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Protein

Plexin-B2

Gene

Plxnb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. Regulates the migration of cerebellar granule cells in the developing brain. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May modulate the activity of RAC1 and CDC42. Down-regulates macrophage migration in wound-healing assays (in vitro).4 Publications

GO - Molecular functioni

  • semaphorin receptor activity Source: UniProtKB

GO - Biological processi

  • brain development Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: MGI
  • negative regulation of cell adhesion Source: GO_Central
  • neural tube closure Source: UniProtKB
  • neuroblast proliferation Source: UniProtKB
  • positive regulation of axonogenesis Source: MGI
  • positive regulation of neuron projection development Source: MGI
  • regulation of cell shape Source: UniProtKB
  • regulation of GTPase activity Source: UniProtKB
  • regulation of neuron migration Source: UniProtKB
  • regulation of protein phosphorylation Source: UniProtKB
  • semaphorin-plexin signaling pathway Source: UniProtKB
  • semaphorin-plexin signaling pathway involved in axon guidance Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Plexin-B2
Gene namesi
Name:Plxnb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2154239. Plxnb2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 1201ExtracellularSequence analysisAdd BLAST1182
Transmembranei1202 – 1222HelicalSequence analysisAdd BLAST21
Topological domaini1223 – 1842CytoplasmicSequence analysisAdd BLAST620

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic and perinatal lethality, due to defects in brain and neural tube development. Mice exhibit abnormal cortical layering and defective migration and differentiation of several subtypes of cortical neurons. Cranial neural folds fail to converge in most embryos, leading to an open neural tube and exencephaly. Likewise, mice exhibit defects in the embryonic development of the cerebellum and the olfactory bulb.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000041571020 – 1842Plexin-B2Add BLAST1823

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi78 ↔ 87PROSITE-ProRule annotation
Disulfide bondi112 ↔ 120PROSITE-ProRule annotation
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi242N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi250 ↔ 366PROSITE-ProRule annotation
Disulfide bondi266 ↔ 313PROSITE-ProRule annotation
Disulfide bondi331 ↔ 353PROSITE-ProRule annotation
Glycosylationi393N-linked (GlcNAc...)1 Publication1
Glycosylationi451N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi471 ↔ 488PROSITE-ProRule annotation
Disulfide bondi477 ↔ 520PROSITE-ProRule annotation
Disulfide bondi480 ↔ 497PROSITE-ProRule annotation
Disulfide bondi491 ↔ 503PROSITE-ProRule annotation
Disulfide bondi557 ↔ 576PROSITE-ProRule annotation
Glycosylationi798N-linked (GlcNAc...)Sequence analysis1
Glycosylationi919N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1053N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1072N-linked (GlcNAc...)Sequence analysis1
Modified residuei1240PhosphoserineCombined sources1
Modified residuei1248PhosphoserineCombined sources1
Modified residuei1574PhosphoserineCombined sources1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1168 – 1169Cleavage; by proprotein convertasesBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiB2RXS4.
PaxDbiB2RXS4.
PeptideAtlasiB2RXS4.
PRIDEiB2RXS4.

PTM databases

iPTMnetiB2RXS4.
PhosphoSitePlusiB2RXS4.
SwissPalmiB2RXS4.

Expressioni

Tissue specificityi

Detected in macrophages from spleen and bone marrow (at protein level). Detected in granule cells in the developing cerebellum, dentate gyrus and olfactory bulb.2 Publications

Gene expression databases

BgeeiENSMUSG00000036606.
GenevisibleiB2RXS4. MM.

Interactioni

Subunit structurei

Monomer, and heterodimer with PLXNB1. Binds MET, ARHGEF11 and ARHGEF12. May also interact with MST1R (By similarity). Interacts with SEMA4C, SEMA4D and SEMA4G.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Arhgef11Q68FM72EBI-8496724,EBI-2365869

Protein-protein interaction databases

BioGridi228283. 2 interactors.
IntActiB2RXS4. 1 interactor.
MINTiMINT-1853052.
STRINGi10090.ENSMUSP00000051731.

