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Protein

Dedicator of cytokinesis protein 5

Gene

Dock5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) for Rho and Rac. GEF proteins activate small GTPases by exchanging bound GDP for free GTP.1 Publication

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 5By similarity
Alternative name(s):
Lens rupture protein 2
Rupture of lens cataract protein
Gene namesi
Name:Dock5Imported
Synonyms:Lr21 Publication, Rlc1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2652871. Dock5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Defects in Dock5 are the cause of rupture of lens cataract. It affects both eyes and is inherited as an autosomal recessive trait. Homozygotes spontaneously develop opacity of the lens at 35-60 days of age. The initial pathological changes appear at about 35 days of age in the deep layer of the posterior cortex as irregular swelling, condensation, degeneration and fragmentation of the lens fibers, leading to rupture of the lens capsule at the posterior pole at 45-100 days of age. Following rupture, the lens nucleus becomes dislocated behind the lens or occasionally in the anterior chamber.

Keywords - Diseasei

Cataract

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18681868Dedicator of cytokinesis protein 5PRO_0000358858Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei365 – 3651PhosphoserineBy similarity
Modified residuei758 – 7581N6-acetyllysineBy similarity
Modified residuei818 – 8181N6-acetyllysineBy similarity
Modified residuei1765 – 17651PhosphoserineBy similarity
Modified residuei1771 – 17711PhosphoserineBy similarity
Modified residuei1788 – 17881PhosphoserineBy similarity
Modified residuei1793 – 17931PhosphothreonineBy similarity
Modified residuei1832 – 18321PhosphoserineBy similarity
Modified residuei1867 – 18671PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiB2RY04.
MaxQBiB2RY04.
PaxDbiB2RY04.
PRIDEiB2RY04.

PTM databases

iPTMnetiB2RY04.
PhosphoSiteiB2RY04.

Expressioni

Tissue specificityi

Highly expressed in lens, where it predominantly localizes to anterior epithelial cells, and is weakly expressed in lens fiber (at protein level). Expressed in brain, eye, lung, spleen and kidney, but not in thymus or peripheral blood leukocytes.1 Publication

Developmental stagei

In the lens, expressed from E15.5 to maturity.1 Publication

Gene expression databases

BgeeiB2RY04.
GenevisibleiB2RY04. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036674.

Structurei

3D structure databases

ProteinModelPortaliB2RY04.
SMRiB2RY04. Positions 1-72, 443-627, 1216-1639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 6962SH3PROSITE-ProRule annotationAdd
BLAST
Domaini443 – 627185DHR-1PROSITE-ProRule annotationAdd
BLAST
Domaini1231 – 1642412DHR-2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1775 – 185379Pro-richSequence analysisAdd
BLAST

