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B2RYM5

- BRCC3_RAT

UniProt

B2RYM5 - BRCC3_RAT

Protein

Lys-63-specific deubiquitinase BRCC36

Gene

Brcc3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi122 – 1221Zinc; catalyticBy similarity
    Metal bindingi124 – 1241Zinc; catalyticBy similarity
    Metal bindingi135 – 1351Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. enzyme regulator activity Source: Ensembl
    2. metal ion binding Source: UniProtKB-KW
    3. metallopeptidase activity Source: UniProtKB
    4. polyubiquitin binding Source: UniProtKB
    5. ubiquitin-specific protease activity Source: UniProtKB
    6. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. double-strand break repair Source: UniProtKB
    2. G2 DNA damage checkpoint Source: UniProtKB
    3. histone H2A K63-linked deubiquitination Source: UniProtKB
    4. positive regulation of DNA repair Source: UniProtKB
    5. protein K63-linked deubiquitination Source: UniProtKB
    6. response to ionizing radiation Source: UniProtKB
    7. response to X-ray Source: Ensembl

    Keywords - Molecular functioni

    Chromatin regulator, Hydrolase, Metalloprotease, Protease

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM67.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lys-63-specific deubiquitinase BRCC36 (EC:3.4.19.-)
    Alternative name(s):
    BRCA1-A complex subunit BRCC36
    BRCA1/BRCA2-containing complex subunit 3
    BRCA1/BRCA2-containing complex subunit 36
    BRISC complex subunit BRCC36
    Gene namesi
    Name:Brcc3
    Synonyms:Brcc36
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi1588543. Brcc3.

    Subcellular locationi

    Nucleus By similarity
    Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).By similarity

    GO - Cellular componenti

    1. BRCA1-A complex Source: UniProtKB
    2. BRISC complex Source: UniProtKB
    3. nuclear ubiquitin ligase complex Source: Ensembl
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 291290Lys-63-specific deubiquitinase BRCC36PRO_0000373946Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiB2RYM5.

    Expressioni

    Gene expression databases

    GenevestigatoriB2RYM5.

    Interactioni

    Subunit structurei

    Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas and BRE/BRCC45. Component of the BRISC complex, at least composed of the FAM175B/ABRO1, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. The BRISC complex interacts with the CSN complex. Component of the BRCA1/BRCA2 containing complex (BRCC), which also contains BRCA1, BRCA2, BARD1, BRE and RAD51. BRCC is a ubiquitin E3 ligase complex that enhances cellular survival following DNA damage. Interacts with BRCA1. Binds polyubiquitin By similarity.By similarity

    Protein-protein interaction databases

    BioGridi261425. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliB2RYM5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 148142MPNAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi122 – 13514JAMM motifAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M67A family. BRCC36 subfamily.Curated
    Contains 1 MPN (JAB/Mov34) domain.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000000360.
    KOiK11864.
    OMAiDAFLVCM.
    OrthoDBiEOG7KM5TH.
    PhylomeDBiB2RYM5.

    Family and domain databases

    InterProiIPR000555. JAMM/MPN+_dom.
    [Graphical view]
    PfamiPF01398. JAB. 1 hit.
    [Graphical view]
    SMARTiSM00232. JAB_MPN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B2RYM5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVPVVQAVQ AVHLESDAFL VCLNHALSTE KEEVMGLCIG ELNDDVRSES    50
    KFAHAGSDVC TVPEKVDSIR VVHIHSVIIL RRSDKRKDRV EISPEQLSAA 100
    STEAERLAEL TGRPMRVVGW YHSHPHITVW PSHVDVRTQA MYQMMDQGFV 150
    GLIFSCFIED KNTKTGRVLY TCFQSVQAQK SSDYERIEIP VHVVPHVTIG 200
    KVCLESAVEL PKILCQEEQD AYRRIHSLTH LDSVTKIHNG SVFTKNLCSQ 250
    MSAVSGPLLQ WLEDRLEQNQ QHLRELQREK EELMAELRSL E 291
    Length:291
    Mass (Da):33,016
    Last modified:July 1, 2008 - v1
    Checksum:i93EB2A7643C70F36
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH474092 Genomic DNA. Translation: EDL83574.1.
    BC166833 mRNA. Translation: AAI66833.1.
    RefSeqiNP_001120772.1. NM_001127300.1.
    UniGeneiRn.52727.

    Genome annotation databases

    EnsembliENSRNOT00000071068; ENSRNOP00000066438; ENSRNOG00000048061.
    GeneIDi316794.
    KEGGirno:316794.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH474092 Genomic DNA. Translation: EDL83574.1 .
    BC166833 mRNA. Translation: AAI66833.1 .
    RefSeqi NP_001120772.1. NM_001127300.1.
    UniGenei Rn.52727.

    3D structure databases

    ProteinModelPortali B2RYM5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 261425. 1 interaction.

    Protein family/group databases

    MEROPSi M67.004.

    Proteomic databases

    PRIDEi B2RYM5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000071068 ; ENSRNOP00000066438 ; ENSRNOG00000048061 .
    GeneIDi 316794.
    KEGGi rno:316794.

    Organism-specific databases

    CTDi 79184.
    RGDi 1588543. Brcc3.

    Phylogenomic databases

    GeneTreei ENSGT00390000000360.
    KOi K11864.
    OMAi DAFLVCM.
    OrthoDBi EOG7KM5TH.
    PhylomeDBi B2RYM5.

    Miscellaneous databases

    NextBioi 671241.

    Gene expression databases

    Genevestigatori B2RYM5.

    Family and domain databases

    InterProi IPR000555. JAMM/MPN+_dom.
    [Graphical view ]
    Pfami PF01398. JAB. 1 hit.
    [Graphical view ]
    SMARTi SM00232. JAB_MPN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiBRCC3_RAT
    AccessioniPrimary (citable) accession number: B2RYM5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3