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B2RYM5

- BRCC3_RAT

UniProt

B2RYM5 - BRCC3_RAT

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Protein

Lys-63-specific deubiquitinase BRCC36

Gene

Brcc3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Zinc; catalyticBy similarity
Metal bindingi124 – 1241Zinc; catalyticBy similarity
Metal bindingi135 – 1351Zinc; catalyticBy similarity

GO - Molecular functioni

  1. enzyme regulator activity Source: Ensembl
  2. metal ion binding Source: UniProtKB-KW
  3. metallopeptidase activity Source: UniProtKB
  4. polyubiquitin binding Source: UniProtKB
  5. ubiquitin-specific protease activity Source: UniProtKB
  6. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. double-strand break repair Source: UniProtKB
  2. G2 DNA damage checkpoint Source: UniProtKB
  3. histone H2A K63-linked deubiquitination Source: UniProtKB
  4. positive regulation of DNA repair Source: UniProtKB
  5. protein K63-linked deubiquitination Source: UniProtKB
  6. response to ionizing radiation Source: UniProtKB
  7. response to X-ray Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM67.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Lys-63-specific deubiquitinase BRCC36 (EC:3.4.19.-)
Alternative name(s):
BRCA1-A complex subunit BRCC36
BRCA1/BRCA2-containing complex subunit 3
BRCA1/BRCA2-containing complex subunit 36
BRISC complex subunit BRCC36
Gene namesi
Name:Brcc3
Synonyms:Brcc36
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi1588543. Brcc3.

Subcellular locationi

Nucleus By similarity
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).By similarity

GO - Cellular componenti

  1. BRCA1-A complex Source: UniProtKB
  2. BRISC complex Source: UniProtKB
  3. nuclear ubiquitin ligase complex Source: Ensembl
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 291290Lys-63-specific deubiquitinase BRCC36PRO_0000373946Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiB2RYM5.

Expressioni

Gene expression databases

GenevestigatoriB2RYM5.

Interactioni

Subunit structurei

Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas and BRE/BRCC45. Component of the BRISC complex, at least composed of the FAM175B/ABRO1, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. The BRISC complex interacts with the CSN complex. Component of the BRCA1/BRCA2 containing complex (BRCC), which also contains BRCA1, BRCA2, BARD1, BRE and RAD51. BRCC is a ubiquitin E3 ligase complex that enhances cellular survival following DNA damage. Interacts with BRCA1. Binds polyubiquitin (By similarity).By similarity

Protein-protein interaction databases

BioGridi261425. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliB2RYM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 148142MPNAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi122 – 13514JAMM motifAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M67A family. BRCC36 subfamily.Curated
Contains 1 MPN (JAB/Mov34) domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000360.
InParanoidiB2RYM5.
KOiK11864.
OMAiDAFLVCM.
OrthoDBiEOG7KM5TH.
PhylomeDBiB2RYM5.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2RYM5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVPVVQAVQ AVHLESDAFL VCLNHALSTE KEEVMGLCIG ELNDDVRSES
60 70 80 90 100
KFAHAGSDVC TVPEKVDSIR VVHIHSVIIL RRSDKRKDRV EISPEQLSAA
110 120 130 140 150
STEAERLAEL TGRPMRVVGW YHSHPHITVW PSHVDVRTQA MYQMMDQGFV
160 170 180 190 200
GLIFSCFIED KNTKTGRVLY TCFQSVQAQK SSDYERIEIP VHVVPHVTIG
210 220 230 240 250
KVCLESAVEL PKILCQEEQD AYRRIHSLTH LDSVTKIHNG SVFTKNLCSQ
260 270 280 290
MSAVSGPLLQ WLEDRLEQNQ QHLRELQREK EELMAELRSL E
Length:291
Mass (Da):33,016
Last modified:July 1, 2008 - v1
Checksum:i93EB2A7643C70F36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474092 Genomic DNA. Translation: EDL83574.1.
BC166833 mRNA. Translation: AAI66833.1.
RefSeqiNP_001120772.1. NM_001127300.1.
UniGeneiRn.52727.

Genome annotation databases

EnsembliENSRNOT00000071068; ENSRNOP00000066438; ENSRNOG00000048061.
GeneIDi316794.
KEGGirno:316794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474092 Genomic DNA. Translation: EDL83574.1 .
BC166833 mRNA. Translation: AAI66833.1 .
RefSeqi NP_001120772.1. NM_001127300.1.
UniGenei Rn.52727.

3D structure databases

ProteinModelPortali B2RYM5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 261425. 1 interaction.

Protein family/group databases

MEROPSi M67.004.

Proteomic databases

PRIDEi B2RYM5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000071068 ; ENSRNOP00000066438 ; ENSRNOG00000048061 .
GeneIDi 316794.
KEGGi rno:316794.

Organism-specific databases

CTDi 79184.
RGDi 1588543. Brcc3.

Phylogenomic databases

GeneTreei ENSGT00390000000360.
InParanoidi B2RYM5.
KOi K11864.
OMAi DAFLVCM.
OrthoDBi EOG7KM5TH.
PhylomeDBi B2RYM5.

Miscellaneous databases

NextBioi 671241.

Gene expression databases

Genevestigatori B2RYM5.

Family and domain databases

InterProi IPR000555. JAMM/MPN+_dom.
[Graphical view ]
Pfami PF01398. JAB. 1 hit.
[Graphical view ]
SMARTi SM00232. JAB_MPN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiBRCC3_RAT
AccessioniPrimary (citable) accession number: B2RYM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3