Domaini

The DHR-2 domain may mediate some GEF activity.By similarity

Sequence similaritiesi

Belongs to the DOCK family.PROSITE-ProRule annotation
Contains 1 DHR-1 domain.PROSITE-ProRule annotation
Contains 1 DHR-2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG1998. Eukaryota.
ENOG410XQH7. LUCA.
GeneTreeiENSGT00610000085806.
HOGENOMiHOG000006631.
HOVERGENiHBG051389.
InParanoidiB2RY04.
KOiK17707.
OMAiHRSSQET.
OrthoDBiEOG7QNVK8.
TreeFamiTF300423.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR032376. DOCK_N.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 2 hits.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF16172. DOCK_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF50044. SSF50044. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2RY04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARWIPTKRQ KYGVAIYNYN ASQDVELSLQ IGDTVHILEM YEGWYRGYAL
60 70 80 90 100
QNRSKKGIFP ETYIHLKEAT VEDGGQHETV IPGELPLVQE LTNTLREWAV
110 120 130 140 150
IWRKLYVNNK VTLFRQLQQM TYSLIEWRSQ ILSGTLPKDE LAELKKKVTA
160 170 180 190 200
KIDHGNRMLG LDLVVRDDNG NILDPDETST VALFRAHEVA SKRIEEKIQE
210 220 230 240 250
EKSILQNLDL RGQAIFSTVH TYGLYVNFKN FVCNIGEDAE LFIALYDPDQ
260 270 280 290 300
STFISENYLI RWGSNGMPKE IEKLNNLQAV FTDLSSTDLI RPRISLVCQI
310 320 330 340 350
VRVGRMELKE GKKHTCGLRR PFGVAVMDIS DIVHGKVDDE EKQHFIPFQQ
360 370 380 390 400
IAMETYIRQR QLIMSPLITS HVIGENEPLT SVLNKVIAAK EVNHKGQGLW
410 420 430 440 450
VSLKLLPGDL TQVQKNFSHL VDRSTAIARK MGFPEIILPG DVRNDIYVTL
460 470 480 490 500
IHGEFDKGKK KTPKNVEVTM SVFDEEGNLL EKAIHPGAGY EGVSEYKSVV
510 520 530 540 550
YYQVKQPCWY ETVKVFIAIE EVTRCHIRFT FRHRSSQESR DKSERAFGVA
560 570 580 590 600
FVKLMNADGT TLQDGRHDLV VYKGDNKKME DAKYYLTLPG TKAELEEKEL
610 620 630 640 650
QASKNPSVFT PSKDSTKDSF QIATLICSTK LTQNVDLLGL LNWRSNSQNI
660 670 680 690 700
KHNLKKLMEV DGGEIVKFLQ DTLDALFNIM MEMSDNETYD FLVFDALVFI
710 720 730 740 750
ISLIGDIKFQ HFNPVLETYI YKHFSATLAH VKLSKVLNFY VANAEDPSKT
760 770 780 790 800
ELLFAALKAL KYLFRFIIQS RVLYLRFYGQ SEDGDEFNDS IRQLFLAFNT
810 820 830 840 850
LMDRPLEEAV KIKGAALKYL PSIINDVKLV FDPMELSVLF CKFIQSIPDN
860 870 880 890 900
QLVRQKLNCM TKIVESSLFQ QAECREVLLP LLTDQLSGQL DDHSTKPDHE
910 920 930 940 950
ASSQLLSNIL EVLDRTDVGP TSAHVQLIME RLLRRINRTV IGMSRQSPHI
960 970 980 990 1000
GSFVACMIAV LRQMEDSHYS HYISTFKTRQ DIIDFLMETF IMFKDLIGKN
1010 1020 1030 1040 1050
VYAKDWMVMN MTQNRVFLRA INQFAEVLTK SFMDQASFEL QLWNNYFHLA
1060 1070 1080 1090 1100
VAFLTHESLQ LETFSEAKRN KIVKKYGDMR KEIGFRIRDM WYNLGPHKIK
1110 1120 1130 1140 1150
FIPSMVGPIL EVTLTPEVEL RKATIPIFFD MMQCEFNLSG NGNFHMFENE
1160 1170 1180 1190 1200
LITKLDQEVE GGRGDEQYKV LLEKLLLEHC RKHKYLANSG EAFAFLVSSL
1210 1220 1230 1240 1250
LENLLDYRTI IIHDESKENR MSCTVNVLNF YKDKKREDIY IRYLYKLRDL
1260 1270 1280 1290 1300
HRDCENYTEA AYTLLLHAEL LQWSDKPCVP HLLQRDSYYV YTQQELKEKL
1310 1320 1330 1340 1350
YQEIISYFDK GKMWEKAIKL SKELAETYES KVFDYEGLGS LLKKRALFYE
1360 1370 1380 1390 1400
NIIKAMRPQP EYFAVGYYGQ GFPSFLRNKI FIYRGKEYER REDFSLRLLT
1410 1420 1430 1440 1450
QFPNAEKMTS TTPPGEDIKS SPKQYLQCFT VKPVMSLPPS YKDKPVPEQI
1460 1470 1480 1490 1500
LNYYRANEVQ QFSYSRPFRK GEKDPENEFA TMWIERTTYR TAYTFPGILK
1510 1520 1530 1540 1550
WFEAKEISVE EISPLENAIE TMELTNERVS NCVQQHAWDH SLSVHPLSML
1560 1570 1580 1590 1600
LSGIVDPAVM GGFSNYEKAF FTEKYLQEHP EDQEKVELLK RLIALQIPLL
1610 1620 1630 1640 1650
TEGIRIHGEK LTEQLKPLHA RLSSCFRELK EKVEKLYGVI TLPPSMTERK
1660 1670 1680 1690 1700
PSRAGSMVLP YILSSTLRRL SVTSVASSVI STSSNSSDNA SSRPGSDGSI
1710 1720 1730 1740 1750
LEPLFERRAS SGARVEDLPP KEDSENRISK FKRKDWNLSK SQVIAEKAPE
1760 1770 1780 1790 1800
PDVMSPGKKT QRPKSLQLVD SRLTPFHSPS PLQSTALSPP PLTPKATRTL
1810 1820 1830 1840 1850
SSPSLQTDGL TASVPPPPPP KSKPYESSQR NSAEIAPPLP VRRDSKAPPP
1860
PPPKARKSGI LSSEPGSQ
Length:1,868
Mass (Da):214,429
Last modified:July 27, 2011 - v2
Checksum:iD4AB0D8CD588F73D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491A → R in AAI58047 (PubMed:15489334).Curated
Sequence conflicti49 – 491A → R in AAI58136 (PubMed:15489334).Curated
Sequence conflicti49 – 491A → R in AAI58139 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC090431 Genomic DNA. No translation available.
AC093020 Genomic DNA. No translation available.
BC158046 mRNA. Translation: AAI58047.1.
BC158135 mRNA. Translation: AAI58136.1.
BC158138 mRNA. Translation: AAI58139.1.
CCDSiCCDS36963.1.
RefSeqiNP_808448.2. NM_177780.3.
UniGeneiMm.258155.

