Reviewed, UniProtKB/Swiss-Prot B2RYN7 (SPAST_RAT)

Names and origin

Protein names

Recommended name:
    Spastin
    EC=3.6.4.3

Gene names

Name:	Spast
Synonyms:	Spg4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis By similarity. Plays a role in axon growth and the formation of axonal branches.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. Binding to ATP stabilizes the homohexameric form. Binds to microtubules at least in part via the alpha-tubulin and beta-tubulin tails. The hexamer may adopt a ring conformation through which microtubules pass prior to being severed. Does not interact strongly with tubulin heterodimers. Interacts (via MIT domain) with CHMP1B; the interaction is direct. Interacts with ATL1, RTN1, SSNA1 and ZFYVE27 By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Endoplasmic reticulum By similarity. Endosome By similarity. Nucleus By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Note: Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Endoplasmic reticulum Endosome Membrane Microtubule Nucleus
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis, completion of separation Inferred from sequence or structural similarity. Source: UniProtKB microtubule bundle formation Inferred from sequence or structural similarity. Source: UniProtKB microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB nervous system development Inferred from electronic annotation. Source: UniProtKB-KW protein hexamerization Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alpha-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 581	581	Spastin		PRO_0000367135
Regions
	Transmembrane	55 – 75	21	Potential
	Domain	117 – 192	76	MIT
	Nucleotide binding	347 – 354	8	ATP Potential
	Region	1 – 265	265	Required for interaction with RTN1 By similarity
	Region	1 – 191	191	Required for midbody localization By similarity
	Region	1 – 78	78	Required for interaction with ATL1 By similarity
	Region	1 – 48	48	Required for nuclear localization By similarity
	Region	48 – 85	38	Required for interaction with SSNA1 and microtubules By similarity
	Region	110 – 193	84	Sufficient for interaction with CHMP1B By similarity
	Region	111 – 194	84	Required for interaction with microtubules By similarity
	Region	194 – 581	388	Sufficient for microtubule severing By similarity
	Region	194 – 293	100	Sufficient for interaction with microtubules By similarity
	Region	235 – 293	59	Required for interaction with microtubules and microtubule severing By similarity
	Motif	4 – 11	8	Nuclear localization signal By similarity
	Motif	57 – 65	9	Nuclear export signal By similarity
	Motif	274 – 277	4	Nuclear localization signal By similarity
Amino acid modifications
	Modified residue	233	1	Phosphoserine By similarity
	Modified residue	268	1	Phosphothreonine By similarity
	Modified residue	271	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2RYN7-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: A404A3B676F9F224	FASTA	581	63,022
	10 20 30 40 50 60 MSSPAGRRKK KGSGGASPAP ARPPPPAAVP APAAGPAPAP GSPHKRNLYY FSYPLVVGFA 70 80 90 100 110 120 LLRLLACHLG LLFVWLCQRF SRALMAAKRS SGTAPAPASP STPAPGPGGE AESVRVFHKQ 130 140 150 160 170 180 AFEYISIALR IDEEEKGQKE QAVEWYKKGI EELEKGIAVI VTGQGEQYER ARRLQAKMMT 190 200 210 220 230 240 NLVMAKDRLQ LLESGAVPKK KDPLTHASNS LPRSKTVMKS GSTGLSGHHR APSCSGLSMV 250 260 270 280 290 300 SGARPGSGPA ATTHKGTSKP NRTNKPSTPT TAVRKKKDLK NFRNVDSNLA NLIMNEIVDN 310 320 330 340 350 360 GTAVKFDDIA GQELAKQALQ EIVILPSLRP ELFTGLRAPA RGLLLFGPPG NGKTMLAKAV 370 380 390 400 410 420 AAESNATFFN ISAASLTSKY VGEGEKLVRA LFAVARELQP SIIFIDEVDS LLCERREGEH 430 440 450 460 470 480 DASRRLKTEF LIEFDGVQSA GDDRVLVMGA TNRPQELDEA VLRRFIKRVY VSLPNEETRL 490 500 510 520 530 540 LLLKNLLCKQ GSPLTQKELA QLARMTDGYS GSDLTALAKD AALGPIRELK PEQVKNMSAS 550 560 570 580 EMRNIRLSDF TESLKKIKRS VSPQTLEAYI RWNKDFGDTT V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[2]	"The microtubule-severing proteins spastin and katanin participate differently in the formation of axonal branches." Yu W., Qiang L., Solowska J.M., Karabay A., Korulu S., Baas P.W. Mol. Biol. Cell 19:1485-1498(2008) [PubMed: 18234839] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC166846 mRNA. Translation: AAI66846.1.
IPI	IPI00365573.
UniGene	Rn.41645
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2RYN7.
Organism-specific databases
RGD	1308494. Spast.
Gene expression databases
Genevestigator	B2RYN7.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. IPR017179. Spastin. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PIRSF	PIRSF037338. Spastin. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_RAT

Accession

Primary (citable) accession number: B2RYN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	October 13, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)