Structurei

Secondary structure

11842
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1281 – 1290Combined sources10
Beta strandi1296 – 1300Combined sources5
Turni1301 – 1304Combined sources4
Turni1310 – 1312Combined sources3
Helixi1313 – 1327Combined sources15
Helixi1330 – 1342Combined sources13
Helixi1348 – 1361Combined sources14
Helixi1366 – 1385Combined sources20
Helixi1400 – 1412Combined sources13
Helixi1414 – 1417Combined sources4
Turni1418 – 1421Combined sources4
Helixi1422 – 1437Combined sources16
Turni1443 – 1445Combined sources3
Beta strandi1448 – 1450Combined sources3
Beta strandi1466 – 1474Combined sources9
Beta strandi1479 – 1486Combined sources8
Helixi1491 – 1502Combined sources12
Turni1503 – 1505Combined sources3
Helixi1508 – 1510Combined sources3
Beta strandi1518 – 1521Combined sources4
Beta strandi1532 – 1534Combined sources3
Turni1549 – 1553Combined sources5
Beta strandi1559 – 1563Combined sources5
Beta strandi1592 – 1595Combined sources4
Helixi1623 – 1647Combined sources25
Helixi1656 – 1671Combined sources16
Helixi1677 – 1687Combined sources11
Turni1688 – 1692Combined sources5
Helixi1693 – 1698Combined sources6
Helixi1700 – 1702Combined sources3
Helixi1710 – 1725Combined sources16
Helixi1740 – 1744Combined sources5
Helixi1747 – 1764Combined sources18
Helixi1771 – 1785Combined sources15
Helixi1786 – 1788Combined sources3
Helixi1791 – 1804Combined sources14
Helixi1806 – 1815Combined sources10
Helixi1817 – 1821Combined sources5
Helixi1824 – 1834Combined sources11
Beta strandi1837 – 1841Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5E6PX-ray3.21A1226-1842[»]
ProteinModelPortaliB2RXS4.
SMRiB2RXS4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 468SemaPROSITE-ProRule annotationAdd BLAST449
Domaini806 – 895IPT/TIG 1Add BLAST90
Domaini898 – 982IPT/TIG 2Add BLAST85
Domaini986 – 1095IPT/TIG 3Add BLAST110

Sequence similaritiesi

Belongs to the plexin family.Curated
Contains 3 IPT/TIG domains.Curated
Contains 1 Sema domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KCS6. Eukaryota.
ENOG411131Q. LUCA.
GeneTreeiENSGT00760000119048.
HOGENOMiHOG000231376.
HOVERGENiHBG053404.
InParanoidiB2RXS4.
KOiK06821.
OMAiEPKQGPQ.
OrthoDBiEOG091G00EK.
PhylomeDBiB2RXS4.
TreeFamiTF312962.