Genome annotation databases

EnsembliENSMUST00000039135; ENSMUSP00000036674; ENSMUSG00000044447.
GeneIDi68813.
KEGGimmu:68813.
UCSCiuc007ull.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC090431 Genomic DNA. No translation available.
AC093020 Genomic DNA. No translation available.
BC158046 mRNA. Translation: AAI58047.1.
BC158135 mRNA. Translation: AAI58136.1.
BC158138 mRNA. Translation: AAI58139.1.
CCDSiCCDS36963.1.
RefSeqiNP_808448.2. NM_177780.3.
UniGeneiMm.258155.

3D structure databases

ProteinModelPortaliB2RY04.
SMRiB2RY04. Positions 1-72, 443-627, 1216-1639.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036674.

PTM databases

iPTMnetiB2RY04.
PhosphoSiteiB2RY04.

Proteomic databases

EPDiB2RY04.
MaxQBiB2RY04.
PaxDbiB2RY04.
PRIDEiB2RY04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039135; ENSMUSP00000036674; ENSMUSG00000044447.
GeneIDi68813.
KEGGimmu:68813.
UCSCiuc007ull.1. mouse.

Organism-specific databases

CTDi80005.
MGIiMGI:2652871. Dock5.

Phylogenomic databases

eggNOGiKOG1998. Eukaryota.
ENOG410XQH7. LUCA.
GeneTreeiENSGT00610000085806.
HOGENOMiHOG000006631.
HOVERGENiHBG051389.
InParanoidiB2RY04.
KOiK17707.
OMAiHRSSQET.
OrthoDBiEOG7QNVK8.
TreeFamiTF300423.

Miscellaneous databases

ChiTaRSiDock5. mouse.
PROiB2RY04.
SOURCEiSearch...

Gene expression databases

BgeeiB2RY04.
GenevisibleiB2RY04. MM.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR032376. DOCK_N.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 2 hits.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF16172. DOCK_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF50044. SSF50044. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Rupture of lens cataract: a novel hereditary recessive cataract model in the mouse."
    Iida F., Matsushima Y., Hiai H., Uga S., Honda Y.
    Exp. Eye Res. 64:107-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISEASE.
  4. Cited for: DISEASE.
  5. "Mutation of Dock5, a member of the guanine exchange factor Dock180 superfamily, in the rupture of lens cataract mouse."
    Omi N., Kiyokawa E., Matsuda M., Kinoshita K., Yamada S., Yamada K., Matsushima Y., Wang Y., Kawai J., Suzuki M., Hayashizaki Y., Hiai H.
    Exp. Eye Res. 86:828-834(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1867, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiDOCK5_MOUSE
AccessioniPrimary (citable) accession number: B2RY04
Secondary accession number(s): E9QLI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.