B2RZ50 (CDKN3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase inhibitor 3
EC=3.1.3.16
EC=3.1.3.48

Alternative name(s):
CDK2-associated dual-specificity phosphatase
Kinase-associated phosphatase

Gene names

Name:	Cdkn3
Synonyms:	Kap

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner By similarity. UniProtKB Q16667
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. UniProtKB Q16667 A phosphoprotein + H2O = a protein + phosphate. UniProtKB Q16667
Subunit structure	Interacts with cyclin-dependent kinases such as CDK1, CDK2 and CDK3. Does not interact with CDK4. Interacts (via C-terminus) with phosphorylated CDK2 (via C-terminal helix). Interacts with MS4A3 (via C-terminus); the interaction enhances CDKN3 enzymatic activity By similarity. UniProtKB Q16667
Subcellular location	Cytoplasm › perinuclear region By similarity UniProtKB Q16667.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle arrest Inferred from electronic annotation. Source: Compara
Cellular_component	perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: Compara protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Cyclin-dependent kinase inhibitor 3		PRO_0000396639
Regions
	Region	1 – 34	34	Interaction with CDK2 By similarity UniProtKB Q16667
Sites
	Active site	140	1	By similarity UniProtKB Q16667

Sequences

	Sequence		Length	Mass (Da)
	B2RZ50 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 345F42838AA50801	FASTA	212	23,774
	10 20 30 40 50 60 MKPPISIQAS EFDSSDEEPA DDEQTPIQIS WLPLSRVNCS QFLGLCALPG CKFKDVRRNI 70 80 90 100 110 120 QKDTEELKSS GIQDVFVFCT RGELSKYRVP NLLDLYQQYG IVTHHHPIPD GGTPDIGSCW 130 140 150 160 170 180 EIMEELATCL KNNRKTLIHC YGGLGRSCLV AACLLLYLSD SISPQQAIDS LRDVRGSGAI 190 200 210 QTIKQYNYLH EFRDKLAAYL SSRDSLSRSV SR

References

[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Brown Norway/NHsdMcwi. Tissue: Embryonic liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH474040 Genomic DNA. Translation: EDL88327.1. BC167026 mRNA. Translation: AAI67026.1.
IPI	IPI00870662.
UniGene	Rn.229034.
3D structure databases
ProteinModelPortal	B2RZ50.
SMR	B2RZ50. Positions 21-203.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000012985.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000012985; ENSRNOP00000012985; ENSRNOG00000009785.
Organism-specific databases
RGD	1307781. Cdkn3.
Phylogenomic databases
eggNOG	COG2453.
GeneTree	ENSGT00390000004717.
HOGENOM	HOG000060336.
HOVERGEN	HBG075798.
OrthoDB	EOG49W2GK.
Gene expression databases
Genevestigator	B2RZ50.
Family and domain databases
InterPro	IPR008425. CDK_inhib_3. IPR022778. CDKN3. IPR000387. Tyr/Dual-sp_Pase. [Graphical view]
Pfam	PF05706. CDKN3. 1 hit. [Graphical view]
PIRSF	PIRSF037322. CDKN3. 1 hit.
PROSITE	PS50056. TYR_PHOSPHATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDKN3_RAT

Accession

Primary (citable) accession number: B2RZ50

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	July 1, 2008
Last modified:	April 3, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program