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Protein

Lys-63-specific deubiquitinase BRCC36

Gene

Brcc3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Zinc; catalyticBy similarity
Metal bindingi124 – 1241Zinc; catalyticBy similarity
Metal bindingi135 – 1351Zinc; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA repair, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-RNO-5693571. Nonhomologous End-Joining (NHEJ).
R-RNO-5693607. Processing of DNA double-strand break ends.
R-RNO-69473. G2/M DNA damage checkpoint.

Protein family/group databases

MEROPSiM67.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Lys-63-specific deubiquitinase BRCC36 (EC:3.4.19.-By similarity)
Alternative name(s):
BRCA1-A complex subunit BRCC36
BRCA1/BRCA2-containing complex subunit 3
BRCA1/BRCA2-containing complex subunit 36
BRISC complex subunit BRCC36
Gene namesi
Name:Brcc3
Synonyms:Brcc36
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1588543. Brcc3.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cytoplasmcytoskeletonspindle pole By similarity

  • Note: Localizes at sites of DNA damage at double-strand breaks (DSBs). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 291290Lys-63-specific deubiquitinase BRCC36PRO_0000373946Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei233 – 2331PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiB2RYM5.
PeptideAtlasiB2RYM5.
PRIDEiB2RYM5.

PTM databases

iPTMnetiB2RYM5.
PhosphoSiteiB2RYM5.

Expressioni

Gene expression databases

BgeeiENSRNOG00000048061.
GenevisibleiB2RYM5. RN.

Interactioni

Subunit structurei

Component of the ARISC complex, at least composed of UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas and BRE/BRCC45. Component of the BRISC complex, at least composed of FAM175B/ABRO1, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. Identified in a complex with SHMT2 and the other subunits of the BRISC complex. In the BRISC complex, interacts directly with FAM175B. Identified in a complex with FAM175B and NUMA1. The BRISC complex interacts with the CSN complex. Component of the BRCA1/BRCA2 containing complex (BRCC), which also contains BRCA1, BRCA2, BARD1, BRE and RAD51. BRCC is a ubiquitin E3 ligase complex that enhances cellular survival following DNA damage. Interacts with BRCA1. Binds polyubiquitin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi261425. 1 interaction.
STRINGi10116.ENSRNOP00000066438.

Structurei

3D structure databases

ProteinModelPortaliB2RYM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 148142MPNAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi122 – 13514JAMM motifAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M67A family. BRCC36 subfamily.Curated
Contains 1 MPN (JAB/Mov34) domain.Curated

Phylogenomic databases

eggNOGiKOG1555. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00390000000360.
InParanoidiB2RYM5.
KOiK11864.
OMAiDSKFTYT.
OrthoDBiEOG091G0MM4.
PhylomeDBiB2RYM5.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2RYM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPVVQAVQ AVHLESDAFL VCLNHALSTE KEEVMGLCIG ELNDDVRSES
60 70 80 90 100
KFAHAGSDVC TVPEKVDSIR VVHIHSVIIL RRSDKRKDRV EISPEQLSAA
110 120 130 140 150
STEAERLAEL TGRPMRVVGW YHSHPHITVW PSHVDVRTQA MYQMMDQGFV
160 170 180 190 200
GLIFSCFIED KNTKTGRVLY TCFQSVQAQK SSDYERIEIP VHVVPHVTIG
210 220 230 240 250
KVCLESAVEL PKILCQEEQD AYRRIHSLTH LDSVTKIHNG SVFTKNLCSQ
260 270 280 290
MSAVSGPLLQ WLEDRLEQNQ QHLRELQREK EELMAELRSL E
Length:291
Mass (Da):33,016
Last modified:July 1, 2008 - v1
Checksum:i93EB2A7643C70F36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474092 Genomic DNA. Translation: EDL83574.1.
BC166833 mRNA. Translation: AAI66833.1.
RefSeqiNP_001120772.1. NM_001127300.1.
UniGeneiRn.52727.

Genome annotation databases

EnsembliENSRNOT00000071068; ENSRNOP00000066438; ENSRNOG00000048061.
GeneIDi316794.
KEGGirno:316794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474092 Genomic DNA. Translation: EDL83574.1.
BC166833 mRNA. Translation: AAI66833.1.
RefSeqiNP_001120772.1. NM_001127300.1.
UniGeneiRn.52727.

3D structure databases

ProteinModelPortaliB2RYM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi261425. 1 interaction.
STRINGi10116.ENSRNOP00000066438.

Protein family/group databases

MEROPSiM67.004.

PTM databases

iPTMnetiB2RYM5.
PhosphoSiteiB2RYM5.

Proteomic databases

PaxDbiB2RYM5.
PeptideAtlasiB2RYM5.
PRIDEiB2RYM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000071068; ENSRNOP00000066438; ENSRNOG00000048061.
GeneIDi316794.
KEGGirno:316794.

Organism-specific databases

CTDi79184.
RGDi1588543. Brcc3.

Phylogenomic databases

eggNOGiKOG1555. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00390000000360.
InParanoidiB2RYM5.
KOiK11864.
OMAiDSKFTYT.
OrthoDBiEOG091G0MM4.
PhylomeDBiB2RYM5.

Enzyme and pathway databases

ReactomeiR-RNO-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-RNO-5693571. Nonhomologous End-Joining (NHEJ).
R-RNO-5693607. Processing of DNA double-strand break ends.
R-RNO-69473. G2/M DNA damage checkpoint.

Miscellaneous databases

PROiB2RYM5.

Gene expression databases

BgeeiENSRNOG00000048061.
GenevisibleiB2RYM5. RN.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRCC3_RAT
AccessioniPrimary (citable) accession number: B2RYM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 1, 2008
Last modified: September 7, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.