Family and domain databases

Gene3Di1.10.506.10. 2 hits.
2.130.10.10. 1 hit.
2.60.40.10. 3 hits.
InterProiIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR031148. Plexin.
IPR013548. Plexin_cytoplasmic_RasGAP_dom.
IPR002165. Plexin_repeat.
IPR016201. PSI.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001627. Semap_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR22625. PTHR22625. 2 hits.
PfamiPF08337. Plexin_cytopl. 1 hit.
PF01437. PSI. 2 hits.
PF01403. Sema. 1 hit.
PF01833. TIG. 3 hits.
[Graphical view]
SMARTiSM00429. IPT. 3 hits.
SM00423. PSI. 3 hits.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF48350. SSF48350. 2 hits.
SSF81296. SSF81296. 3 hits.
PROSITEiPS51004. SEMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2RXS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPLWALTF LGLTGLGLSL RSRKPESFRS ETELNHLAVD EVTGVVYVGA
60 70 80 90 100
VNALYQLSAD LHVQQHVVTG PFMDNKKCTP PIEASQCHEA VLTDNFNQLL
110 120 130 140 150
LLDPPGKRLV ECGSLFKGIC ALRAMSNISV RLFYEDGSGE KSFVASNDER
160 170 180 190 200
VATVGLVTST RPDGERVLFV GKGNGPHDNG IIVSTRLLDR AEGREAFEAY
210 220 230 240 250
SDHTTFKAGY LSTNTQQFVA AFEDDFYVFF VFNHQDKHPA KNRTLLARMC
260 270 280 290 300
KDDPSYYSYV EMDLQCQDPS DPQDSAFGTC LAASVATSGA GRALYAVFSR
310 320 330 340 350
DGRSTGGPGA GLCVFPLDKV REKIEANRNA CYTGAREAGR TIFYKPFHGE
360 370 380 390 400
IQCGGHLIGA SESFPCGSEH LPYPLGSRDG LVATAVLHRG GLNLTAVTVT
410 420 430 440 450
AENDHTVAFL GTSDGRILKV YLAPDGTSAE YGSIPVDINK KIKQDLALSG
460 470 480 490 500
NLSSLYAMTQ DKVFRLPVQE CLSYVTCAQC RDSQDPYCGW CVIEGRCTRK
510 520 530 540 550
SECSRAEETG HWLWSREKSC VAITDAFPQN MSRRAQGEVR LSVSPLPTLT
560 570 580 590 600
EDDELLCLFG DSPPHPARVE DDTVICNSPS SIPSTPPGQD HVDVSIQLLL
610 620 630 640 650
KSGSVFLTSH QYPFYDCREA MSLVENLPCI SCASNRWTCQ WDLQYYECRE
660 670 680 690 700
ASPNPEEGII RAHMEDNCPQ FLAPDPLVIP MNHETEVTFQ GKNLETVKVS
710 720 730 740 750
SLYVGSELLN FEETVTMHES DTFSFRTPKL SHDGNETLPL HLYVKSFGKN
760 770 780 790 800
IDSKLQVTLY NCSFGRSDCS LCLAADPAYR CVWCRGQNRC VYEALCSNVT
810 820 830 840 850
SECPPPVITR IQPETGPLGG GILVTIHGSN LGVKADDVKK ITVAGQNCAF
860 870 880 890 900
EPRGYSVSTR IVCAIEASEM PFTGGIEVDV NGKLGHSPPH VQFTYQQPQP
910 920 930 940 950
LSVEPRQGPQ AGGTTLTING THLDTGSKED VRVTLNDVPC EVTKFGAQLQ
960 970 980 990 1000
CVTGQQLAPG QVTLEIYYGG SRVPSPGISF TYCENPMIRA FEPLRSFVSG
1010 1020 1030 1040 1050
GRSINVTGQG FSLIQKFAMV VIAEPLRSWR RRRREAGALE RVTVEGMEYV
1060 1070 1080 1090 1100
FYNDTKVVFL SPAVPEEPEA YNLTVLIRMD GHCAPLRTEA GVFEYVADPT
1110 1120 1130 1140 1150
FENFTGGVKK QVNKLIHARG TNLNKAMTLE EAEAFVGAER CIMKTLTETD
1160 1170 1180 1190 1200
LYCEPPEVQP PPKRRQKRDT AHNLPEFIVK FGSREWVLGR VEYDTRASDV
1210 1220 1230 1240 1250
PLSLILPLVM VPMVFIIVVS IYCYWRKSQQ AEREYEKIKS QLEGLEESVR
1260 1270 1280 1290 1300
DRCKKEFTDL MIEMEDQTND VHEAGIPTLD YKTYTDRVFF LPSKDGDKDV
1310 1320 1330 1340 1350
MITGKLDIPE SRRPIVEQAL YQFSNLLNSK SFLINFIHTL ENQREFSARA
1360 1370 1380 1390 1400
KVYFASLLTV ALHGKLEYYT DIMRTLFLEL MEQYVVAKNP KLMLRRSETV
1410 1420 1430 1440 1450
VERMLSNWMS ICLYQYLKDS AGEPLYKLFK AIKHQVEKGP VDAVQKKAKY
1460 1470 1480 1490 1500
TLNDTGLLGD DVEYAPLTVS VIVQDEGIDA IPVKVLNCDT ISQVKEKIID
1510 1520 1530 1540 1550
QVYRTQPCSC WPKPDSVVLE WRPGSTAQIL SDLDLTSQRE GRWKRINTLM
1560 1570 1580 1590 1600
HYNVRDGATL ILSKVGVSQQ PEDSQQDLPG ERHALLEEEN RVWHLVRPTD
1610 1620 1630 1640 1650
EVDEGKSKRG SMKEKERTKA ITEIYLTRLL SVKGTLQQFV DNFFQSVLAP
1660 1670 1680 1690 1700
GHAVPPAVKY FFDFLDEQAE KHDIRDEDTI HIWKTNSLPL RFWVNILKNP
1710 1720 1730 1740 1750
HFIFDVHVHE VVDASLSVIA QTFMDACTRT EHKLSRDSPS NKLLYAKEIS
1760 1770 1780 1790 1800
TYKKMVEDYY KGIRQMVQVS DQDMNTHLAE ISRAHTDSLN TLVALHQLYQ
1810 1820 1830 1840
YTQKYYDEII NALEEDPAAQ KMQLAFRLQQ IAAALENKVT DL
Length:1,842
Mass (Da):206,230
Last modified:July 1, 2008 - v1
Checksum:i0D445E7354C107FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1382E → G in BAE27981 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147538 mRNA. Translation: BAE27981.1.
AC113069 Genomic DNA. No translation available.
AC160538 Genomic DNA. No translation available.
CH466550 Genomic DNA. Translation: EDL04367.1.
BC157960 mRNA. Translation: AAI57961.1.
BC157961 mRNA. Translation: AAI57962.1.
BC158086 mRNA. Translation: AAI58087.1.
BC172162 mRNA. Translation: AAI72162.1.
CCDSiCCDS49698.1.
RefSeqiNP_001152993.1. NM_001159521.2.
NP_001271435.1. NM_001284506.1.
NP_620088.2. NM_138749.3.
XP_006520475.1. XM_006520412.1.
XP_006520476.1. XM_006520413.1.
XP_011243751.1. XM_011245449.1.
XP_011243752.1. XM_011245450.2.
XP_011243753.1. XM_011245451.2.
UniGeneiMm.267514.

