RZ78-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: B2RZ78-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAGHR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Vacuolar protein sorting-associated protein 29		PRO_0000365104
Sites
	Metal binding	8	1	Zinc 1 By similarity UniProtKB Q9QZ88
	Metal binding	10	1	Zinc 1 By similarity UniProtKB Q9QZ88
	Metal binding	39	1	Zinc 1 By similarity UniProtKB Q9QZ88
	Metal binding	39	1	Zinc 2 By similarity UniProtKB Q9QZ88
	Metal binding	62	1	Zinc 2 By similarity UniProtKB Q9QZ88
	Metal binding	86	1	Zinc 2 By similarity UniProtKB Q9QZ88
	Metal binding	115	1	Zinc 2 By similarity UniProtKB Q9QZ88
	Metal binding	117	1	Zinc 1 By similarity UniProtKB Q9QZ88
Amino acid modifications
	Modified residue	50	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	1	1	M → MAGHR in isoform 2. Ref.2		VSP_053030

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: CCDCC36B7B9EC64E	FASTA	182	20,469
	10 20 30 40 50 60 MLVLVLGDLH IPHRCNSLPA KFKKLLVPGK IQHILCTGNL CTKESYDYLK TLAGDVHIVR 70 80 90 100 110 120 GDFDESLNYP EQKVVTVGQF KIGLIHGHQV IPWGDMASLA LLQRQFDVDI LISGHTHKFE 130 140 150 160 170 180 AFEHENKFYI NPGSATGAYN ALETNIIPSF VLMDIQASTV VTYVYQLIGD DVKVERIEYK KS
	Isoform 2 [UniParc]. Checksum: DAA50C6A39F400D9	FASTA	186	20,890
	10 20 30 40 50 60 MAGHRLVLVL GDLHIPHRCN SLPAKFKKLL VPGKIQHILC TGNLCTKESY DYLKTLAGDV 70 80 90 100 110 120 HIVRGDFDES LNYPEQKVVT VGQFKIGLIH GHQVIPWGDM ASLALLQRQF DVDILISGHT 130 140 150 160 170 180 HKFEAFEHEN KFYINPGSAT GAYNALETNI IPSFVLMDIQ ASTVVTYVYQ LIGDDVKVER IEYKKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Ovary.
[3]	Maurya D.K., Bhargava P. Submitted (DEC-2008) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY (ISOFORM 1).

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH473973 Genomic DNA. Translation: EDM13693.1. BC167055 mRNA. Translation: AAI67055.1.
IPI	IPI00870884. IPI00923130.
RefSeq	NP_001099402.1. NM_001105932.1.
UniGene	Rn.2547.
3D structure databases
ProteinModelPortal	B2RZ78.
ModBase	Search...
Protein-protein interaction databases
STRING	B2RZ78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001717; ENSRNOP00000001717; ENSRNOG00000001274.
GeneID	288666.
KEGG	rno:288666.
Organism-specific databases
CTD	51699.
RGD	1308332. Vps29.
Phylogenomic databases
eggNOG	maNOG06426.
GeneTree	ENSGT00390000012669.
HOVERGEN	HBG056165.
OrthoDB	EOG49PB0G.
Gene expression databases
Genevestigator	B2RZ78.
Family and domain databases
InterPro	IPR024654. Calcineurin-like_PEstase_dom. IPR000979. Phosphodiesterase_MJ0936. [Graphical view]
KO	K07095.
PANTHER	PTHR11124. PTHR11124. 1 hit.
Pfam	PF12850. Metallophos_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00040. YfcE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VPS29_RAT

Accession

Primary (citable) accession number: B2RZ78
Secondary accession number(s): A6J1A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	November 16, 2011
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program