Genome annotation databases

EnsembliENSMUST00000060808; ENSMUSP00000051731; ENSMUSG00000036606.
ENSMUST00000109331; ENSMUSP00000104955; ENSMUSG00000036606.
GeneIDi140570.
KEGGimmu:140570.
UCSCiuc007xfr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147538 mRNA. Translation: BAE27981.1.
AC113069 Genomic DNA. No translation available.
AC160538 Genomic DNA. No translation available.
CH466550 Genomic DNA. Translation: EDL04367.1.
BC157960 mRNA. Translation: AAI57961.1.
BC157961 mRNA. Translation: AAI57962.1.
BC158086 mRNA. Translation: AAI58087.1.
BC172162 mRNA. Translation: AAI72162.1.
CCDSiCCDS49698.1.
RefSeqiNP_001152993.1. NM_001159521.2.
NP_001271435.1. NM_001284506.1.
NP_620088.2. NM_138749.3.
XP_006520475.1. XM_006520412.1.
XP_006520476.1. XM_006520413.1.
XP_011243751.1. XM_011245449.1.
XP_011243752.1. XM_011245450.2.
XP_011243753.1. XM_011245451.2.
UniGeneiMm.267514.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5E6PX-ray3.21A1226-1842[»]
ProteinModelPortaliB2RXS4.
SMRiB2RXS4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228283. 2 interactors.
IntActiB2RXS4. 1 interactor.
MINTiMINT-1853052.
STRINGi10090.ENSMUSP00000051731.

PTM databases

iPTMnetiB2RXS4.
PhosphoSitePlusiB2RXS4.
SwissPalmiB2RXS4.

Proteomic databases

MaxQBiB2RXS4.
PaxDbiB2RXS4.
PeptideAtlasiB2RXS4.
PRIDEiB2RXS4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060808; ENSMUSP00000051731; ENSMUSG00000036606.
ENSMUST00000109331; ENSMUSP00000104955; ENSMUSG00000036606.
GeneIDi140570.
KEGGimmu:140570.
UCSCiuc007xfr.2. mouse.

Organism-specific databases

CTDi23654.
MGIiMGI:2154239. Plxnb2.

Phylogenomic databases

eggNOGiENOG410KCS6. Eukaryota.
ENOG411131Q. LUCA.
GeneTreeiENSGT00760000119048.
HOGENOMiHOG000231376.
HOVERGENiHBG053404.
InParanoidiB2RXS4.
KOiK06821.
OMAiEPKQGPQ.
OrthoDBiEOG091G00EK.
PhylomeDBiB2RXS4.
TreeFamiTF312962.

Miscellaneous databases

ChiTaRSiPlxnb2. mouse.
PROiB2RXS4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036606.
GenevisibleiB2RXS4. MM.

Family and domain databases

Gene3Di1.10.506.10. 2 hits.
2.130.10.10. 1 hit.
2.60.40.10. 3 hits.
InterProiIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR031148. Plexin.
IPR013548. Plexin_cytoplasmic_RasGAP_dom.
IPR002165. Plexin_repeat.
IPR016201. PSI.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001627. Semap_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR22625. PTHR22625. 2 hits.
PfamiPF08337. Plexin_cytopl. 1 hit.
PF01437. PSI. 2 hits.
PF01403. Sema. 1 hit.
PF01833. TIG. 3 hits.
[Graphical view]
SMARTiSM00429. IPT. 3 hits.
SM00423. PSI. 3 hits.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF48350. SSF48350. 2 hits.
SSF81296. SSF81296. 3 hits.
PROSITEiPS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLXB2_MOUSE
AccessioniPrimary (citable) accession number: B2RXS4
Secondary accession number(s): Q3UH76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 1, 2008
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.